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Title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Journal Article · · Acta Crystallographica. Section F, Structural Biology Communications
 [1];  [2]; ORCiD logo [3]; ORCiD logo [3];  [1]
  1. Nebraska Medical Center, Omaha, NE (United States). Eppley Inst. for Research in Cancer and Allied Diseases, Dept. of Biochemistry and Molecular Biology
  2. Nebraska Medical Center, Omaha, NE (United States). Eppley Inst. for Research in Cancer and Allied Diseases
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology and Soft Matter Division

Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collectedvianeutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Aeronautics and Space Administration (NASA); National Institutes of Health (NIH)
Grant/Contract Number:
AC05-00OR22725; 44-0307-1021-201; P30CA036727; 5P20RR016469
OSTI ID:
1437709
Alternate ID(s):
OSTI ID: 1407982
Journal Information:
Acta Crystallographica. Section F, Structural Biology Communications, Vol. 73, Issue 4; ISSN 2053-230X
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

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Cited By (8)

Neutron macromolecular crystallography journal February 2018
A suite-level review of the neutron single-crystal diffraction instruments at Oak Ridge National Laboratory journal September 2018
Instrument and shielding design of a neutron diffractometer at J-PARC for protein crystallography covering crystals with large unit-cell volume journal April 2018
Redox manipulation of the manganese metal in human manganese superoxide dismutase for neutron diffraction journal September 2018
Neutron crystallographic study of heterotrimeric glutamine amidotransferase CAB journal February 2019
Neutron scattering in the biological sciences: progress and prospects journal December 2018
A Review of the Catalytic Mechanism of Human Manganese Superoxide Dismutase journal January 2018
Neutron Macromolecular Crystallography book January 2020

Figures / Tables (5)