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Title: Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343

Journal Article · · Journal of Proteome Research
 [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4];  [1];  [2]; ORCiD logo [1]; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Shull Wollan Center - a Joint Institute for Neutron Sciences, Oak Ridge, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States)
+ Show Author Affiliations

Here, indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoea sp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoea sp. YR343 grown in the presence of tryptophan or IAA. Exposure to IAA resulted in upregulation of proteins predicted to function in carbohydrate and amino acid transport and exopolysaccharide (EPS) biosynthesis. Metabolite profiles of wild-type cells showed the production of IPA, IAA, and tryptophol, consistent with an active IPA pathway. Finally, we constructed an ΔipdC mutant that showed the elimination of tryptophol, consistent with a loss of IpdC activity, but was still able to produce IAA (20% of wild-type levels). Although we failed to detect intermediates from other known IAA biosynthetic pathways, this result suggests the possibility of an alternate pathway or the production of IAA by a nonenzymatic route in Pantoea sp. YR343. The ΔipdC mutant was able to efficiently colonize poplar, suggesting that an active IPA pathway is not required for plant association.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1435312
Journal Information:
Journal of Proteome Research, Vol. 17, Issue 4; ISSN 1535-3893
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 17 works
Citation information provided by
Web of Science

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Cited By (2)

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos journal January 2018
Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants journal June 2018

Figures / Tables (14)

Figure 1(p. 12)figure Figure 1
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Table 3: Part 4(p. 22)table Table 3: Part 4
Table 3: Part 5(p. 23)table Table 3: Part 5
Figure 2(p. 24)figure Figure 2
Figure 3(p. 27)figure Figure 3
Figure 4(p. 29)figure Figure 4

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59 BASIC BIOLOGICAL SCIENCES
indole-3-acetic acid
indole-3-pyruvate decarboxylase
Pantoea sp. YR343
plant colonization
poplar
tryptophol