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Title: Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins

Abstract

Water quality is an important determinant for the structural integrity of alloy cladded fuels and assemblies during long-term wet storage. Detailed characterization of a water filled storage basin for spent nuclear reactor fuel was performed following the formation and proliferation of an amorphous white flocculent. White precipitant was sampled throughout the storage basin for chemical and spectroscopic characterization, and environmental DNA was extracted for 454 pyrosequencing of bacterial 16S rRNA gene diversity. Accordingly, spectroscopic analyses indicated the precipitant to be primarily amorphous to crystalline aluminum (oxy) hydroxides with minor associated elemental components including Fe, Si, Ti, and U. High levels of organic carbon were co-localized with the precipitant relative to bulk dissolved organic concentrations. Bacterial densities were highly variable between sampling locations and with depth within the water filled storage basin; cell numbers ranged from 4 × 10 3to 4 × 104 cells/mL. Bacterial diversity that was physically associated with the aluminum (oxy) hydroxide complexes exceeded an estimated 4,000 OTUs/amplicon library (3% cutoff) and the majority of sequences were aligned to the families Burkholderiaceae (23%), Nitrospiraceae (23%), Hyphomicrobiaceae (17%), and Comamonadaceae (6%). We surmise that episodic changes in the physical and chemical properties of the basin contribute to the polymerizationmore » of aluminum (oxy) hydroxides, which in turn can chemisorb nutrients, carbon ligands and bacterial cells from the surrounding bulk aqueous phase. As such, these precipitants should establish favorable microhabitats for bacterial colonization and growth. Comparative analyses of 16S rRNA gene amplicon libraries across a selection of natural and engineered aquatic ecosystems were performed and microbial community and taxonomic signatures unique to the spent nuclear fuel (SNF) storage basin environment were revealed. These insights could spur the development of tractable bio-indicators that are specific of and diagnostic for water quality at discrete locations and finer scales of resolution, marking an important contribution for improved water quality and management of SNF storage facilities.« less

Authors:
 [1];  [2];  [3];  [3];  [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Washington, Seattle, WA (United States)
  3. Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
Publication Date:
Research Org.:
Savannah River Site (SRS), Aiken, SC (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1439101
Alternate Identifier(s):
OSTI ID: 1427456
Report Number(s):
SRNL-STI-2017-00717; PNNL-SA-130553
Journal ID: ISSN 1664-302X; TRN: US1802461
Grant/Contract Number:  
AC09-08SR22470; AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES; bacterial diversity; spent nuclear fuel; aluminum; amplicon; signatures

Citation Formats

Bagwell, Christopher E., Noble, Peter A., Milliken, Charles E., Li, Dien, and Kaplan, Daniel I. Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.00377.
Bagwell, Christopher E., Noble, Peter A., Milliken, Charles E., Li, Dien, & Kaplan, Daniel I. Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins. United States. doi:10.3389/fmicb.2018.00377.
Bagwell, Christopher E., Noble, Peter A., Milliken, Charles E., Li, Dien, and Kaplan, Daniel I. Fri . "Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins". United States. doi:10.3389/fmicb.2018.00377. https://www.osti.gov/servlets/purl/1439101.
@article{osti_1439101,
title = {Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins},
author = {Bagwell, Christopher E. and Noble, Peter A. and Milliken, Charles E. and Li, Dien and Kaplan, Daniel I.},
abstractNote = {Water quality is an important determinant for the structural integrity of alloy cladded fuels and assemblies during long-term wet storage. Detailed characterization of a water filled storage basin for spent nuclear reactor fuel was performed following the formation and proliferation of an amorphous white flocculent. White precipitant was sampled throughout the storage basin for chemical and spectroscopic characterization, and environmental DNA was extracted for 454 pyrosequencing of bacterial 16S rRNA gene diversity. Accordingly, spectroscopic analyses indicated the precipitant to be primarily amorphous to crystalline aluminum (oxy) hydroxides with minor associated elemental components including Fe, Si, Ti, and U. High levels of organic carbon were co-localized with the precipitant relative to bulk dissolved organic concentrations. Bacterial densities were highly variable between sampling locations and with depth within the water filled storage basin; cell numbers ranged from 4 × 103to 4 × 104 cells/mL. Bacterial diversity that was physically associated with the aluminum (oxy) hydroxide complexes exceeded an estimated 4,000 OTUs/amplicon library (3% cutoff) and the majority of sequences were aligned to the families Burkholderiaceae (23%), Nitrospiraceae (23%), Hyphomicrobiaceae (17%), and Comamonadaceae (6%). We surmise that episodic changes in the physical and chemical properties of the basin contribute to the polymerization of aluminum (oxy) hydroxides, which in turn can chemisorb nutrients, carbon ligands and bacterial cells from the surrounding bulk aqueous phase. As such, these precipitants should establish favorable microhabitats for bacterial colonization and growth. Comparative analyses of 16S rRNA gene amplicon libraries across a selection of natural and engineered aquatic ecosystems were performed and microbial community and taxonomic signatures unique to the spent nuclear fuel (SNF) storage basin environment were revealed. These insights could spur the development of tractable bio-indicators that are specific of and diagnostic for water quality at discrete locations and finer scales of resolution, marking an important contribution for improved water quality and management of SNF storage facilities.},
doi = {10.3389/fmicb.2018.00377},
journal = {Frontiers in Microbiology},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {3}
}

Journal Article:
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Figures / Tables:

FIGURE 1 FIGURE 1: Underwater view of spent nuclear fuel canisters supported in a storage rack. Anomalous flocculent can be seen spanning the tops of individual fuel canisters and spreading between them along the storage rack.

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