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Title: Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa has at least 21 genes, encoding enzymes that mediate 37 reactions on 24 metabolites, leading to lignin and affecting wood properties. We perturb these 21 pathway genes and integrate transcriptomic, proteomic, fluxomic and phenomic data from 221 lines selected from ~2000 transgenics (6-month-old). The integrative analysis estimates how changing expression of pathway gene or gene combination affects protein abundance, metabolic-flux, metabolite concentrations, and 25 wood traits, including lignin, tree-growth, density, strength, and saccharification. The analysis then predicts improvements in any of these 25 traits individually or in combinations, through engineering expression of specific monolignol genes. The analysis may lead to greater understanding of other pathways for improved growth and adaptation.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [3] ;  [3] ;  [4] ;  [3] ;  [5] ;  [6] ;  [7] ;  [8] ;  [9] ; ORCiD logo [10] ;  [11] ;  [11] ;  [8] ; ORCiD logo [2] ;  [12] more »;  [13] ;  [12] ;  [14] ;  [15] ;  [16] ;  [17] ;  [3] ;  [12] ;  [6] ; ORCiD logo [10] ;  [12] ;  [8] ; ORCiD logo [18] ;  [19] ;  [20] ;  [3] ;  [21] « less
  1. Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
  2. North Carolina State Univ., Raleigh, NC (United States). Electrical and Computer Engineering
  3. North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
  4. Chinese Academy of Forestry, Beijing (China). State Key Lab. of Tree Genetics and Breeding
  5. North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
  6. North Carolina State Univ., Raleigh, NC (United States). Civil, Construction and Environmental Engineering
  7. National Chung-Hsing Univ., Taichung (Taiwan). Dept. of Forestry
  8. North Carolina State Univ., Raleigh, NC (United States). W.M. Keck Fourier Transform Mass Spectrometry Lab., Dept. of Chemistry
  9. National Taiwan Univ., Taipei (Taiwan). School of Forestry and Resource Conservation
  10. Univ. of Wisconsin, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Inst., Dept. of Biochemistry
  11. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
  12. North Carolina State Univ., Raleigh, NC (United States). Dept. of Forest Biomaterials
  13. North Carolina State Univ., Raleigh, NC (United States). Dept. of Operations Research
  14. Beihua Univ., Jilin (China). Dept. of Forestry
  15. Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding
  16. Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; National Taiwan Univ., Taipei (Taiwan). College of Life Science, Dept. of Life Sciences
  17. Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
  18. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
  19. North Carolina State Univ., Raleigh, NC (United States). Bioinformatics Research Center
  20. North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources
  21. Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forest Biomaterials
Publication Date:
Report Number(s):
NREL/JA-2700-71418
Journal ID: ISSN 2041-1723
Grant/Contract Number:
AC36-08GO28308; 31430093; 31470672; 31522014; DBI-0922391; FC02-07ER64494; AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Office of Biological and Environmental Research; National Natural Science Foundation of China (NNSFC); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; genetic engineering; molecular engineering; secondary metabolism
OSTI Identifier:
1435499
Alternate Identifier(s):
OSTI ID: 1460355