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Title: Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs inhibit not only the enzyme activities but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes. While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL-tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. Here, we review recent progress on discovering IL-tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4]
  1. Univ. of California, Davis, CA (United States). Dept. of Biological and Agricultural Engineering
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Science and Engineering Division
  3. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biology and Biotechnology Division
  4. Univ. of California, Davis, CA (United States). Dept. of Biological and Agricultural Engineering; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 100; Journal Issue: 24; Journal ID: ISSN 0175-7598
Publisher:
Springer
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); US Dept. of Agriculture (USDA) National Inst. of Food and Agriculture (NIFA)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Ionic liquid tolerance; Microbial community enrichment; Ionic liquid pretreatment; Engineering ionic liquid tolerance
OSTI Identifier:
1436606

Yu, Chaowei, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., and VanderGheynst, Jean S.. Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts. United States: N. p., Web. doi:10.1007/s00253-016-7955-0.
Yu, Chaowei, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., & VanderGheynst, Jean S.. Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts. United States. doi:10.1007/s00253-016-7955-0.
Yu, Chaowei, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., and VanderGheynst, Jean S.. 2016. "Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts". United States. doi:10.1007/s00253-016-7955-0. https://www.osti.gov/servlets/purl/1436606.
@article{osti_1436606,
title = {Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts},
author = {Yu, Chaowei and Simmons, Blake A. and Singer, Steven W. and Thelen, Michael P. and VanderGheynst, Jean S.},
abstractNote = {Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs inhibit not only the enzyme activities but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes. While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL-tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. Here, we review recent progress on discovering IL-tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.},
doi = {10.1007/s00253-016-7955-0},
journal = {Applied Microbiology and Biotechnology},
number = 24,
volume = 100,
place = {United States},
year = {2016},
month = {11}
}

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