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Title: Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
Authors:
ORCiD logo [1] ;  [2] ; ORCiD logo [3]
  1. Zhejiang Univ., Hangzhou (China). Key Lab. of Biomass Chemical Engineering of Ministry of Education and College of Chemical and Biological Engineering; Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering and Carl R. Woese Inst. for Genomic Biology
  2. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering and Carl R. Woese Inst. for Genomic Biology
  3. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering, Carl R. Woese Inst. for Genomic Biology, and Dept. of Chemistry, Biochemistry and Bioengineering
Publication Date:
Grant/Contract Number:
SC0018420; SC0018260
Type:
Accepted Manuscript
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Name: Biotechnology Journal; Journal ID: ISSN 1860-6768
Research Org:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Zhejiang Univ., Hangzhou (China)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; gene regulation; genome engineering; metabolic engineering; saccaromyces cerevisiae; synthetic biology; Saccharomyces cerevisiae
OSTI Identifier:
1436426
Alternate Identifier(s):
OSTI ID: 1433560; OSTI ID: 1435935

Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States: N. p., Web. doi:10.1002/biot.201700601.
Lian, Jiazhang, HamediRad, Mohammad, & Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States. doi:10.1002/biot.201700601.
Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. 2018. "Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System". United States. doi:10.1002/biot.201700601.
@article{osti_1436426,
title = {Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System},
author = {Lian, Jiazhang and HamediRad, Mohammad and Zhao, Huimin},
abstractNote = {Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.},
doi = {10.1002/biot.201700601},
journal = {Biotechnology Journal},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Works referenced in this record:

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
journal, February 2013

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
journal, July 2013

DNA targeting specificity of RNA-guided Cas9 nucleases
journal, July 2013
  • Hsu, Patrick D.; Scott, David A.; Weinstein, Joshua A.
  • Nature Biotechnology, Vol. 31, Issue 9, p. 827-832
  • DOI: 10.1038/nbt.2647

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
journal, October 2015
  • Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O.
  • Cell, Vol. 163, Issue 3, p. 759-771
  • DOI: 10.1016/j.cell.2015.09.038

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
journal, March 2013
  • DiCarlo, James E.; Norville, Julie E.; Mali, Prashant
  • Nucleic Acids Research, Vol. 41, Issue 7, p. 4336-4343
  • DOI: 10.1093/nar/gkt135

RNA-Guided Human Genome Engineering via Cas9
journal, January 2013

Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
journal, December 2016
  • Garst, Andrew D.; Bassalo, Marcelo C.; Pines, Gur
  • Nature Biotechnology, Vol. 35, Issue 1, p. 48-55
  • DOI: 10.1038/nbt.3718