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Title: Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument

Abstract

Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.

Authors:
 [1];  [2];  [3];  [2];  [2]
  1. Univ. of Windsor, ON (Canada)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Pennsylvania State Univ., University Park, PA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1435280
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 51; Journal Issue: 2; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Citation Formats

Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., and Katsaras, John. Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument. United States: N. p., 2018. Web. doi:10.1107/S1600576718001243.
Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., & Katsaras, John. Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument. United States. https://doi.org/10.1107/S1600576718001243
Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., and Katsaras, John. Tue . "Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument". United States. https://doi.org/10.1107/S1600576718001243. https://www.osti.gov/servlets/purl/1435280.
@article{osti_1435280,
title = {Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument},
author = {Marquardt, Drew and Frontzek, Matthias D. and Zhao, Yu and Chakoumakos, Bryan C. and Katsaras, John},
abstractNote = {Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.},
doi = {10.1107/S1600576718001243},
journal = {Journal of Applied Crystallography (Online)},
number = 2,
volume = 51,
place = {United States},
year = {2018},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 6 works
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Figures / Tables:

Figure 1 Figure 1: The Wide-Angle Neutron Diffractometer (WAND) at HFIR. The primary components of the diffractometer are as follows: (1) collimation slit S1; (2) collimation slit S2; (3) motorized slit package (maximum 9.4 mm horizontal, 49.4 mm vertical); (4) sample environment; (5) thermocouple; (6) goniometer stage for sample alignment; (7) 640-more » channel detector; and (8) oscillating radial collimator.« less

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