Capturing the genetic makeup of the active microbiome in situ
Abstract
More than any other technology, nucleic acid sequencing has enabled microbial ecology studies to be complemented with the data volumes necessary to capture the extent of microbial diversity and dynamics in a wide range of environments. In order to truly understand and predict environmental processes, however, the distinction between active, inactive and dead microbial cells is critical. Also, experimental designs need to be sensitive toward varying population complexity and activity, and temporal as well as spatial scales of process rates. There are a number of approaches, including single-cell techniques, which were designed to study in situ microbial activity and that have been successively coupled to nucleic acid sequencing. The exciting new discoveries regarding in situ microbial activity provide evidence that future microbial ecology studies will indispensably rely on techniques that specifically capture members of the microbiome active in the environment. Herein, we review those currently used activity-based approaches that can be directly linked to shotgun nucleic acid sequencing, evaluate their relevance to ecology studies, and discuss future directions.
- Authors:
-
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Univ. of Vienna (Austria). Dept. of Microbial Ecology and Ecosystem Science. Division of Microbial Ecology
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); European Research Council (ERC)
- OSTI Identifier:
- 1436339
- Grant/Contract Number:
- AC02-05CH11231; 294343
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 11; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; environmental microbiology; microbial ecology
Citation Formats
Singer, Esther, Wagner, Michael, and Woyke, Tanja. Capturing the genetic makeup of the active microbiome in situ. United States: N. p., 2017.
Web. doi:10.1038/ismej.2017.59.
Singer, Esther, Wagner, Michael, & Woyke, Tanja. Capturing the genetic makeup of the active microbiome in situ. United States. https://doi.org/10.1038/ismej.2017.59
Singer, Esther, Wagner, Michael, and Woyke, Tanja. Fri .
"Capturing the genetic makeup of the active microbiome in situ". United States. https://doi.org/10.1038/ismej.2017.59. https://www.osti.gov/servlets/purl/1436339.
@article{osti_1436339,
title = {Capturing the genetic makeup of the active microbiome in situ},
author = {Singer, Esther and Wagner, Michael and Woyke, Tanja},
abstractNote = {More than any other technology, nucleic acid sequencing has enabled microbial ecology studies to be complemented with the data volumes necessary to capture the extent of microbial diversity and dynamics in a wide range of environments. In order to truly understand and predict environmental processes, however, the distinction between active, inactive and dead microbial cells is critical. Also, experimental designs need to be sensitive toward varying population complexity and activity, and temporal as well as spatial scales of process rates. There are a number of approaches, including single-cell techniques, which were designed to study in situ microbial activity and that have been successively coupled to nucleic acid sequencing. The exciting new discoveries regarding in situ microbial activity provide evidence that future microbial ecology studies will indispensably rely on techniques that specifically capture members of the microbiome active in the environment. Herein, we review those currently used activity-based approaches that can be directly linked to shotgun nucleic acid sequencing, evaluate their relevance to ecology studies, and discuss future directions.},
doi = {10.1038/ismej.2017.59},
journal = {The ISME Journal},
number = ,
volume = 11,
place = {United States},
year = {Fri Jun 02 00:00:00 EDT 2017},
month = {Fri Jun 02 00:00:00 EDT 2017}
}
Web of Science
Works referenced in this record:
Analysis of methanotrophic bacteria in Movile Cave by stable isotope probing: Methanotrophs in Movile Cave
journal, December 2003
- Hutchens, Elena; Radajewski, Stefan; Dumont, Marc G.
- Environmental Microbiology, Vol. 6, Issue 2
The energetics of anabolism in natural settings
journal, February 2016
- LaRowe, Douglas E.; Amend, Jan P.
- The ISME Journal, Vol. 10, Issue 6
The phenomenon of microbial uncultivability
journal, October 2013
- Epstein, Ss
- Current Opinion in Microbiology, Vol. 16, Issue 5
Anvi’o: an advanced analysis and visualization platform for ‘omics data
journal, January 2015
- Eren, A. Murat; Esen, Özcan C.; Quince, Christopher
- PeerJ, Vol. 3
Bacterial carbon processing by generalist species in the coastal ocean
journal, January 2008
- Mou, Xiaozhen; Sun, Shulei; Edwards, Robert A.
- Nature, Vol. 451, Issue 7179
Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate
journal, February 2016
- De Filippis, Francesca; Genovese, Alessandro; Ferranti, Pasquale
- Scientific Reports, Vol. 6, Issue 1
Marine methylotrophs revealed by stable-isotope probing, multiple displacement amplification and metagenomics: Metagenomics of active marine methyotrophs
journal, February 2008
- Neufeld, Josh D.; Chen, Yin; Dumont, Marc G.
- Environmental Microbiology, Vol. 10, Issue 6
Functional diversity in resource use by fungi
journal, August 2010
- McGuire, Krista L.; Bent, Elizabeth; Borneman, James
- Ecology, Vol. 91, Issue 8
Metatranscriptome analyses indicate resource partitioning between diatoms in the field
journal, April 2015
- Alexander, Harriet; Jenkins, Bethany D.; Rynearson, Tatiana A.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 17
Raman activated cell sorting
journal, August 2016
- Song, Yizhi; Yin, Huabing; Huang, Wei E.
- Current Opinion in Chemical Biology, Vol. 33
Stable-isotope probing as a tool in microbial ecology
journal, February 2000
- Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R.
- Nature, Vol. 403, Issue 6770, p. 646-649
Single cell stable isotope probing in microbiology using Raman microspectroscopy
journal, October 2016
- Wang, Yun; Huang, Wei E.; Cui, Li
- Current Opinion in Biotechnology, Vol. 41
Fungal Taxa Target Different Carbon Sources in Forest Soil
journal, September 2008
- Hanson, China A.; Allison, Steven D.; Bradford, Mark A.
- Ecosystems, Vol. 11, Issue 7
Diversity and activity of marine bacterioplankton during a diatom bloom in the North Sea assessed by total RNA and pyrotag sequencing
journal, December 2014
- Klindworth, Anna; Mann, Alexander J.; Huang, Sixing
- Marine Genomics, Vol. 18
Active bacterial community structure along vertical redox gradients in Baltic Sea sediment
journal, August 2008
- Edlund, Anna; Hrdeman, Fredrik; Jansson, Janet K.
- Environmental Microbiology, Vol. 10, Issue 8
Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community
journal, October 2013
- Embree, Mallory; Nagarajan, Harish; Movahedi, Narjes
- The ISME Journal, Vol. 8, Issue 4
Respiration Response Imaging for Real-Time Detection of Microbial Function at the Single-Cell Level
journal, November 2010
- Konopka, M. C.; Strovas, T. J.; Ojala, David S.
- Applied and Environmental Microbiology, Vol. 77, Issue 1
Heavy water and 15 N labelling with NanoSIMS analysis reveals growth rate-dependent metabolic heterogeneity in chemostats : NanoSIMS analysis of metabolic diversity in chemostats
journal, March 2015
- Kopf, Sebastian H.; McGlynn, Shawn E.; Green-Saxena, Abigail
- Environmental Microbiology, Vol. 17, Issue 7
Combining metagenomics, metatranscriptomics and viromics to explore novel microbial interactions: towards a systems-level understanding of human microbiome
journal, January 2015
- Bikel, Shirley; Valdez-Lara, Alejandra; Cornejo-Granados, Fernanda
- Computational and Structural Biotechnology Journal, Vol. 13
Characterization of a Novel Methanol Dehydrogenase in Representatives of Burkholderiales: Implications for Environmental Detection of Methylotrophy and Evidence for Convergent Evolution
journal, April 2008
- Kalyuzhnaya, M. G.; Hristova, K. R.; Lidstrom, M. E.
- Journal of Bacteriology, Vol. 190, Issue 11
Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses
journal, July 2013
- Blazewicz, Steven J.; Barnard, Romain L.; Daly, Rebecca A.
- The ISME Journal, Vol. 7, Issue 11
The metatranscriptome of a deep-sea hydrothermal plume is dominated by water column methanotrophs and lithotrophs
journal, June 2012
- Lesniewski, Ryan A.; Jain, Sunit; Anantharaman, Karthik
- The ISME Journal, Vol. 6, Issue 12
Measuring unbiased metatranscriptomics in suboxic waters of the central Baltic Sea using a new in situ fixation system
journal, July 2011
- Feike, Janie; Jürgens, Klaus; Hollibaugh, James T.
- The ISME Journal, Vol. 6, Issue 2
Bacterial Functional Redundancy along a Soil Reclamation Gradient
journal, October 2000
- Yin, B.; Crowley, D.; Sparovek, G.
- Applied and Environmental Microbiology, Vol. 66, Issue 10
Archaea predominate among ammonia-oxidizing prokaryotes in soils
journal, August 2006
- Leininger, S.; Urich, T.; Schloter, M.
- Nature, Vol. 442, Issue 7104
DNA stable-isotope probing
journal, April 2007
- Neufeld, Josh D.; Vohra, Jyotsna; Dumont, Marc G.
- Nature Protocols, Vol. 2, Issue 4
Uncultured archaea in deep marine subsurface sediments: have we caught them all?
journal, November 2007
- Teske, Andreas; Sørensen, Ketil B.
- The ISME Journal, Vol. 2, Issue 1
Fungi in Cystic Fibrosis: Recent Findings and Unresolved Questions
journal, December 2014
- Eickmeier, Olaf; Hector, Andreas; Singh, Anurag
- Current Fungal Infection Reports, Vol. 9, Issue 1
Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release
journal, February 2015
- Zhang, Peiran; Ren, Lihui; Zhang, Xu
- Analytical Chemistry, Vol. 87, Issue 4
Active populations of rare microbes in oceanic environments as revealed by bromodeoxyuridine incorporation and 454 tag sequencing
journal, February 2016
- Hamasaki, Koji; Taniguchi, Akito; Tada, Yuya
- Gene, Vol. 576, Issue 2
Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon
journal, March 2010
- Poretsky, Rachel S.; Sun, Shulei; Mou, Xiaozhen
- Environmental Microbiology, Vol. 12, Issue 3
Advancements in the application of NanoSIMS and Raman microspectroscopy to investigate the activity of microbial cells in soils
journal, August 2015
- Eichorst, Stephanie A.; Strasser, Florian; Woyke, Tanja
- FEMS Microbiology Ecology, Vol. 91, Issue 10
Raman Activated Cell Ejection for Isolation of Single Cells
journal, October 2013
- Wang, Yun; Ji, Yuetong; Wharfe, Emma S.
- Analytical Chemistry, Vol. 85, Issue 22
Metagenomics and metatranscriptomics: Windows on CF-associated viral and microbial communities
journal, March 2013
- Lim, Yan Wei; Schmieder, Robert; Haynes, Matthew
- Journal of Cystic Fibrosis, Vol. 12, Issue 2
Rhizosphere microbiome assemblage is affected by plant development
journal, November 2013
- Chaparro, Jacqueline M.; Badri, Dayakar V.; Vivanco, Jorge M.
- The ISME Journal, Vol. 8, Issue 4
Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques
journal, March 2003
- Xie, Changan; Li, Yong-qing
- Journal of Applied Physics, Vol. 93, Issue 5
Paracatenula, an ancient symbiosis between thiotrophic Alphaproteobacteria and catenulid flatworms
journal, June 2011
- Gruber-Vodicka, H. R.; Dirks, U.; Leisch, N.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 29
Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia
journal, June 2016
- Hatzenpichler, Roland; Connon, Stephanie A.; Goudeau, Danielle
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 28
Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer
posted_content, April 2016
- Simpson, Jared T.; Workman, Rachael; Zuzarte, P. C.
Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms
journal, June 2003
- Radajewski, Stefan; McDonald, Ian R.; Murrell, J. Colin
- Current Opinion in Biotechnology, Vol. 14, Issue 3, p. 296-302
Novel mycoviruses discovered from metatranscriptomics survey of soybean phyllosphere phytobiomes
journal, February 2016
- Marzano, Shin-Yi Lee; Domier, Leslie L.
- Virus Research, Vol. 213
Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples
journal, July 2015
- Korem, T.; Zeevi, D.; Suez, J.
- Science, Vol. 349, Issue 6252
Drivers of bacterial colonization patterns in stream biofilms
journal, April 2010
- Augspurger, Clemens; Karwautz, Clemens; Mußmann, Marc
- FEMS Microbiology Ecology, Vol. 72, Issue 1
A novel fungal family of oligopeptide transporters identified by functional metatranscriptomics of soil eukaryotes
journal, June 2011
- Damon, Coralie; Vallon, Laurent; Zimmermann, Sabine
- The ISME Journal, Vol. 5, Issue 12
DNA sequencing with nanopores
journal, April 2012
- Schneider, Grégory F.; Dekker, Cees
- Nature Biotechnology, Vol. 30, Issue 4
Single-cell genomic sequencing using Multiple Displacement Amplification
journal, October 2007
- Lasken, Roger S.
- Current Opinion in Microbiology, Vol. 10, Issue 5
Scaling up: the next challenge in environmental microbiology
journal, November 2003
- Paerl, Hans W.; Steppe, Timothy F.
- Environmental Microbiology, Vol. 5, Issue 11
Identification of Functionally Relevant Populations in Enhanced Biological Phosphorus Removal Processes Based On Intracellular Polymers Profiles and Insights into the Metabolic Diversity and Heterogeneity
journal, April 2012
- Majed, Nehreen; Chernenko, Tatyana; Diem, Max
- Environmental Science & Technology, Vol. 46, Issue 9
Searching for new branches on the tree of life
journal, November 2014
- Woyke, T.; Rubin, E. M.
- Science, Vol. 346, Issue 6210
RNA stable-isotope probing
journal, April 2007
- Whiteley, Andrew S.; Thomson, Bruce; Lueders, Tillmann
- Nature Protocols, Vol. 2, Issue 4
High-resolution metagenomics targets specific functional types in complex microbial communities
journal, August 2008
- Kalyuzhnaya, Marina G.; Lapidus, Alla; Ivanova, Natalia
- Nature Biotechnology, Vol. 26, Issue 9
The activity level of a microbial community function can be predicted from its metatranscriptome
journal, November 2011
- Helbling, Damian E.; Ackermann, Martin; Fenner, Kathrin
- The ISME Journal, Vol. 6, Issue 4
Coupled RNA-SIP and metatranscriptomics of active chemolithoautotrophic communities at a deep-sea hydrothermal vent
journal, February 2016
- Fortunato, Caroline S.; Huber, Julie A.
- The ISME Journal, Vol. 10, Issue 8
Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP
journal, June 2013
- Gutierrez, Tony; Singleton, David R.; Berry, David
- The ISME Journal, Vol. 7, Issue 11
Observations on the Influence of Deuterium on Bacterial Growth*
journal, November 1960
- Lester, William; Sun, Sung Huang; Seber, Almera
- Annals of the New York Academy of Sciences, Vol. 84, Issue 16
Microbial ecology of chlorinated solvent biodegradation: Microbial ecology of PCE degradation
journal, March 2014
- David, Maude M.; Cecillon, Sebastien; Warne, Brett M.
- Environmental Microbiology, Vol. 17, Issue 12
SIP metagenomics identifies uncultivated Methylophilaceae as dimethylsulphide degrading bacteria in soil and lake sediment
journal, March 2015
- Eyice, Özge; Namura, Motonobu; Chen, Yin
- The ISME Journal, Vol. 9, Issue 11
De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities
journal, June 2011
- Narasingarao, Priya; Podell, Sheila; Ugalde, Juan A.
- The ISME Journal, Vol. 6, Issue 1
Protein-based stable isotope probing
journal, November 2010
- Jehmlich, Nico; Schmidt, Frank; Taubert, Martin
- Nature Protocols, Vol. 5, Issue 12
On the Origins and Control of Community Types in the Human Microbiome
journal, February 2016
- Gibson, Travis E.; Bashan, Amir; Cao, Hong-Tai
- PLOS Computational Biology, Vol. 12, Issue 2
Stable isotope probing with 18O-water to investigate microbial growth and death in environmental samples
journal, October 2016
- Schwartz, Egbert; Hayer, Michaela; Hungate, Bruce A.
- Current Opinion in Biotechnology, Vol. 41
Relating the metatranscriptome and metagenome of the human gut
journal, May 2014
- Franzosa, E. A.; Morgan, X. C.; Segata, N.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 22
The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata
journal, December 2011
- Pagani, I.; Liolios, K.; Jansson, J.
- Nucleic Acids Research, Vol. 40, Issue D1
Zero-valent sulphur is a key intermediate in marine methane oxidation
journal, November 2012
- Milucka, Jana; Ferdelman, Timothy G.; Polerecky, Lubos
- Nature, Vol. 491, Issue 7425
Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics
journal, November 2014
- Hua, Zheng-Shuang; Han, Yu-Jiao; Chen, Lin-Xing
- The ISME Journal, Vol. 9, Issue 6
Gene expression in the deep biosphere
journal, June 2013
- Orsi, William D.; Edgcomb, Virginia P.; Christman, Glenn D.
- Nature, Vol. 499, Issue 7457
Deep subseafloor microbial cells on physiological standby
journal, October 2011
- Jorgensen, B. B.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 45
Microbial life under extreme energy limitation
journal, January 2013
- Hoehler, Tori M.; Jørgensen, Bo Barker
- Nature Reviews Microbiology, Vol. 11, Issue 2
Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT)
journal, June 2006
- Dieterich, D. C.; Link, A. J.; Graumann, J.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 25
Enrichment of specific protozoan populations during in situ bioremediation of uranium-contaminated groundwater
journal, February 2013
- Holmes, Dawn E.; Giloteaux, Ludovic; Williams, Kenneth H.
- The ISME Journal, Vol. 7, Issue 7
A ‘rare biosphere’ microorganism contributes to sulfate reduction in a peatland
journal, June 2010
- Pester, Michael; Bittner, Norbert; Deevong, Pinsurang
- The ISME Journal, Vol. 4, Issue 12
Acceleration and Trapping of Particles by Radiation Pressure
journal, January 1970
- Ashkin, A.
- Physical Review Letters, Vol. 24, Issue 4
Recombination is a key driver of genomic and phenotypic diversity in a Pseudomonas aeruginosa population during cystic fibrosis infection
journal, January 2015
- Darch, Sophie E.; McNally, Alan; Harrison, Freya
- Scientific Reports, Vol. 5, Issue 1
Meta-Analysis of Quantification Methods Shows that Archaea and Bacteria Have Similar Abundances in the Subseafloor
journal, October 2013
- Lloyd, Karen G.; May, Megan K.; Kevorkian, Richard T.
- Applied and Environmental Microbiology, Vol. 79, Issue 24
Real-time detection of actively metabolizing microbes by redox sensing as applied to methylotroph populations in Lake Washington
journal, March 2008
- Kalyuzhnaya, Marina G.; Lidstrom, Mary E.; Chistoserdova, Ludmila
- The ISME Journal, Vol. 2, Issue 7
Raman-FISH: combining stable-isotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function
journal, August 2007
- Huang, Wei E.; Stoecker, Kilian; Griffiths, Robert
- Environmental Microbiology, Vol. 9, Issue 8
Global dispersion and local diversification of the methane seep microbiome
journal, March 2015
- Ruff, S. Emil; Biddle, Jennifer F.; Teske, Andreas P.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 13
Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates
journal, September 2011
- Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.
- Journal of Microbiological Methods, Vol. 86, Issue 3
Metatranscriptomic analysis of diverse microbial communities reveals core metabolic pathways and microbiome-specific functionality
journal, January 2016
- Jiang, Yue; Xiong, Xuejian; Danska, Jayne
- Microbiome, Vol. 4, Issue 1
Single-Molecule Tracing on a Fluidic Microchip for Quantitative Detection of Low-Abundance Nucleic Acids
journal, April 2005
- Wang, Tza-Huei; Peng, Yahui; Zhang, Chunyang
- Journal of the American Chemical Society, Vol. 127, Issue 15
Identification of a complete methane monooxygenase operon from soil by combining stable isotope probing and metagenomic analysis
journal, July 2006
- Dumont, Marc G.; Radajewski, Stefan M.; Miguez, Carlos B.
- Environmental Microbiology, Vol. 8, Issue 7
Simultaneous Assessment of Soil Microbial Community Structure and Function through Analysis of the Meta-Transcriptome
journal, June 2008
- Urich, Tim; Lanzén, Anders; Qi, Ji
- PLoS ONE, Vol. 3, Issue 6
Diel gene expression profiles of a phosphorus limited mountain lake using metatranscriptomics: Diel metatranscriptomic approach of a mountain lake
journal, November 2012
- Vila-Costa, Maria; Sharma, Shalabh; Moran, Mary Ann
- Environmental Microbiology, Vol. 15, Issue 4
Revealing the uncultivated majority: combining DNA stable-isotope probing, multiple displacement amplification and metagenomic analyses of uncultivated Methylocystis in acidic peatlands: Uncultivated Methylocystis in acidic peatlands
journal, July 2008
- Chen, Yin; Dumont, Marc G.; Neufeld, Josh D.
- Environmental Microbiology, Vol. 10, Issue 10
Validation of two ribosomal RNA removal methods for microbial metatranscriptomics
journal, September 2010
- He, Shaomei; Wurtzel, Omri; Singh, Kanwar
- Nature Methods, Vol. 7, Issue 10
In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry : Visualizing new proteins in microbes by click chemistry
journal, April 2014
- Hatzenpichler, Roland; Scheller, Silvan; Tavormina, Patricia L.
- Environmental Microbiology, Vol. 16, Issue 8
Metagenomics: Application of Genomics to Uncultured Microorganisms
journal, December 2004
- Handelsman, J.
- Microbiology and Molecular Biology Reviews, Vol. 68, Issue 4
Microbial community gene expression within colonies of the diazotroph, Trichodesmium, from the Southwest Pacific Ocean
journal, July 2009
- Hewson, Ian; Poretsky, Rachel S.; Dyhrman, Sonya T.
- The ISME Journal, Vol. 3, Issue 11
Microbial Community Dynamics and Stability during an Ammonia-Induced Shift to Syntrophic Acetate Oxidation
journal, March 2014
- Werner, Jeffrey J.; Garcia, Marcelo L.; Perkins, Sarah D.
- Applied and Environmental Microbiology, Vol. 80, Issue 11
Microbial community gene expression in ocean surface waters
journal, March 2008
- Frias-Lopez, J.; Shi, Y.; Tyson, G. W.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 10
Gene expression of lactobacilli in murine forestomach biofilms: Autochthonous murine forestomach biofilms
journal, April 2014
- Schwab, Clarissa; Tveit, Alexander Tøsdal; Schleper, Christa
- Microbial Biotechnology, Vol. 7, Issue 4
A first comprehensive census of fungi in soil reveals both hyperdiversity and fine-scale niche partitioning
journal, February 2014
- Taylor, D. Lee; Hollingsworth, Teresa N.; McFarland, Jack W.
- Ecological Monographs, Vol. 84, Issue 1
Community composition and activity state of estuarine bacterioplankton assessed using differential staining and metagenomic analysis of 16S rDNA and rRNA
journal, July 2013
- Franklin, Rb; Luria, C.; Shozo Ozaki, L.
- Aquatic Microbial Ecology, Vol. 69, Issue 3
A Conversation on Refining the Concept of Keystone Species
journal, August 1995
- Paine, R. T.
- Conservation Biology, Vol. 9, Issue 4
Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology
journal, October 2006
- Neufeld, Josh D.; Dumont, Marc G.; Vohra, Jyotsna
- Microbial Ecology, Vol. 53, Issue 3
The human gut microbiome and its dysfunctions through the meta-omics prism: Meta-omics of the human gut microbiome
journal, March 2016
- Mondot, Stanislas; Lepage, Patricia
- Annals of the New York Academy of Sciences, Vol. 1372, Issue 1
Community transcriptomic assembly reveals microbes that contribute to deep-sea carbon and nitrogen cycling
journal, May 2013
- Baker, Brett J.; Sheik, Cody S.; Taylor, Chris A.
- The ISME Journal, Vol. 7, Issue 10
Microspatial gene expression patterns in the Amazon River Plume
journal, July 2014
- Satinsky, B. M.; Crump, B. C.; Smith, C. B.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 30
Metatranscriptomics reveals overall active bacterial composition in caries lesions
journal, January 2014
- Simón-Soro, Aurea; Guillen-Navarro, Miriam; Mira, Alex
- Journal of Oral Microbiology, Vol. 6, Issue 1
Metatranscriptomics of N2-fixing cyanobacteria in the Amazon River plume
journal, December 2014
- Hilton, Jason A.; Satinsky, Brandon M.; Doherty, Mary
- The ISME Journal, Vol. 9, Issue 7
Metatranscriptomics reveal differences in in situ energy and nitrogen metabolism among hydrothermal vent snail symbionts
journal, April 2013
- Sanders, J. G.; Beinart, R. A.; Stewart, F. J.
- The ISME Journal, Vol. 7, Issue 8
DNA replication initiation: mechanisms and regulation in bacteria
journal, May 2007
- Mott, Melissa L.; Berger, James M.
- Nature Reviews Microbiology, Vol. 5, Issue 5
Microbial activity and soil respiration under nitrogen addition in Alaskan boreal forest
journal, May 2008
- Allison, Steven D.; Czimczik, Claudia I.; Treseder, Kathleen K.
- Global Change Biology, Vol. 14, Issue 5
Raman tweezers sorting of single microbial cells
journal, February 2009
- Huang, Wei E.; Ward, Andrew D.; Whiteley, Andrew S.
- Environmental Microbiology Reports, Vol. 1, Issue 1
Anoxic carbon flux in photosynthetic microbial mats as revealed by metatranscriptomics
journal, November 2012
- Burow, Luke C.; Woebken, Dagmar; Marshall, Ian PG
- The ISME Journal, Vol. 7, Issue 4
Plant growth in Arabidopsis is assisted by compost soil-derived microbial communities
journal, January 2013
- Carvalhais, Lilia C.; Muzzi, Frederico; Tan, Chin-Hong
- Frontiers in Plant Science, Vol. 4
A renaissance for the pioneering 16S rRNA gene
journal, October 2008
- Tringe, Susannah G.; Hugenholtz, Philip
- Current Opinion in Microbiology, Vol. 11, Issue 5
Quantitative analysis of a deeply sequenced marine microbial metatranscriptome
journal, September 2010
- Gifford, Scott M.; Sharma, Shalabh; Rinta-Kanto, Johanna M.
- The ISME Journal, Vol. 5, Issue 3
Towards high-throughput microfluidic Raman-activated cell sorting
journal, January 2015
- Zhang, Qiang; Zhang, Peiran; Gou, Honglei
- The Analyst, Vol. 140, Issue 18
Metagenomics uncovers gaps in amplicon-based detection of microbial diversity
journal, February 2016
- Eloe-Fadrosh, Emiley A.; Ivanova, Natalia N.; Woyke, Tanja
- Nature Microbiology, Vol. 1, Issue 4
Functional group-specific traits drive phytoplankton dynamics in the oligotrophic ocean
journal, October 2015
- Alexander, Harriet; Rouco, Mónica; Haley, Sheean T.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 44
Warming and drying suppress microbial activity and carbon cycling in boreal forest soils
journal, December 2008
- Allison, Steven D.; Treseder, Kathleen K.
- Global Change Biology, Vol. 14, Issue 12
Combined bromodeoxyuridine immunocapture and terminal-restriction fragment length polymorphism analysis highlights differences in the active soil bacterial metagenome due to Glomus mosseae inoculation or plant species: Active soil bacterial metagenome
journal, July 2005
- Artursson, Veronica; Finlay, Roger D.; Jansson, Janet K.
- Environmental Microbiology, Vol. 7, Issue 12
Tracking heavy water (D 2 O) incorporation for identifying and sorting active microbial cells
journal, December 2014
- Berry, David; Mader, Esther; Lee, Tae Kwon
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 2
Microbial seed banks: the ecological and evolutionary implications of dormancy
journal, January 2011
- Lennon, Jay T.; Jones, Stuart E.
- Nature Reviews Microbiology, Vol. 9, Issue 2
The Guild Concept and the Structure of Ecological Communities
journal, November 1991
- Simberloff, D.; Dayan, T.
- Annual Review of Ecology and Systematics, Vol. 22, Issue 1
Single-cell technologies in environmental omics
journal, February 2016
- Kodzius, Rimantas; Gojobori, Takashi
- Gene, Vol. 576, Issue 2
Differential Growth Responses of Soil Bacterial Taxa to Carbon Substrates of Varying Chemical Recalcitrance
journal, January 2011
- Goldfarb, Katherine C.; Karaoz, Ulas; Hanson, China A.
- Frontiers in Microbiology, Vol. 2
RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny
journal, November 2002
- Manefield, M.; Whiteley, A. S.; Griffiths, R. I.
- Applied and Environmental Microbiology, Vol. 68, Issue 11
Spliced leader-based metatranscriptomic analyses lead to recognition of hidden genomic features in dinoflagellates
journal, November 2010
- Lin, S.; Zhang, H.; Zhuang, Y.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 46
Metatranscriptomics of the Human Oral Microbiome during Health and Disease
journal, February 2014
- Jorth, P.; Turner, K. H.; Gumus, P.
- mBio, Vol. 5, Issue 2
DNA-Stable Isotope Probing Integrated with Metagenomics for Retrieval of Biphenyl Dioxygenase Genes from Polychlorinated Biphenyl-Contaminated River Sediment
journal, July 2009
- Sul, W. J.; Park, J.; Quensen, J. F.
- Applied and Environmental Microbiology, Vol. 75, Issue 17
In Situ Replication Rates for Uncultivated Bacteria in Microbial Communities
posted_content, June 2016
- Brown, Christopher T.; Olm, Matthew R.; Thomas, Brian C.
Xenobiotics and the Human Gut Microbiome: Metatranscriptomics Reveal the Active Players
journal, March 2013
- Ursell, Luke K.; Knight, Rob
- Cell Metabolism, Vol. 17, Issue 3
Fungal invasion of the rhizosphere microbiome
journal, May 2015
- Chapelle, Emilie; Mendes, Rodrigo; Bakker, Peter A. HM
- The ISME Journal, Vol. 10, Issue 1
The function of our microbiota: who is out there and what do they do?
journal, January 2012
- Ottman, Noora; Smidt, Hauke; de Vos, Willem M.
- Frontiers in Cellular and Infection Microbiology, Vol. 2
Structure and activity of multiple nitrifying bacterial populations co-existing in a biofilm
journal, May 2003
- Gieseke, Armin; Bjerrum, Lotte; Wagner, Michael
- Environmental Microbiology, Vol. 5, Issue 5
Sizing up metatranscriptomics
journal, August 2012
- Moran, Mary Ann; Satinsky, Brandon; Gifford, Scott M.
- The ISME Journal, Vol. 7, Issue 2
Recovering glycoside hydrolase genes from active tundra cellulolytic bacteria
journal, July 2014
- Pinnell, Lee J.; Dunford, Eric; Ronan, Patrick
- Canadian Journal of Microbiology, Vol. 60, Issue 7
Metatranscriptomics reveals unique microbial small RNAs in the ocean’s water column
journal, May 2009
- Shi, Yanmei; Tyson, Gene W.; DeLong, Edward F.
- Nature, Vol. 459, Issue 7244
Microbial Scout Hypothesis, Stochastic Exit from Dormancy, and the Nature of Slow Growers
journal, February 2012
- Buerger, S.; Spoering, A.; Gavrish, E.
- Applied and Environmental Microbiology, Vol. 78, Issue 9
Metatranscriptomic insights into polyphosphate metabolism in marine sediments
journal, September 2015
- Jones, Daniel S.; Flood, Beverly E.; Bailey, Jake V.
- The ISME Journal, Vol. 10, Issue 4
Culture-Independent Identification of Microorganisms That Respond to Specified Stimuli
journal, August 1999
- Borneman, James
- Applied and Environmental Microbiology, Vol. 65, Issue 8
Immunochemical Detection and Isolation of DNA from Metabolically Active Bacteria
journal, March 1999
- Urbach, Ena; Vergin, Kevin L.; Giovannoni, Stephen J.
- Applied and Environmental Microbiology, Vol. 65, Issue 3
Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP
text, January 2013
- David, Berry,; D., Aitken, Michael; Tony, Gutierrez,
- The University of North Carolina at Chapel Hill University Libraries
Single-cell bacterial transcription measurements reveal the importance of dimethylsulfoniopropionate (DMSP) hotspots in ocean sulfur cycling
text, January 2020
- Gao, Cherry; Fernandez, Vicente I.; Lee, Kang S.
- ETH Zurich
Global dispersion and local diversification of the methane seep microbiome
text, January 2015
- Teske, Andreas P.; Ramette, Alban Nicolas; Boetius, Antje
- National Academy of Sciences NAS
Drivers of bacterial colonization patterns in stream biofilms
journal, January 2010
- Augspurger, Clemens; Karwautz, Clemens; Mußmann, Marc
- FEMS Microbiology Ecology
Gene expression of lactobacilli in murine forestomach biofilms
text, January 2014
- Schwab, Clarissa; Tveit, Alexander T.; Schleper, Christa
- ETH Zurich
Metagenomics: Application of Genomics to Uncultured Microorganisms
journal, March 2005
- Handelsman, Jo
- Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1
Erratum: Community transcriptomic assembly reveals microbes that contribute to deep-sea carbon and nitrogen cycling
journal, September 2013
- Baker, Brett J.; Sheik, Cody S.; Taylor, Chris A.
- The ISME Journal, Vol. 7, Issue 10
Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology
journal, October 2006
- Neufeld, Josh D.; Dumont, Marc G.; Vohra, Jyotsna
- Microbial Ecology, Vol. 53, Issue 3
Raman activated cell sorting
journal, August 2016
- Song, Yizhi; Yin, Huabing; Huang, Wei E.
- Current Opinion in Chemical Biology, Vol. 33
Xenobiotics Shape the Physiology and Gene Expression of the Active Human Gut Microbiome
journal, January 2013
- Maurice, Corinne Ferrier; Haiser, Henry Joseph; Turnbaugh, Peter James
- Cell, Vol. 152, Issue 1-2
Combining metagenomics, metatranscriptomics and viromics to explore novel microbial interactions: towards a systems-level understanding of human microbiome
journal, January 2015
- Bikel, Shirley; Valdez-Lara, Alejandra; Cornejo-Granados, Fernanda
- Computational and Structural Biotechnology Journal, Vol. 13
Active populations of rare microbes in oceanic environments as revealed by bromodeoxyuridine incorporation and 454 tag sequencing
journal, February 2016
- Hamasaki, Koji; Taniguchi, Akito; Tada, Yuya
- Gene, Vol. 576, Issue 2
Single-cell technologies in environmental omics
journal, February 2016
- Kodzius, Rimantas; Gojobori, Takashi
- Gene, Vol. 576, Issue 2
Metagenomics and metatranscriptomics: Windows on CF-associated viral and microbial communities
journal, March 2013
- Lim, Yan Wei; Schmieder, Robert; Haynes, Matthew
- Journal of Cystic Fibrosis, Vol. 12, Issue 2
Single-cell genomic sequencing using Multiple Displacement Amplification
journal, October 2007
- Lasken, Roger S.
- Current Opinion in Microbiology, Vol. 10, Issue 5
A renaissance for the pioneering 16S rRNA gene
journal, October 2008
- Tringe, Susannah G.; Hugenholtz, Philip
- Current Opinion in Microbiology, Vol. 11, Issue 5
The phenomenon of microbial uncultivability
journal, October 2013
- Epstein, Ss
- Current Opinion in Microbiology, Vol. 16, Issue 5
Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates
journal, September 2011
- Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.
- Journal of Microbiological Methods, Vol. 86, Issue 3
Reprint of “Novel mycoviruses discovered from metatranscriptomics survey of soybean phyllosphere phytobiomes”
journal, July 2016
- Marzano, Shin-Yi Lee; Domier, Leslie L.
- Virus Research, Vol. 219
Raman Activated Cell Ejection for Isolation of Single Cells
journal, October 2013
- Wang, Yun; Ji, Yuetong; Wharfe, Emma S.
- Analytical Chemistry, Vol. 85, Issue 22
Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release
journal, February 2015
- Zhang, Peiran; Ren, Lihui; Zhang, Xu
- Analytical Chemistry, Vol. 87, Issue 4
Stable-isotope probing as a tool in microbial ecology
journal, February 2000
- Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R.
- Nature, Vol. 403, Issue 6770, p. 646-649
Real-time detection of actively metabolizing microbes by redox sensing as applied to methylotroph populations in Lake Washington
journal, March 2008
- Kalyuzhnaya, Marina G.; Lidstrom, Mary E.; Chistoserdova, Ludmila
- The ISME Journal, Vol. 2, Issue 7
A novel fungal family of oligopeptide transporters identified by functional metatranscriptomics of soil eukaryotes
journal, June 2011
- Damon, Coralie; Vallon, Laurent; Zimmermann, Sabine
- The ISME Journal, Vol. 5, Issue 12
De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities
journal, June 2011
- Narasingarao, Priya; Podell, Sheila; Ugalde, Juan A.
- The ISME Journal, Vol. 6, Issue 1
Sizing up metatranscriptomics
journal, August 2012
- Moran, Mary Ann; Satinsky, Brandon; Gifford, Scott M.
- The ISME Journal, Vol. 7, Issue 2
Erratum: Community transcriptomic assembly reveals microbes that contribute to deep-sea carbon and nitrogen cycling
journal, September 2013
- Baker, Brett J.; Sheik, Cody S.; Taylor, Chris A.
- The ISME Journal, Vol. 7, Issue 10
Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses
journal, July 2013
- Blazewicz, Steven J.; Barnard, Romain L.; Daly, Rebecca A.
- The ISME Journal, Vol. 7, Issue 11
Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP
journal, June 2013
- Gutierrez, Tony; Singleton, David R.; Berry, David
- The ISME Journal, Vol. 7, Issue 11
Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics
journal, November 2014
- Hua, Zheng-Shuang; Han, Yu-Jiao; Chen, Lin-Xing
- The ISME Journal, Vol. 9, Issue 6
Metatranscriptomics of N2-fixing cyanobacteria in the Amazon River plume
journal, December 2014
- Hilton, Jason A.; Satinsky, Brandon M.; Doherty, Mary
- The ISME Journal, Vol. 9, Issue 7
Metatranscriptomic insights into polyphosphate metabolism in marine sediments
journal, September 2015
- Jones, Daniel S.; Flood, Beverly E.; Bailey, Jake V.
- The ISME Journal, Vol. 10, Issue 4
The energetics of anabolism in natural settings
journal, February 2016
- LaRowe, Douglas E.; Amend, Jan P.
- The ISME Journal, Vol. 10, Issue 6
Coupled RNA-SIP and metatranscriptomics of active chemolithoautotrophic communities at a deep-sea hydrothermal vent
journal, February 2016
- Fortunato, Caroline S.; Huber, Julie A.
- The ISME Journal, Vol. 10, Issue 8
Archaea predominate among ammonia-oxidizing prokaryotes in soils
journal, August 2006
- Leininger, S.; Urich, T.; Schloter, M.
- Nature, Vol. 442, Issue 7104
Bacterial carbon processing by generalist species in the coastal ocean
journal, January 2008
- Mou, Xiaozhen; Sun, Shulei; Edwards, Robert A.
- Nature, Vol. 451, Issue 7179
Metatranscriptomics reveals unique microbial small RNAs in the ocean’s water column
journal, May 2009
- Shi, Yanmei; Tyson, Gene W.; DeLong, Edward F.
- Nature, Vol. 459, Issue 7244
Validation of two ribosomal RNA removal methods for microbial metatranscriptomics
journal, September 2010
- He, Shaomei; Wurtzel, Omri; Singh, Kanwar
- Nature Methods, Vol. 7, Issue 10
DNA stable-isotope probing
journal, April 2007
- Neufeld, Josh D.; Vohra, Jyotsna; Dumont, Marc G.
- Nature Protocols, Vol. 2, Issue 4
RNA stable-isotope probing
journal, April 2007
- Whiteley, Andrew S.; Thomson, Bruce; Lueders, Tillmann
- Nature Protocols, Vol. 2, Issue 4
Protein-based stable isotope probing
journal, November 2010
- Jehmlich, Nico; Schmidt, Frank; Taubert, Martin
- Nature Protocols, Vol. 5, Issue 12
Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease
journal, December 2016
- Cappello, Valentina; Marchetti, Laura; Parlanti, Paola
- Scientific Reports, Vol. 6, Issue 1
Recombination is a key driver of genomic and phenotypic diversity in a Pseudomonas aeruginosa population during cystic fibrosis infection
journal, January 2015
- Darch, Sophie E.; McNally, Alan; Harrison, Freya
- Scientific Reports, Vol. 5, Issue 1
Structure and activity of multiple nitrifying bacterial populations co-existing in a biofilm
journal, May 2003
- Gieseke, Armin; Bjerrum, Lotte; Wagner, Michael
- Environmental Microbiology, Vol. 5, Issue 5
Scaling up: the next challenge in environmental microbiology
journal, November 2003
- Paerl, Hans W.; Steppe, Timothy F.
- Environmental Microbiology, Vol. 5, Issue 11
Microbial community gene expression in ocean surface waters
journal, March 2008
- Frias-Lopez, J.; Shi, Y.; Tyson, G. W.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 10
Spliced leader-based metatranscriptomic analyses lead to recognition of hidden genomic features in dinoflagellates
journal, November 2010
- Lin, S.; Zhang, H.; Zhuang, Y.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 46
Carbon and nitrogen assimilation in deep subseafloor microbial cells
journal, October 2011
- Morono, Y.; Terada, T.; Nishizawa, M.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 45
Relating the metatranscriptome and metagenome of the human gut
journal, May 2014
- Franzosa, E. A.; Morgan, X. C.; Segata, N.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 22
Microspatial gene expression patterns in the Amazon River Plume
journal, July 2014
- Satinsky, B. M.; Crump, B. C.; Smith, C. B.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 30
Global dispersion and local diversification of the methane seep microbiome
journal, March 2015
- Ruff, S. Emil; Biddle, Jennifer F.; Teske, Andreas P.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 13
Functional group-specific traits drive phytoplankton dynamics in the oligotrophic ocean
journal, October 2015
- Alexander, Harriet; Rouco, Mónica; Haley, Sheean T.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 44
Identification of a complete methane monooxygenase operon from soil by combining stable isotope probing and metagenomic analysis
journal, July 2006
- Dumont, Marc G.; Radajewski, Stefan M.; Miguez, Carlos B.
- Environmental Microbiology, Vol. 8, Issue 7
Raman-FISH: combining stable-isotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function
journal, August 2007
- Huang, Wei E.; Stoecker, Kilian; Griffiths, Robert
- Environmental Microbiology, Vol. 9, Issue 8
Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon
journal, March 2010
- Poretsky, Rachel S.; Sun, Shulei; Mou, Xiaozhen
- Environmental Microbiology, Vol. 12, Issue 3
Drivers of bacterial colonization patterns in stream biofilms
journal, April 2010
- Augspurger, Clemens; Karwautz, Clemens; Mußmann, Marc
- FEMS Microbiology Ecology, Vol. 72, Issue 1
Raman tweezers sorting of single microbial cells
journal, February 2009
- Huang, Wei E.; Ward, Andrew D.; Whiteley, Andrew S.
- Environmental Microbiology Reports, Vol. 1, Issue 1
Resolving Genetic Functions within Microbial Populations: In Situ Analyses Using rRNA and mRNA Stable Isotope Probing Coupled with Single-Cell Raman-Fluorescence In Situ Hybridization
journal, November 2008
- Huang, W. E.; Ferguson, A.; Singer, A. C.
- Applied and Environmental Microbiology, Vol. 75, Issue 1
Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry
journal, March 2007
- Berney, M.; Hammes, F.; Bosshard, F.
- Applied and Environmental Microbiology, Vol. 73, Issue 10
An Uncultivated Nitrate-Reducing Member of the Genus Herminiimonas Degrades Toluene
journal, March 2014
- Kim, So-Jeong; Park, Soo-Je; Jung, Man-Young
- Applied and Environmental Microbiology, Vol. 80, Issue 10
Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride.
journal, January 1996
- Ullrich, S.; Karrasch, B.; Hoppe, H.
- Applied and environmental microbiology, Vol. 62, Issue 12
Distribution of Sulfate-Reducing and Methanogenic Bacteria in Anaerobic Aggregates Determined by Microsensor and Molecular Analyses
journal, October 1999
- Santegoeds, Cecilia M.; Damgaard, Lars Riis; Hesselink, Gijs
- Applied and Environmental Microbiology, Vol. 65, Issue 10
Use of Bromodeoxyuridine Immunocapture To Identify Active Bacteria Associated with Arbuscular Mycorrhizal Hyphae
journal, October 2003
- Artursson, V.; Jansson, J. K.
- Applied and Environmental Microbiology, Vol. 69, Issue 10
Directed Culturing of Microorganisms Using Metatranscriptomics
journal, April 2011
- Bomar, Lindsey; Maltz, Michele; Colston, Sophie
- mBio, Vol. 2, Issue 2
Recovering glycoside hydrolase genes from active tundra cellulolytic bacteria
journal, July 2014
- Pinnell, Lee J.; Dunford, Eric; Ronan, Patrick
- Canadian Journal of Microbiology, Vol. 60, Issue 7
Lack of a type-2 glycosyltransferase in the fish pathogen Flavobacterium psychrophilum determines pleiotropic changes and loss of virulence
journal, January 2015
- Pérez-Pascual, David; Gómez, Esther; Guijarro, José A.
- Veterinary Research, Vol. 46, Issue 1
Human milk microbiota profiles in relation to birthing method, gestation and infant gender
journal, January 2016
- Urbaniak, Camilla; Angelini, Michelle; Gloor, Gregory B.
- Microbiome, Vol. 4, Issue 1
Near-infrared Raman spectroscopy of single optically trapped biological cells
journal, January 2002
- Xie, Changan; Dinno, Mumtaz A.; Li, Yong-qing
- Optics Letters, Vol. 27, Issue 4
Simultaneous Assessment of Soil Microbial Community Structure and Function through Analysis of the Meta-Transcriptome
journal, June 2008
- Urich, Tim; Lanzén, Anders; Qi, Ji
- PLoS ONE, Vol. 3, Issue 6
Differential Growth Responses of Soil Bacterial Taxa to Carbon Substrates of Varying Chemical Recalcitrance
journal, January 2011
- Goldfarb, Katherine C.; Karaoz, Ulas; Hanson, China A.
- Frontiers in Microbiology, Vol. 2
Plant growth in Arabidopsis is assisted by compost soil-derived microbial communities
journal, January 2013
- Carvalhais, Lilia C.; Muzzi, Frederico; Tan, Chin-Hong
- Frontiers in Plant Science, Vol. 4
Gene expression of lactobacilli in murine forestomach biofilms
text, January 2014
- Schwab, Clarissa; Tveit, Alexander T.; Schleper, Christa
- ETH Zurich
Works referencing / citing this record:
Complete nitrification: insights into the ecophysiology of comammox Nitrospira
journal, November 2018
- Koch, Hanna; van Kessel, Maartje A. H. J.; Lücker, Sebastian
- Applied Microbiology and Biotechnology, Vol. 103, Issue 1
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journal, April 2019
- Yin, Xiuran; Wu, Weichao; Maeke, Mara
- The ISME Journal, Vol. 13, Issue 8
Next-generation physiology approaches to study microbiome function at single cell level
journal, February 2020
- Hatzenpichler, Roland; Krukenberg, Viola; Spietz, Rachel L.
- Nature Reviews Microbiology, Vol. 18, Issue 4
Surface-enhanced Raman spectroscopy of microorganisms: limitations and applicability on the single-cell level
journal, January 2019
- Weiss, Ruben; Palatinszky, Márton; Wagner, Michael
- The Analyst, Vol. 144, Issue 3
Visualization is crucial for understanding microbial processes in the ocean
journal, October 2019
- Sebastián, Marta; Gasol, Josep M.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 374, Issue 1786
Microbial conservation in the Anthropocene
journal, May 2018
- Webster, Nicole S.; Wagner, Michael; Negri, Andrew P.
- Environmental Microbiology, Vol. 20, Issue 6
Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients
journal, March 2019
- Funahashi, Kenta; Shiba, Takahiko; Watanabe, Takayasu
- Progress in Orthodontics, Vol. 20, Issue 1
Using Click-Chemistry for Visualizing in Situ Changes of Translational Activity in Planktonic Marine Bacteria
journal, December 2017
- Leizeaga, Ainara; Estrany, Margarita; Forn, Irene
- Frontiers in Microbiology, Vol. 8
Probing the active fraction of soil microbiomes using BONCAT-FACS
journal, June 2019
- Couradeau, Estelle; Sasse, Joelle; Goudeau, Danielle
- Nature Communications, Vol. 10, Issue 1
2038 – When microbes rule the Earth
journal, October 2018
- McGenity, Terry J.
- Environmental Microbiology, Vol. 20, Issue 12
Metabolic dependencies govern microbial syntrophies during methanogenesis in an anaerobic digestion ecosystem
journal, February 2020
- Zhu, Xinyu; Campanaro, Stefano; Treu, Laura
- Microbiome, Vol. 8, Issue 1
Using meta‐omics of contaminated sediments to monitor changes in pathways relevant to climate regulation
journal, December 2018
- Birrer, Simone C.; Dafforn, Katherine A.; Sun, Melanie Y.
- Environmental Microbiology
Elucidation of complexity and prediction of interactions in microbial communities
journal, August 2017
- Zuñiga, Cristal; Zaramela, Livia; Zengler, Karsten
- Microbial Biotechnology, Vol. 10, Issue 6
Complete nitrification: insights into the ecophysiology of comammox Nitrospira
journal, November 2018
- Koch, Hanna; van Kessel, Maartje A. H. J.; Lücker, Sebastian
- Applied Microbiology and Biotechnology, Vol. 103, Issue 1
CO2 conversion to methane and biomass in obligate methylotrophic methanogens in marine sediments
journal, April 2019
- Yin, Xiuran; Wu, Weichao; Maeke, Mara
- The ISME Journal, Vol. 13, Issue 8
Probing the active fraction of soil microbiomes using BONCAT-FACS
journal, June 2019
- Couradeau, Estelle; Sasse, Joelle; Goudeau, Danielle
- Nature Communications, Vol. 10, Issue 1
Visualization is crucial for understanding microbial processes in the ocean
journal, October 2019
- Sebastián, Marta; Gasol, Josep M.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 374, Issue 1786
Elucidation of complexity and prediction of interactions in microbial communities
journal, August 2017
- Zuñiga, Cristal; Zaramela, Livia; Zengler, Karsten
- Microbial Biotechnology, Vol. 10, Issue 6
Current progress and future opportunities in applications of bioinformatics for biodefense and pathogen detection: report from the Winter Mid-Atlantic Microbiome Meet-up, College Park, MD, January 10, 2018
journal, November 2018
- Meisel, Jacquelyn S.; Nasko, Daniel J.; Brubach, Brian
- Microbiome, Vol. 6, Issue 1
Metabolic dependencies govern microbial syntrophies during methanogenesis in an anaerobic digestion ecosystem
journal, February 2020
- Zhu, Xinyu; Campanaro, Stefano; Treu, Laura
- Microbiome, Vol. 8, Issue 1
Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients
journal, March 2019
- Funahashi, Kenta; Shiba, Takahiko; Watanabe, Takayasu
- Progress in Orthodontics, Vol. 20, Issue 1
Ecosystems Monitoring Powered by Environmental Genomics: A Review of Current Strategies with An Implementation Roadmap
preprint, January 2020
- Cordier, Tristan; Sáez, Laura Alonso; Apotheloz-Perret-Gentil, Laure
Using Click-Chemistry for Visualizing in Situ Changes of Translational Activity in Planktonic Marine Bacteria
journal, December 2017
- Leizeaga, Ainara; Estrany, Margarita; Forn, Irene
- Frontiers in Microbiology, Vol. 8