skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Quantifying root water extraction after drought recovery using sub-mm in situ empirical data

Abstract

Root-specific responses to stress are not well-known, and have been largely based on indirect measurements of bulk soil water extraction, which limits mechanistic modeling of root function. Here, we used neutron radiography to examine in situ root-soil water dynamics of a previously droughted black cottonwood ( Populus trichocarpa) seedling, contrasting water uptake by younger, thinner or older, thicker parts of the fine root system. The smaller diameter roots had greater water uptake capacity per unit surface area than the larger diameter roots, but they had less total surface area leading to less total water extraction; rates ranged from 0.0027 – 0.0116 g cm -2 hr -1. The finest most-active roots were not visible in the radiographs, indicating the need to include destructive sampling. Analysis based on bulk soil hydraulic properties indicated substantial redistribution of water via saturated/unsaturated flow, capillary wicking, and root hydraulic redistribution across the layers - suggesting water uptake dynamics following an infiltration event may be more complex than approximated by common soil hydraulic or root surface area modeling approaches. Lastly, our results highlight the need for continued exploration of root-trait specific water uptake rates in situ, and impacts of roots on soil hydraulic properties – both criticalmore » components for mechanistic modeling of root function.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Princeton Univ., Princeton, NJ (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS) (SC-27); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1435326
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Plant and Soil
Additional Journal Information:
Journal Volume: 424; Journal Issue: 1-2; Journal ID: ISSN 0032-079X
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Neutron radiography; Modeling; Populus; Hydraulic redistribution; Rhizosphere; Water uptake

Citation Formats

Dhiman, Indu, Bilheux, Hassina Z., DeCarlo, Keito F., Painter, Scott L., Santodonato, Louis J., and Warren, Jeffrey M. Quantifying root water extraction after drought recovery using sub-mm in situ empirical data. United States: N. p., 2017. Web. doi:10.1007/s11104-017-3408-5.
Dhiman, Indu, Bilheux, Hassina Z., DeCarlo, Keito F., Painter, Scott L., Santodonato, Louis J., & Warren, Jeffrey M. Quantifying root water extraction after drought recovery using sub-mm in situ empirical data. United States. doi:10.1007/s11104-017-3408-5.
Dhiman, Indu, Bilheux, Hassina Z., DeCarlo, Keito F., Painter, Scott L., Santodonato, Louis J., and Warren, Jeffrey M. Sat . "Quantifying root water extraction after drought recovery using sub-mm in situ empirical data". United States. doi:10.1007/s11104-017-3408-5. https://www.osti.gov/servlets/purl/1435326.
@article{osti_1435326,
title = {Quantifying root water extraction after drought recovery using sub-mm in situ empirical data},
author = {Dhiman, Indu and Bilheux, Hassina Z. and DeCarlo, Keito F. and Painter, Scott L. and Santodonato, Louis J. and Warren, Jeffrey M.},
abstractNote = {Root-specific responses to stress are not well-known, and have been largely based on indirect measurements of bulk soil water extraction, which limits mechanistic modeling of root function. Here, we used neutron radiography to examine in situ root-soil water dynamics of a previously droughted black cottonwood (Populus trichocarpa) seedling, contrasting water uptake by younger, thinner or older, thicker parts of the fine root system. The smaller diameter roots had greater water uptake capacity per unit surface area than the larger diameter roots, but they had less total surface area leading to less total water extraction; rates ranged from 0.0027 – 0.0116 g cm-2 hr-1. The finest most-active roots were not visible in the radiographs, indicating the need to include destructive sampling. Analysis based on bulk soil hydraulic properties indicated substantial redistribution of water via saturated/unsaturated flow, capillary wicking, and root hydraulic redistribution across the layers - suggesting water uptake dynamics following an infiltration event may be more complex than approximated by common soil hydraulic or root surface area modeling approaches. Lastly, our results highlight the need for continued exploration of root-trait specific water uptake rates in situ, and impacts of roots on soil hydraulic properties – both critical components for mechanistic modeling of root function.},
doi = {10.1007/s11104-017-3408-5},
journal = {Plant and Soil},
number = 1-2,
volume = 424,
place = {United States},
year = {2017},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Root anatomy, morphology, and longevity among root orders in Vaccinium corymbosum (Ericaceae)
journal, December 2008

  • Valenzuela-Estrada, Luis R.; Vera-Caraballo, Vivianette; Ruth, Leah E.
  • American Journal of Botany, Vol. 95, Issue 12
  • DOI: 10.3732/ajb.0800092

Effects of plant roots on soil-water retention and induced suction in vegetated soil
journal, July 2015


Neutron radiography as a tool for revealing root development in soil: capabilities and limitations
journal, December 2008


Beyond the Roots of Young Seedlings: The Influence of Age and Order on Fine Root Physiology
journal, December 2002

  • Wells, Christina E.; Eissenstat, David M.
  • Journal of Plant Growth Regulation, Vol. 21, Issue 4
  • DOI: 10.1007/s00344-003-0011-1

Dynamics of soil water content in the rhizosphere
journal, January 2010


iMARS (iMaging Analysis Research Software)
journal, January 2015


A root is a root is a root? Water uptake rates of Citrus root orders: Water flux by root orders
journal, September 2010


Quantification and Modeling of Local Root Water Uptake Using Neutron Radiography and Deuterated Water
journal, January 2012

  • Zarebanadkouki, M.; Kim, Y. X.; Moradi, A. B.
  • Vadose Zone Journal, Vol. 11, Issue 3
  • DOI: 10.2136/vzj2011.0196

Reply to: Comment on ‘neutron imaging reveals internal plant water dynamics’
journal, August 2013


Absorption of Water and 32 P through Suberized and Unsuberized Roots of Loblolly Pine
journal, June 1975

  • Chung, Hsu-Ho; Kramer, Paul J.
  • Canadian Journal of Forest Research, Vol. 5, Issue 2
  • DOI: 10.1139/x75-031

Redefining fine roots improves understanding of below-ground contributions to terrestrial biosphere processes
journal, March 2015

  • McCormack, M. Luke; Dickie, Ian A.; Eissenstat, David M.
  • New Phytologist, Vol. 207, Issue 3
  • DOI: 10.1111/nph.13363

Quantification of root water uptake in soil using X-ray computed tomography and image-based modelling: Quantification of root water uptake in soil
journal, July 2017

  • Daly, Keith R.; Tracy, Saoirse R.; Crout, Neil M. J.
  • Plant, Cell & Environment, Vol. 41, Issue 1
  • DOI: 10.1111/pce.12983

How the Rhizosphere May Favor Water Availability to Roots
journal, January 2011

  • Carminati, Andrea; Schneider, Christoph L.; Moradi, Ahmad B.
  • Vadose Zone Journal, Vol. 10, Issue 3
  • DOI: 10.2136/vzj2010.0113

Modeling Root Water Uptake in Hydrological and Climate Models
journal, December 2001


Role of aquaporin activity in regulating deep and shallow root hydraulic conductance during extreme drought
journal, May 2014


The Relationship between Transpiration, Root Water Uptake, and Leaf Water Potential
journal, January 1979

  • Aston, M. J.; Lawlor, D. W.
  • Journal of Experimental Botany, Vol. 30, Issue 1
  • DOI: 10.1093/jxb/30.1.169

The CG-1D Neutron Imaging Beamline at the Oak Ridge National Laboratory High Flux Isotope Reactor
journal, January 2015


Root Water Uptake: From Three-Dimensional Biophysical Processes to Macroscopic Modeling Approaches
journal, January 2013

  • Javaux, Mathieu; Couvreur, Valentin; Vanderborght, Jan
  • Vadose Zone Journal, Vol. 12, Issue 4
  • DOI: 10.2136/vzj2013.02.0042

The dynamic role of root-water uptake in coupling potential to actual transpiration
journal, January 2000


Effects of Root-Induced Compaction on Rhizosphere Hydraulic Properties - X-ray Microtomography Imaging and Numerical Simulations
journal, January 2011

  • Aravena, Jazmín E.; Berli, Markus; Ghezzehei, Teamrat A.
  • Environmental Science & Technology, Vol. 45, Issue 2
  • DOI: 10.1021/es102566j

An optimality-based model of the coupled soil moisture and root dynamics
journal, January 2008

  • Schymanski, S. J.; Sivapalan, M.; Roderick, M. L.
  • Hydrology and Earth System Sciences, Vol. 12, Issue 3
  • DOI: 10.5194/hess-12-913-2008

A global Fine-Root Ecology Database to address below-ground challenges in plant ecology
journal, February 2017

  • Iversen, Colleen M.; McCormack, M. Luke; Powell, A. Shafer
  • New Phytologist, Vol. 215, Issue 1, p. 15-26
  • DOI: 10.1111/nph.14486

Challenges in imaging and predictive modeling of rhizosphere processes
journal, April 2016


Root structural and functional dynamics in terrestrial biosphere models - evaluation and recommendations
journal, September 2014

  • Warren, Jeffrey M.; Hanson, Paul J.; Iversen, Colleen M.
  • New Phytologist, Vol. 205, Issue 1
  • DOI: 10.1111/nph.13034

A mathematical model for water and nutrient uptake by plant root systems
journal, May 2004


Water gradient profiles at bean plant roots determined by neutron beam analysis
journal, May 2005


Measurements of water uptake of maize roots: the key function of lateral roots
journal, August 2015

  • Ahmed, Mutez A.; Zarebanadkouki, Mohsen; Kaestner, Anders
  • Plant and Soil, Vol. 398, Issue 1-2
  • DOI: 10.1007/s11104-015-2639-6

Thermal Neutron Computed Tomography of Soil Water and Plant Roots
journal, January 2008

  • Tumlinson, Leanne G.; Liu, Hungyuan; Silk, Wendy K.
  • Soil Science Society of America Journal, Vol. 72, Issue 5
  • DOI: 10.2136/sssaj2007.0302

Multiple pixel-scale soil water retention curves quantified by neutron radiography
journal, March 2014


Where do roots take up water? Neutron radiography of water flow into the roots of transpiring plants growing in soil
journal, May 2013

  • Zarebanadkouki, Mohsen; Kim, Yangmin X.; Carminati, Andrea
  • New Phytologist, Vol. 199, Issue 4
  • DOI: 10.1111/nph.12330

An empirical model for root water uptake
journal, April 2004


Dynamic Aspects of Water Availability to Plants
journal, January 1960


Three-dimensional visualization and quantification of water content in the rhizosphere
journal, August 2011


Globally optimal stitching of tiled 3D microscopic image acquisitions
journal, April 2009


Microsensing of Water Dynamics and Root Distributions in Sandy Soils
journal, January 2008

  • Segal, Eran; Kushnir, Tammar; Mualem, Yechezkel
  • Vadose Zone Journal, Vol. 7, Issue 3
  • DOI: 10.2136/vzj2007.0121

Observation of a water-depletion region surrounding loblolly pine roots by magnetic resonance imaging.
journal, February 1990

  • MacFall, J. S.; Johnson, G. A.; Kramer, P. J.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 3
  • DOI: 10.1073/pnas.87.3.1203

Efficacy of drainage trenches to stabilise deep slow landslides in clay slopes
journal, March 2016


Mycorrhizal Fungi: Highways for Water and Nutrients in Arid Soils
journal, January 2007


Fine root Architecture of nine North American Trees
journal, May 2002


Do roots mind the gap?
journal, October 2012


Non-uniform soil water extaction by plant roots
journal, January 1989

  • Hainsworth, J. M.; Aylmore, L. A. G.
  • Plant and Soil, Vol. 113, Issue 1
  • DOI: 10.1007/BF02181929

Use of a Three-Dimensional Detailed Modeling Approach for Predicting Root Water Uptake
journal, January 2008

  • Javaux, Mathieu; Schröder, Tom; Vanderborght, Jan
  • Vadose Zone Journal, Vol. 7, Issue 3
  • DOI: 10.2136/vzj2007.0115

Hydraulic conductivity of the root-soil interface of lupin in sandy soil after drying and rewetting
journal, September 2015

  • Zarebanadkouki, Mohsen; Ahmed, Mutez A.; Carminati, Andrea
  • Plant and Soil, Vol. 398, Issue 1-2
  • DOI: 10.1007/s11104-015-2668-1

Neutron radiography and tomography of water distribution in the root zone
journal, May 2010

  • Esser, Hanna G.; Carminati, Andrea; Vontobel, Peter
  • Journal of Plant Nutrition and Soil Science, Vol. 173, Issue 5
  • DOI: 10.1002/jpln.200900188

A pyramid approach to subpixel registration based on intensity
journal, January 1998

  • Thevenaz, P.; Ruttimann, U. E.; Unser, M.
  • IEEE Transactions on Image Processing, Vol. 7, Issue 1
  • DOI: 10.1109/83.650848

Visualization of Root Water Uptake: Quantification of Deuterated Water Transport in Roots Using Neutron Radiography and Numerical Modeling
journal, September 2014

  • Zarebanadkouki, M.; Kroener, E.; Kaestner, A.
  • PLANT PHYSIOLOGY, Vol. 166, Issue 2
  • DOI: 10.1104/pp.114.243212

Neutron imaging reveals internal plant water dynamics
journal, January 2013


Quantitative Imaging of Infiltration, Root Growth, and Root Water Uptake via Neutron Radiography
journal, January 2008

  • Oswald, Sascha E.; Menon, Manoj; Carminati, Andrea
  • Vadose Zone Journal, Vol. 7, Issue 3
  • DOI: 10.2136/vzj2007.0156