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Title: MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula

Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula. MtSWEET11 belongs to a clade of plant SWEET proteins that are capable of transporting Suc and play critical roles in pathogen susceptibility. When expressed in mammalian cells, MtSWEET11 transported sucrose (Suc) but not glucose (Glc). The MtSWEET11 gene was found to be expressed in infected root hair cells, and in the meristem, invasion zone, and vasculature of nodules. Expression of an MtSWEET11-GFP fusion protein in nodules resulted in green fluorescence associated with the plasma membrane of uninfected cells and infection thread and symbiosome membranes of infected cells. Two independent Tnt1-insertion sweet11 mutants were uncompromised in SNF. Furthermore, although MtSWEET11 appears to be involved in Suc distribution within nodules, it is not crucial for SNF, probably because other Suc transporters can fulfill its role(s).
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [1] ;  [2] ; ORCiD logo [1] ;  [3] ; ORCiD logo [3] ; ORCiD logo [4] ;  [2] ; ORCiD logo [1]
  1. The Samuel Roberts Foundation, Ardmore, OK (United States)
  2. Carnegie Inst. of Science, Stanford, CA (United States)
  3. John Innes Centre, Norwich (United Kingdom)
  4. West Virginia Univ., Morgantown, WV (United States)
Publication Date:
Grant/Contract Number:
FG02-04ER15542
Type:
Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda); Journal Volume: 171; Journal Issue: 1; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Research Org:
Carnegie Inst. of Science, Stanford, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1438839

Kryvoruchko, Igor S., Sinharoy, Senjuti, Torres-Jerez, Ivone, Sosso, Davide, Pislariu, Catalina I., Guan, Dian, Murray, Jeremy, Benedito, Vagner A., Frommer, Wolf B., and Udvardi, Michael K.. MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula. United States: N. p., Web. doi:10.1104/pp.15.01910.
Kryvoruchko, Igor S., Sinharoy, Senjuti, Torres-Jerez, Ivone, Sosso, Davide, Pislariu, Catalina I., Guan, Dian, Murray, Jeremy, Benedito, Vagner A., Frommer, Wolf B., & Udvardi, Michael K.. MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula. United States. doi:10.1104/pp.15.01910.
Kryvoruchko, Igor S., Sinharoy, Senjuti, Torres-Jerez, Ivone, Sosso, Davide, Pislariu, Catalina I., Guan, Dian, Murray, Jeremy, Benedito, Vagner A., Frommer, Wolf B., and Udvardi, Michael K.. 2016. "MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula". United States. doi:10.1104/pp.15.01910. https://www.osti.gov/servlets/purl/1438839.
@article{osti_1438839,
title = {MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula},
author = {Kryvoruchko, Igor S. and Sinharoy, Senjuti and Torres-Jerez, Ivone and Sosso, Davide and Pislariu, Catalina I. and Guan, Dian and Murray, Jeremy and Benedito, Vagner A. and Frommer, Wolf B. and Udvardi, Michael K.},
abstractNote = {Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula. MtSWEET11 belongs to a clade of plant SWEET proteins that are capable of transporting Suc and play critical roles in pathogen susceptibility. When expressed in mammalian cells, MtSWEET11 transported sucrose (Suc) but not glucose (Glc). The MtSWEET11 gene was found to be expressed in infected root hair cells, and in the meristem, invasion zone, and vasculature of nodules. Expression of an MtSWEET11-GFP fusion protein in nodules resulted in green fluorescence associated with the plasma membrane of uninfected cells and infection thread and symbiosome membranes of infected cells. Two independent Tnt1-insertion sweet11 mutants were uncompromised in SNF. Furthermore, although MtSWEET11 appears to be involved in Suc distribution within nodules, it is not crucial for SNF, probably because other Suc transporters can fulfill its role(s).},
doi = {10.1104/pp.15.01910},
journal = {Plant Physiology (Bethesda)},
number = 1,
volume = 171,
place = {United States},
year = {2016},
month = {3}
}