High-throughput platforms for metabolomics

de Raad, Markus; Fischer, Curt R.; Northen, Trent R.

doi:10.1016/j.cbpa.2015.10.012

Title: High-throughput platforms for metabolomics

Journal Article · Wed Nov 04 00:00:00 UTC 2015 · Current Opinion in Chemical Biology

DOI: https://doi.org/10.1016/j.cbpa.2015.10.012 · OSTI ID:1437950

de Raad, Markus ^[1]; Fischer, Curt R. ^[1]; Northen, Trent R. ^[1]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

We report that mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we highlight current high-throughput MS-based platforms and their potential application in metabolomics. Although current MS platforms can reach throughputs up to 0.5. seconds per sample, the metabolite coverage of these platforms are low compared to low-throughput, separation-based MS methods. High-throughput comes at a cost, as it's a trade-off between sample throughput and metabolite coverage. As we will discuss, promising emerging technologies, including microfluidics and miniaturization of separation techniques, have the potential to achieve both rapid and more comprehensive metabolite analysis.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1437950

Journal Information:: Current Opinion in Chemical Biology, Journal Name: Current Opinion in Chemical Biology Journal Issue: C Vol. 30; ISSN 1367-5931

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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