Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument
Abstract
Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.
- Authors:
-
- Univ. of Windsor, ON (Canada)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Pennsylvania State Univ., University Park, PA (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1435280
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Applied Crystallography (Online)
- Additional Journal Information:
- Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 51; Journal Issue: 2; Journal ID: ISSN 1600-5767
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
Citation Formats
Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., and Katsaras, John. Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument. United States: N. p., 2018.
Web. doi:10.1107/S1600576718001243.
Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., & Katsaras, John. Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument. United States. https://doi.org/10.1107/S1600576718001243
Marquardt, Drew, Frontzek, Matthias D., Zhao, Yu, Chakoumakos, Bryan C., and Katsaras, John. Tue .
"Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument". United States. https://doi.org/10.1107/S1600576718001243. https://www.osti.gov/servlets/purl/1435280.
@article{osti_1435280,
title = {Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument},
author = {Marquardt, Drew and Frontzek, Matthias D. and Zhao, Yu and Chakoumakos, Bryan C. and Katsaras, John},
abstractNote = {Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.},
doi = {10.1107/S1600576718001243},
journal = {Journal of Applied Crystallography (Online)},
number = 2,
volume = 51,
place = {United States},
year = {Tue Feb 06 00:00:00 EST 2018},
month = {Tue Feb 06 00:00:00 EST 2018}
}
Web of Science
Figures / Tables:
Works referenced in this record:
The Observation of Highly Ordered Domains in Membranes with Cholesterol
journal, June 2013
- Armstrong, Clare L.; Marquardt, Drew; Dies, Hannah
- PLoS ONE, Vol. 8, Issue 6
Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles
journal, February 2017
- Eicher, Barbara; Heberle, Frederick A.; Marquardt, Drew
- Journal of Applied Crystallography, Vol. 50, Issue 2
Accuracy of Determination of Position and Width of Molecular Groups in Biological and Lipid Membranes via Neutron Diffraction
journal, July 1997
- Gordeliy, V. I.; Chernov, N. I.
- Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 4
Cholesterol Hydroxyl Group Is Found To Reside in the Center of a Polyunsaturated Lipid Membrane
journal, January 2006
- Harroun, Thad A.; Katsaras, John; Wassall, Stephen R.
- Biochemistry, Vol. 45, Issue 4
Cholesterol Is Found To Reside in the Center of a Polyunsaturated Lipid Membrane
journal, July 2008
- Harroun, Thad A.; Katsaras, John; Wassall, Stephen R.
- Biochemistry, Vol. 47, Issue 27
Neutron and X-ray scattering for biophysics and biotechnology: examples of self-assembled lipid systems
journal, January 2009
- Harroun, Thad A.; Kučerka, Norbert; Nieh, Mu-Ping
- Soft Matter, Vol. 5, Issue 14
The suite of small-angle neutron scattering instruments at Oak Ridge National Laboratory
journal, February 2018
- Heller, William T.; Cuneo, Matthew; Debeer-Schmitt, Lisa
- Journal of Applied Crystallography, Vol. 51, Issue 2
Sunflower lecithin and possibilities for utilization
journal, October 1993
- Holló, J.; Perédi, J.; Ruzics, A.
- Journal of the American Oil Chemists’ Society, Vol. 70, Issue 10
Evidence for a Two-Dimensional Molecular Lattice in Subgel Phase DPPC Bilayers
journal, April 1995
- Katsaras, J.; Raghunathan, V. A.; Dufourc, E. J.
- Biochemistry, Vol. 34, Issue 14
Clarification of the ripple phase of lecithin bilayers using fully hydrated, aligned samples
journal, May 2000
- Katsaras, J.; Tristram-Nagle, S.; Liu, Y.
- Physical Review E, Vol. 61, Issue 5
Location of chlorhexidine in DMPC model membranes: a neutron diffraction study
journal, June 2010
- Komljenović, Ivana; Marquardt, Drew; Harroun, Thad A.
- Chemistry and Physics of Lipids, Vol. 163, Issue 6
Structure and hydration of membranes embedded with voltage-sensing domains
journal, November 2009
- Krepkiy, Dmitriy; Mihailescu, Mihaela; Freites, J. Alfredo
- Nature, Vol. 462, Issue 7272
Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry
journal, March 2015
- Krüger, Stephanie; Bürmann, Laura; Morlock, Gertrud E.
- Journal of Agricultural and Food Chemistry, Vol. 63, Issue 11
Cholesterol in Bilayers with PUFA Chains: Doping with DMPC or POPC Results in Sterol Reorientation and Membrane-Domain Formation
journal, September 2010
- Kučerka, Norbert; Marquardt, Drew; Harroun, Thad A.
- Biochemistry, Vol. 49, Issue 35
The Functional Significance of Lipid Diversity: Orientation of Cholesterol in Bilayers Is Determined by Lipid Species
journal, November 2009
- Kučerka, Norbert; Marquardt, Drew; Harroun, Thad A.
- Journal of the American Chemical Society, Vol. 131, Issue 45
What determines the thickness of a biological membrane
journal, January 2009
- Kucerka, N.; Nieh, M.; Pencer, J.
- General Physiology and Biophysics, Vol. 28, Issue 2
α-Tocopherol Is Well Designed to Protect Polyunsaturated Phospholipids: MD Simulations
journal, October 2015
- Leng, Xiaoling; Kinnun, Jacob J.; Marquardt, Drew
- Biophysical Journal, Vol. 109, Issue 8
Neutron Scattering at the Intersection of Heart Health Science and Biophysics
journal, June 2015
- Marquardt, Drew; Alsop, Richard; Rheinstädter, Maikel
- Journal of Cardiovascular Development and Disease, Vol. 2, Issue 2
Dimyristoyl Phosphatidylcholine: A Remarkable Exception to α-Tocopherol’s Membrane Presence
journal, December 2013
- Marquardt, Drew; Williams, Justin A.; Kinnun, Jacob J.
- Journal of the American Chemical Society, Vol. 136, Issue 1
Tocopherol Activity Correlates with Its Location in a Membrane: A New Perspective on the Antioxidant Vitamin E
journal, May 2013
- Marquardt, Drew; Williams, Justin A.; Kučerka, Norbert
- Journal of the American Chemical Society, Vol. 135, Issue 20
Acyl-Chain Methyl Distributions of Liquid-Ordered and -Disordered Membranes
journal, March 2011
- Mihailescu, Mihaela; Vaswani, Rishi G.; Jardón-Valadez, Eduardo
- Biophysical Journal, Vol. 100, Issue 6
The Control of Humidity by Saturated Salt Solutions
journal, March 1948
- O'Brien, F. E. M.
- Journal of Scientific Instruments, Vol. 25, Issue 3
Fatty Acids Composition of Vegetable Oils and Its Contribution to Dietary Energy Intake and Dependence of Cardiovascular Mortality on Dietary Intake of Fatty Acids
journal, June 2015
- Orsavova, Jana; Misurcova, Ladislava; Ambrozova, Jarmila
- International Journal of Molecular Sciences, Vol. 16, Issue 12
Time-of-flight Bragg scattering from aligned stacks of lipid bilayers using the Liquids Reflectometer at the Spallation Neutron Source
journal, November 2012
- Pan, Jianjun; Heberle, Frederick A.; Carmichael, Justin R.
- Journal of Applied Crystallography, Vol. 45, Issue 6
Structure of the Phase in a Hydrated Lipid Multilamellar System
journal, May 1995
- Raghunathan, V. A.; Katsaras, J.
- Physical Review Letters, Vol. 74, Issue 22
Recent advances in magnetic structure determination by neutron powder diffraction
journal, October 1993
- Rodríguez-Carvajal, Juan
- Physica B: Condensed Matter, Vol. 192, Issue 1-2
X-ray Scattering Studies of Aligned, Stacked Surfactant Membranes
journal, December 1988
- Sirota, E. B.; Smith, G. S.; Safinya, C. R.
- Science, Vol. 242, Issue 4884
Structure of the phases in a hydrated phosphatidylcholine multimembrane
journal, February 1988
- Smith, G. S.; Sirota, E. B.; Safinya, C. R.
- Physical Review Letters, Vol. 60, Issue 9
Neutron Off-Plane Scattering of Aligned Membranes. I. Method of Measurement
journal, August 1998
- Yang, Lin; Harroun, Thad A.; Heller, William T.
- Biophysical Journal, Vol. 75, Issue 2
Supramolecular Structures of Peptide Assemblies in Membranes by Neutron Off-Plane Scattering: Method of Analysis
journal, November 1999
- Yang, Lin; Weiss, Thomas M.; Harroun, Thad A.
- Biophysical Journal, Vol. 77, Issue 5
Works referencing / citing this record:
Flexible lipid nanomaterials studied by NMR spectroscopy
journal, January 2019
- Mallikarjunaiah, K. J.; Kinnun, Jacob J.; Petrache, Horia I.
- Physical Chemistry Chemical Physics, Vol. 21, Issue 34
A suite-level review of the neutron single-crystal diffraction instruments at Oak Ridge National Laboratory
journal, September 2018
- Coates, L.; Cao, H. B.; Chakoumakos, B. C.
- Review of Scientific Instruments, Vol. 89, Issue 9
WAND 2 —A versatile wide angle neutron powder/single crystal diffractometer
journal, September 2018
- Frontzek, M. D.; Whitfield, R.; Andrews, K. M.
- Review of Scientific Instruments, Vol. 89, Issue 9
A suite-level review of the neutron powder diffraction instruments at Oak Ridge National Laboratory
journal, September 2018
- Calder, S.; An, K.; Boehler, R.
- Review of Scientific Instruments, Vol. 89, Issue 9
Probing orientation information using 3-dimensional reciprocal space volume analysis
journal, January 2019
- Fancher, C. M.; Hoffmann, C. M.; Frontzek, M. D.
- Review of Scientific Instruments, Vol. 90, Issue 1
Foreword to the special issue on advanced neutron scattering instrumentation
journal, June 2018
- Argyriou, Dimitri N.; Allen, Andrew J.
- Journal of Applied Crystallography, Vol. 51, Issue 3