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Title: Glycoside hydrolase gene transcription by Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and monosaccharides: a proposed xylan hydrolysis mechanism

Metabolism of carbon bound in wheat arabinoxylan (WAX) polysaccharides by bacteria requires a number of glycoside hydrolases active toward different bonds between sugars and other molecules. Alicyclobacillus acidocaldarius is a Gram-positive thermoacidophilic bacterium capable of growth on a variety of mono-, di-, oligo-, and polysaccharides. Nineteen proposed glycoside hydrolases have been annotated in the A. acidocaldarius Type Strain ATCC27009/DSM 446 genome. Here, experiments were performed to understand the effect of monosaccharides on gene expression during growth on the polysaccharide, WAX.
Authors:
ORCiD logo [1] ;  [2] ;  [3] ;  [4]
  1. Idaho National Lab. (INL), Idaho Falls, ID (United States); Idaho State Univ., Pocatello, ID (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Idaho National Lab. (INL), Idaho Falls, ID (United States)
  3. Idaho State Univ., Pocatello, ID (United States)
  4. New Mexico Institute of Mining and Technology, Socorro, NM (United States)
Publication Date:
Report Number(s):
PNNL-SA-121820
Journal ID: ISSN 1754-6834; PII: 1110
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Alicyclobacillus acidocaldarius; Wheat arabinoxylan; Glycoside hydrolase; Microarray
OSTI Identifier:
1437038