Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

Hou, Caixia; Biswas, Tapan; Tsodikov, Oleg V.

doi:10.1021/acs.biochem.8b00036

Title: Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

Journal Article · 20 March 2018 · Biochemistry

DOI: https://doi.org/10.1021/acs.biochem.8b00036 · OSTI ID:1438848

Hou, Caixia ^[1]; Biswas, Tapan ^[2];

^[1]

Univ. of Kentucky, Lexington, KY (United States)
Univ. of California, San Diego, La Jolla, CA (United States)

Bacterial primase DnaG is an essential nucleic acid polymerase that generates primers for replication of chromosomal DNA. The mechanism of DnaG remains unclear due to the paucity of structural information on DnaG in complexes with other replisome components. Furthermore we report the first crystal structures of noncovalent DnaG–DNA complexes, obtained with the RNA polymerase domain of Mycobacterium tuberculosis DnaG and various DNA ligands. One structure, obtained with ds DNA, reveals interactions with DnaG as it slides on ds DNA and suggests how DnaG binds template for primer synthesis. In another structure, DNA in the active site of DnaG mimics the primer, providing insight into mechanisms for the nucleotide transfer and DNA translocation. In conjunction with the recent cryo-EM structure of the bacteriophage T7 replisome, this study yields a model for primer elongation and hand-off to DNA polymerase.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: SER-CAT

OSTI ID:: 1438848

Journal Information:: Biochemistry, Journal Name: Biochemistry Journal Issue: 14 Vol. 57; ISSN 0006-2960

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
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crystal structure
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peptides and proteins
replisome
tuberculosis

Title: Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

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