Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle

Kramer, Philip A.; Duan, Jicheng; Gaffrey, Matthew J.; Shukla, Anil K.; Wang, Lu; Bammler, Theo K.; Qian, Wei -Jun; Marcinek, David J.

doi:10.1016/j.redox.2018.05.011

Title: Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle

Abstract

Protein S-glutathionylation is an important reversible post-translational modification implicated in redox signaling. Oxidative modifications to protein thiols can alter the activity of metabolic enzymes, transcription factors, kinases, phosphatases, and the function of contractile proteins. However, the extent to which muscle contraction induces oxidative modifications in redox sensitive thiols is not known. The purpose of this study was to determine the targets of S-glutathionylation redox signaling following fatiguing contractions. Anesthetized adult male CB6F1 (BALB/cBy × C57BL/6) mice were subjected to acute fatiguing contractions for 15 min using in vivo stimulations. The right (stimulated) and left (unstimulated) gastrocnemius muscleswere collected 60 min after the last stimulation and processed for redox proteomics assay of S-glutathionylation.

Authors:

Kramer, Philip A. ^[1]; Duan, Jicheng ^[2]; Gaffrey, Matthew J. ^[2]; Shukla, Anil K. ^[2]; Wang, Lu ^[1]; Bammler, Theo K. ^[1]; Qian, Wei -Jun ^[2]; Marcinek, David J. ^[1]

Univ. of Washington, Seattle, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Publication Date:: 2018-05-23

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1439099

Report Number(s):: PNNL-SA-135523
Journal ID: ISSN 2213-2317; PII: S2213231718303550

Grant/Contract Number:: AC05-76RL01830

Resource Type:: Accepted Manuscript

Journal Name:: Redox Biology

Additional Journal Information:: Journal Volume: 17; Journal Issue: C; Journal ID: ISSN 2213-2317

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Muscle contraction; Post-translational modification; S-glutathionylation; Redox signaling; Skeletal muscle; Thiol redox proteomics

Citation Formats


                    Kramer, Philip A., Duan, Jicheng, Gaffrey, Matthew J., Shukla, Anil K., Wang, Lu, Bammler, Theo K., Qian, Wei -Jun, and Marcinek, David J. Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle.  United States: N. p., 2018. 
        Web.  doi:10.1016/j.redox.2018.05.011.

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                    Kramer, Philip A., Duan, Jicheng, Gaffrey, Matthew J., Shukla, Anil K., Wang, Lu, Bammler, Theo K., Qian, Wei -Jun, & Marcinek, David J. Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle.  United States.  doi:https://doi.org/10.1016/j.redox.2018.05.011

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                    Kramer, Philip A., Duan, Jicheng, Gaffrey, Matthew J., Shukla, Anil K., Wang, Lu, Bammler, Theo K., Qian, Wei -Jun, and Marcinek, David J. Wed .  
        "Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle".  United States.  doi:https://doi.org/10.1016/j.redox.2018.05.011.  https://www.osti.gov/servlets/purl/1439099.

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@article{osti_1439099,

  title        = {Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle},

  author       = {Kramer, Philip A. and Duan, Jicheng and Gaffrey, Matthew J. and Shukla, Anil K. and Wang, Lu and Bammler, Theo K. and Qian, Wei -Jun and Marcinek, David J.},

  abstractNote = {Protein S-glutathionylation is an important reversible post-translational modification implicated in redox signaling. Oxidative modifications to protein thiols can alter the activity of metabolic enzymes, transcription factors, kinases, phosphatases, and the function of contractile proteins. However, the extent to which muscle contraction induces oxidative modifications in redox sensitive thiols is not known. The purpose of this study was to determine the targets of S-glutathionylation redox signaling following fatiguing contractions. Anesthetized adult male CB6F1 (BALB/cBy × C57BL/6) mice were subjected to acute fatiguing contractions for 15 min using in vivo stimulations. The right (stimulated) and left (unstimulated) gastrocnemius muscleswere collected 60 min after the last stimulation and processed for redox proteomics assay of S-glutathionylation.},

  doi          = {10.1016/j.redox.2018.05.011},

  journal      = {Redox Biology},

  number       = C,

  volume       = 17,

  place        = {United States},

  year         = {2018},

  month        = {5}

}

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Journal Article:

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DOI: https://doi.org/10.1016/j.redox.2018.05.011

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Cited by: 9 works

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Figures / Tables:

Table 1: Known glutathionylation sites associated with altered protein function. Table of P-SSG modifications significantly affected by fatiguing contractions sorted by Excitation/contraction Coupling, Metabolism, Redox Regulation, and Other. Significance determined by p-value (≤ 0.05). Note: Some P-SSG sites were no longer significant with FDR correction.

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Works referencing / citing this record:

Isotopically Labeled Clickable Glutathione to Quantify Protein S‐Glutathionylation
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Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2
journal, July 2019

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DOI: 10.3390/antiox8080245

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

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Title: Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle

Abstract

Citation Formats

Figures / Tables:

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journal, October 2019

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journal, July 2019