Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343

Estenson, Kasey N.; Hurst, Gregory B.; Standaert, Robert F.; Bible, Amber N.; Garcia, David C.; Chourey, Karuna; Doktycz, Mitchel J.; Morrell-Falvey, Jennifer L.

doi:10.1021/acs.jproteome.7b00708

Title: Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343

Abstract

Here, indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoea sp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoea sp. YR343 grown in the presence of tryptophan or IAA. Exposure to IAA resulted in upregulation of proteins predicted to function in carbohydrate and amino acid transport and exopolysaccharide (EPS) biosynthesis. Metabolite profiles of wild-type cells showed the production of IPA, IAA, and tryptophol, consistent with an active IPA pathway. Finally, we constructed an ΔipdC mutant that showed the elimination of tryptophol, consistent with a loss of IpdC activity, but was still able to produce IAA (20% of wild-type levels). Although we failed to detect intermediates from other known IAA biosynthetic pathways, this result suggests the possibility of an alternate pathway or the production of IAA by a nonenzymatic route in Pantoea sp. YR343. The ΔipdC mutant was able to efficiently colonize poplar, suggesting that an active IPA pathway is not required for plant association.

Authors:

Estenson, Kasey N. ^[1];

^[2];

^[3];

^[4]; Garcia, David C. ^[1]; Chourey, Karuna ^[2];

^[1];

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Shull Wollan Center - a Joint Institute for Neutron Sciences, Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)

Publication Date:: 2018-02-21

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1435312

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Proteome Research

Additional Journal Information:: Journal Volume: 17; Journal Issue: 4; Journal ID: ISSN 1535-3893

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; indole-3-acetic acid; indole-3-pyruvate decarboxylase; Pantoea sp. YR343; plant colonization; poplar; tryptophol

Citation Formats


                    Estenson, Kasey N., Hurst, Gregory B., Standaert, Robert F., Bible, Amber N., Garcia, David C., Chourey, Karuna, Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L. Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343.  United States: N. p., 2018. 
Web.  doi:10.1021/acs.jproteome.7b00708.

Copy to clipboard


                    Estenson, Kasey N., Hurst, Gregory B., Standaert, Robert F., Bible, Amber N., Garcia, David C., Chourey, Karuna, Doktycz, Mitchel J., & Morrell-Falvey, Jennifer L. Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343.  United States.  https://doi.org/10.1021/acs.jproteome.7b00708

Copy to clipboard


                    Estenson, Kasey N., Hurst, Gregory B., Standaert, Robert F., Bible, Amber N., Garcia, David C., Chourey, Karuna, Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L. Wed .  
"Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343".  United States.  https://doi.org/10.1021/acs.jproteome.7b00708.  https://www.osti.gov/servlets/purl/1435312.

Copy to clipboard


                    
@article{osti_1435312,

  title        = {Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343},

  author       = {Estenson, Kasey N. and Hurst, Gregory B. and Standaert, Robert F. and Bible, Amber N. and Garcia, David C. and Chourey, Karuna and Doktycz, Mitchel J. and Morrell-Falvey, Jennifer L.},

  abstractNote = {Here, indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoea sp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoea sp. YR343 grown in the presence of tryptophan or IAA. Exposure to IAA resulted in upregulation of proteins predicted to function in carbohydrate and amino acid transport and exopolysaccharide (EPS) biosynthesis. Metabolite profiles of wild-type cells showed the production of IPA, IAA, and tryptophol, consistent with an active IPA pathway. Finally, we constructed an ΔipdC mutant that showed the elimination of tryptophol, consistent with a loss of IpdC activity, but was still able to produce IAA (20% of wild-type levels). Although we failed to detect intermediates from other known IAA biosynthetic pathways, this result suggests the possibility of an alternate pathway or the production of IAA by a nonenzymatic route in Pantoea sp. YR343. The ΔipdC mutant was able to efficiently colonize poplar, suggesting that an active IPA pathway is not required for plant association.},

  doi          = {10.1021/acs.jproteome.7b00708},

  journal      = {Journal of Proteome Research},

  number       = 4,

  volume       = 17,

  place        = {United States},

  year         = {2018},

  month        = {2}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/acs.jproteome.7b00708

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 3 works

Citation information provided by
Web of Science

Figures / Tables:

Figure 1: Tryptophan-dependent IAA biosynthetic pathways in Pantoea sp. YR343. The pathways are color-coded based on genomic analyses with green lines and checks indicating the presence of genes encoding candidate enzymes for each step in the pathway, orange lines and questions marks indicating the presence of possible candidate gene products,more »

All figures and tables (14 total)

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Distinct Microbial Communities within the Endosphere and Rhizosphere of Populus deltoides Roots across Contrasting Soil Types
journal, July 2011

Gottel, Neil R.; Castro, Hector F.; Kerley, Marilyn
Applied and Environmental Microbiology, Vol. 77, Issue 17
DOI: 10.1128/AEM.05255-11

A Multifactor Analysis of Fungal and Bacterial Community Structure in the Root Microbiome of Mature Populus deltoides Trees
journal, October 2013

Shakya, Migun; Gottel, Neil; Castro, Hector
PLoS ONE, Vol. 8, Issue 10
DOI: 10.1371/journal.pone.0076382

Aromatic Amino Acid-Dependent Expression of Indole-3-Pyruvate Decarboxylase Is Regulated by TyrR in Enterobacter cloacae UW5
journal, November 2008

Ryu, R. Julie; Patten, Cheryl L.
Journal of Bacteriology, Vol. 190, Issue 21
DOI: 10.1128/JB.00804-08

Transcriptional Analysis of the Azospirillum brasilense Indole-3-Pyruvate Decarboxylase Gene and Identification of a cis -Acting Sequence Involved in Auxin Responsive Expression
journal, April 2005

Broek, A. Vande; Gysegom, P.; Ona, O.
Molecular Plant-Microbe Interactions®, Vol. 18, Issue 4
DOI: 10.1094/MPMI-18-0311

Identification and Isolation of the Indole-3-Pyruvate Decarboxylase Gene from Azospirillum brasilense Sp7: Sequencing and Functional Analysis of the Gene Locus
journal, June 1998

Zimmer, Wolfgang; Wesche, Markus; Timmermans, Line
Current Microbiology, Vol. 36, Issue 6
DOI: 10.1007/s002849900317

Transcriptome Analysis of the Rhizosphere Bacterium Azospirillum brasilense Reveals an Extensive Auxin Response
journal, February 2011

Van Puyvelde, Sandra; Cloots, Lore; Engelen, Kristof
Microbial Ecology, Vol. 61, Issue 4
DOI: 10.1007/s00248-011-9819-6

Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense
journal, November 2000

Carreño-Lopez, R.; Campos-Reales, N.; Elmerich, C.
Molecular Genetics and Genomics, Vol. 264, Issue 4
DOI: 10.1007/s004380000340

Auxins Upregulate Expression of the Indole-3-Pyruvate Decarboxylase Gene in Azospirillum brasilense
journal, February 1999

Vande Broek, Ann; Lambrecht, Mark; Eggermont, Kristel
Journal of Bacteriology, Vol. 181, Issue 4
DOI: 10.1128/JB.181.4.1338-1342.1999

Organization of the ipdC region regulates IAA levels in different Azospirillum brasilense strains: molecular and functional analysis of ipdC in strain SM
journal, May 2008

Malhotra, Mandira; Srivastava, Sheela
Environmental Microbiology, Vol. 10, Issue 5
DOI: 10.1111/j.1462-2920.2007.01529.x

Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus
journal, July 2012

Brady, Carrie L.; Cleenwerck, Ilse; van der Westhuizen, Lorinda
International Journal of Systematic and Evolutionary Microbiology, Vol. 62, Issue Pt_7
DOI: 10.1099/ijs.0.032615-0

A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
journal, April 2016

Bible, Amber N.; Fletcher, Sarah J.; Pelletier, Dale A.
Frontiers in Microbiology, Vol. 7
DOI: 10.3389/fmicb.2016.00491

NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
Nature Methods, Vol. 9, Issue 7
DOI: 10.1038/nmeth.2089

Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides
journal, October 2012

Brown, S. D.; Utturkar, S. M.; Klingeman, D. M.
Journal of Bacteriology, Vol. 194, Issue 21
DOI: 10.1128/JB.01243-12

IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013

Markowitz, Victor M.; Chen, I-Min A.; Palaniappan, Krishna
Nucleic Acids Research, Vol. 42, Issue D1
DOI: 10.1093/nar/gkt963

Molecular cloning and sequence analysis of an Azospirilium brasilense indole-3-pyruvate decarboxylase gene
journal, July 1994

Costacurta, Antonia; Keijers, Veerle; Vanderleyden, Jos
Molecular and General Genetics MGG, Vol. 243, Issue 4
DOI: 10.1007/BF00280477

Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1
journal, December 2013

Romasi, Elisa Friska; Lee, Jinho
Journal of Microbiology and Biotechnology, Vol. 23, Issue 12
DOI: 10.4014/jmb.1308.08082

Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB ₂ ABC Transporter in Escherichia coli
journal, February 2009

Rice, Christopher D.; Pollard, Jacob E.; Lewis, Zachery T.
Applied and Environmental Microbiology, Vol. 75, Issue 3
DOI: 10.1128/AEM.01046-08

Contribution of Indole-3-Acetic Acid Production to the Epiphytic Fitness of Erwinia herbicola
journal, September 1998

Brandl, M. T.; Lindow, S. E.
Applied and Environmental Microbiology, Vol. 64, Issue 9
DOI: 10.1128/AEM.64.9.3256-3263.1998

Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation
journal, March 2007

Perrig, D.; Boiero, M. L.; Masciarelli, O. A.
Applied Microbiology and Biotechnology, Vol. 75, Issue 5
DOI: 10.1007/s00253-007-0909-9

Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings
journal, April 1967

Rayle, David L.; Purves, William K.
Plant Physiology, Vol. 42, Issue 4
DOI: 10.1104/pp.42.4.520

Indole-3-ethanol Oxidase: Kinetics, Inhibition, and Regulation by Auxins
journal, April 1973

Percival, Frank W.; Purves, William K.; Vickery, Larry E.
Plant Physiology, Vol. 51, Issue 4
DOI: 10.1104/pp.51.4.739

Works referencing / citing this record:

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos
journal, January 2018

Silva, Saúl; Ascenso, Osvaldo S.; Lourenço, Eva C.
Organic & Biomolecular Chemistry, Vol. 16, Issue 38
DOI: 10.1039/c8ob02096e

Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants
journal, June 2018

Veach, Allison M.; Yip, Daniel; Engle, Nancy L.
Plant and Soil, Vol. 429, Issue 1-2
DOI: 10.1007/s11104-018-3692-8

Figures / Tables found in this record:

Figure 1 (p. 12)

Table 1: Part 1 (p. 13)

Table 1: Part 2 (p. 14)

Table 1: Part 3 (p. 15)

Table 2: Part 1 (p. 16)

Table 2: Part 2 (p. 17)

Table 3: Part 1 (p. 19)

Table 3: Part 2 (p. 20)

Table 3: Part 3 (p. 21)

Table 3: Part 4 (p. 22)

Table 3: Part 5 (p. 23)

Figure 2 (p. 24)

Figure 3 (p. 27)

Figure 4 (p. 29)

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

Similar Records in DOE PAGES and OSTI.GOV collections:

Computationally Guided Discovery and Experimental Validation of Indole-3-acetic Acid Synthesis Pathways

Journal Article Garcia, David C. ; Cheng, Xiaolin ; Land, Miriam L. ; ... - ACS Chemical Biology

Elucidating the interaction networks associated with secondary metabolite production in microorganisms is an ongoing challenge made all the more daunting by the rate at which DNA sequencing technology reveals new genes and potential pathways. Developing the culturing methods, expression conditions, and genetic systems needed for validating pathways in newly discovered microorganisms is often not possible. Therefore, new tools and techniques are needed for defining complex metabolic pathways. Here, we describe an in vitro computationally assisted pathway description approach that employs bioinformatic searches of genome databases, protein structural modeling, and protein–ligand-docking simulations to predict the gene products most likely to bemore »« less
Cited by 1
https://doi.org/10.1021/acschembio.9b00725

Full Text Available
Loss of carotenoids from membranes of Pantoea sp. YR343 results in altered lipid composition and changes in membrane biophysical properties

Journal Article Kumar, Sushmitha Vijaya ; Taylor, Graham ; Hasim, Sahar ; ... - Biochimica et Biophysica Acta. Biomembranes

Bacterial membranes are complex mixtures of lipids and proteins, the combination of which confers biophysical properties that allows cells to respond to environmental conditions. Carotenoids are sterol analogs that are important for regulating membrane dynamics. The membrane of Pantoea sp. YR343 is characterized by the presence of the carotenoid zeaxanthin, and a carotenoid-deficient mutant, ΔcrtB, displays defects in root colonization, reduced secretion of indole-3-acetic acid, and defects in biofilm formation. Here we demonstrate that the loss of carotenoids results in changes to the membrane lipid composition in Pantoea sp. YR343, including increased amounts of unsaturated fatty acids in the ΔcrtBmore »« less
Cited by 1
https://doi.org/10.1016/j.bbamem.2019.05.009
A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome

Journal Article Vijaya Kumar, Sushmitha ; Abraham, Paul E. ; Hurst, Gregory B. ; ... - Scientific Reports

Abstract Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, usingmore »« less
https://doi.org/10.1038/s41598-020-71672-w
Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343

Journal Article Bible, Amber N. ; Chang, Mang ; Morrell-Falvey, Jennifer L. ; ... - PLoS ONE

Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides , forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. In this work, we identified three diguanylate cyclases in the plant-associated microbe Pantoea sp. YR343 that are expressed in the presence of plant roots. One of these diguanylate cyclases, DGC2884, localizes to discrete sites in the cellsmore »« less
https://doi.org/10.1371/journal.pone.0248607
A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

Journal Article Bible, Amber ; Fletcher, Sarah J ; Pelletier, Dale A ; ... - Frontiers in Microbiology

The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically-important plants. Pantoea sp. YR₃₄₃ is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid. Pantoea sp. YR₃₄₃ readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. Tomore »« less
Cited by 2
https://doi.org/10.3389/fmicb.2016.00491

Full Text Available

Similar Records

Title: Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343

Abstract

Citation Formats

Figures / Tables:

Distinct Microbial Communities within the Endosphere and Rhizosphere of Populus deltoides Roots across Contrasting Soil Types journal, July 2011

A Multifactor Analysis of Fungal and Bacterial Community Structure in the Root Microbiome of Mature Populus deltoides Trees journal, October 2013

Aromatic Amino Acid-Dependent Expression of Indole-3-Pyruvate Decarboxylase Is Regulated by TyrR in Enterobacter cloacae UW5 journal, November 2008

Transcriptional Analysis of the Azospirillum brasilense Indole-3-Pyruvate Decarboxylase Gene and Identification of a cis -Acting Sequence Involved in Auxin Responsive Expression journal, April 2005

Identification and Isolation of the Indole-3-Pyruvate Decarboxylase Gene from Azospirillum brasilense Sp7: Sequencing and Functional Analysis of the Gene Locus journal, June 1998

Transcriptome Analysis of the Rhizosphere Bacterium Azospirillum brasilense Reveals an Extensive Auxin Response journal, February 2011

Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense journal, November 2000

Auxins Upregulate Expression of the Indole-3-Pyruvate Decarboxylase Gene in Azospirillum brasilense journal, February 1999

Organization of the ipdC region regulates IAA levels in different Azospirillum brasilense strains: molecular and functional analysis of ipdC in strain SM journal, May 2008

Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus journal, July 2012

A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization journal, April 2016

NIH Image to ImageJ: 25 years of image analysis journal, June 2012

Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides journal, October 2012

IMG 4 version of the integrated microbial genomes comparative analysis system journal, October 2013

Molecular cloning and sequence analysis of an Azospirilium brasilense indole-3-pyruvate decarboxylase gene journal, July 1994

Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1 journal, December 2013

Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB 2 ABC Transporter in Escherichia coli journal, February 2009

Contribution of Indole-3-Acetic Acid Production to the Epiphytic Fitness of Erwinia herbicola journal, September 1998

Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation journal, March 2007

Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings journal, April 1967

Indole-3-ethanol Oxidase: Kinetics, Inhibition, and Regulation by Auxins journal, April 1973

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos journal, January 2018

Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants journal, June 2018

Distinct Microbial Communities within the Endosphere and Rhizosphere of Populus deltoides Roots across Contrasting Soil Types
journal, July 2011

A Multifactor Analysis of Fungal and Bacterial Community Structure in the Root Microbiome of Mature Populus deltoides Trees
journal, October 2013

Aromatic Amino Acid-Dependent Expression of Indole-3-Pyruvate Decarboxylase Is Regulated by TyrR in Enterobacter cloacae UW5
journal, November 2008

Transcriptional Analysis of the Azospirillum brasilense Indole-3-Pyruvate Decarboxylase Gene and Identification of a cis -Acting Sequence Involved in Auxin Responsive Expression
journal, April 2005

Identification and Isolation of the Indole-3-Pyruvate Decarboxylase Gene from Azospirillum brasilense Sp7: Sequencing and Functional Analysis of the Gene Locus
journal, June 1998

Transcriptome Analysis of the Rhizosphere Bacterium Azospirillum brasilense Reveals an Extensive Auxin Response
journal, February 2011

Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense
journal, November 2000

Auxins Upregulate Expression of the Indole-3-Pyruvate Decarboxylase Gene in Azospirillum brasilense
journal, February 1999

Organization of the ipdC region regulates IAA levels in different Azospirillum brasilense strains: molecular and functional analysis of ipdC in strain SM
journal, May 2008

Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus
journal, July 2012

A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
journal, April 2016

NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides
journal, October 2012

IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013

Molecular cloning and sequence analysis of an Azospirilium brasilense indole-3-pyruvate decarboxylase gene
journal, July 1994

Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1
journal, December 2013

Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB ₂ ABC Transporter in Escherichia coli
journal, February 2009

Contribution of Indole-3-Acetic Acid Production to the Epiphytic Fitness of Erwinia herbicola
journal, September 1998

Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation
journal, March 2007

Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings
journal, April 1967

Indole-3-ethanol Oxidase: Kinetics, Inhibition, and Regulation by Auxins
journal, April 1973

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos
journal, January 2018

Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants
journal, June 2018