skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Abstract

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibitionmore » of S. aureus growth.« less

Authors:
 [1];  [2];  [3];  [2];  [4];  [5]; ORCiD logo [5];  [6];  [7];  [3]; ORCiD logo [8]
  1. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine. Dept. of Ophthalmology and Visual Sciences
  2. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering
  3. Univ. of Edinburgh, Scotland (United Kingdom). Inst. for Bioengineering
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  6. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Molecular Microbiology and Immunology. Dept. of Surgery
  7. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine
  8. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering; Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Surgery
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Advanced Manufacturing Office (EE-5A); USDOE Office of Science (SC)
OSTI Identifier:
1435225
Grant/Contract Number:  
[AC05-00OR22725]
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Biomaterials Applications
Additional Journal Information:
[ Journal Volume: 33; Journal Issue: 1]; Journal ID: ISSN 0885-3282
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ZnS particles; anti-biofilm; Staphylococcus aureus; anti-MRSA; cryomilling

Citation Formats

Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., and Rivero, Iris V. Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds. United States: N. p., 2018. Web. doi:10.1177/0885328218770530.
Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., & Rivero, Iris V. Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds. United States. doi:10.1177/0885328218770530.
Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., and Rivero, Iris V. Mon . "Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds". United States. doi:10.1177/0885328218770530. https://www.osti.gov/servlets/purl/1435225.
@article{osti_1435225,
title = {Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds},
author = {Tran, Phat L. and Li, Jianqiang and Lungaro, Lisa and Ramesh, Srikanthan and Ivanov, Ilia N. and Moon, Ji-Won and Graham, David E. and Hamood, Abdul and Wang, James and Elfick, Alistair P. D. and Rivero, Iris V.},
abstractNote = {Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.},
doi = {10.1177/0885328218770530},
journal = {Journal of Biomaterials Applications},
number = [1],
volume = [33],
place = {United States},
year = {2018},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

Figure 1 Figure 1: SEM images of (a) original freeze-dried ZnS particles (CompA), with diameters of around 2.8 mm and standard deviation of 1.5 mm and (b) cryomilled ZnS particles (CompB) with average diameter of 0.8 μm and standard deviation of 0.4 μm.

Save / Share:

Works referenced in this record:

Global trends in emerging infectious diseases
journal, February 2008

  • Jones, Kate E.; Patel, Nikkita G.; Levy, Marc A.
  • Nature, Vol. 451, Issue 7181
  • DOI: 10.1038/nature06536

Quorum Sensing and the Social Evolution of Bacterial Virulence
journal, February 2009

  • Rumbaugh, Kendra P.; Diggle, Stephen P.; Watters, Chase M.
  • Current Biology, Vol. 19, Issue 4
  • DOI: 10.1016/j.cub.2009.01.050

Burns, biofilm and a new appraisal of burn wound sepsis
journal, February 2010


Scalable production of microbially mediated zinc sulfide nanoparticles and application to functional thin films
journal, October 2014


Synthesis and mechanical behavior of nanostructured materials via cryomilling
journal, January 2006


Mechanism of Immunologic Suppression in Burn Injury
journal, January 1990


Size-Dependent Bacterial Growth Inhibition and Mechanism of Antibacterial Activity of Zinc Oxide Nanoparticles
journal, April 2011

  • Raghupathi, Krishna R.; Koodali, Ranjit T.; Manna, Adhar C.
  • Langmuir, Vol. 27, Issue 7
  • DOI: 10.1021/la104825u

Mechanisms of Phagocytosis in Macrophages
journal, April 1999


Scalable economic extracellular synthesis of CdS nanostructured particles by a non-pathogenic thermophile
journal, September 2013

  • Moon, Ji-Won; Ivanov, Ilia N.; Duty, Chad E.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 11
  • DOI: 10.1007/s10295-013-1321-3

Effect of Macrolide Consumption on Erythromycin Resistance in Streptococcus pyogenes in Finland in 1997-2001
journal, May 2004

  • Bergman, M.; Huikko, S.; Pihlajamaki, M.
  • Clinical Infectious Diseases, Vol. 38, Issue 9
  • DOI: 10.1086/383309

An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates
journal, March 2011


Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms
journal, April 2002


Calculations of the structural properties of cubic zinc sulfide
journal, April 1990


Fluorescent reporters for Staphylococcus aureus
journal, June 2009

  • Malone, Cheryl L.; Boles, Blaise R.; Lauderdale, Katherine J.
  • Journal of Microbiological Methods, Vol. 77, Issue 3
  • DOI: 10.1016/j.mimet.2009.02.011

Comparative Toxicity of Nanoparticulate ZnO, Bulk ZnO, and ZnCl 2 to a Freshwater Microalga (Pseudokirchneriella subcapitata): The Importance of Particle Solubility
journal, December 2007

  • Franklin, Natasha M.; Rogers, Nicola J.; Apte, Simon C.
  • Environmental Science & Technology, Vol. 41, Issue 24
  • DOI: 10.1021/es071445r

Bacteria and wound healing
journal, January 2004


One-pot synthesis of monodispersed ZnS nanospheres with high antibacterial activity
journal, January 2010

  • Li, Gaiping; Zhai, Junfeng; Li, Dan
  • Journal of Materials Chemistry, Vol. 20, Issue 41
  • DOI: 10.1039/c0jm01776k

Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus
journal, April 2009

  • Tran, P. L.; Hammond, A. A.; Mosley, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 11
  • DOI: 10.1128/AEM.02683-08

Silver against Pseudomonas aeruginosa biofilms
journal, August 2007


An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo
journal, November 2011

  • Tran, Phat L.; Lowry, Nathan; Campbell, Thomas
  • Antimicrobial Agents and Chemotherapy, Vol. 56, Issue 2
  • DOI: 10.1128/AAC.05680-11

The role of Bacteria in burn Mortality
journal, August 1968


Antimicrobial tolerance and the significance of persister cells in recalcitrant chronic wound biofilms: Antimicrobial tolerance in wound biofilms
journal, January 2011


The Different Behavior of Rutile and Anatase Nanoparticles in Forming Oxy Radicals Upon Illumination with Visible Light: An EPR Study
journal, November 2011


Investigation of ZnS Nanoparticle Antibacterial Effect
journal, October 2014


Abrogation of the inflammatory response in LPS-stimulated RAW 264.7 murine macrophages by Zn- and Cu-doped bioactive sol-gel glasses
journal, August 2009

  • Varmette, Elizabeth A.; Nowalk, Jessica R.; Flick, Lisa M.
  • Journal of Biomedical Materials Research Part A, Vol. 90A, Issue 2
  • DOI: 10.1002/jbm.a.32098

Next Science Wound Gel Technology, a Novel Agent That Inhibits Biofilm Development by Gram-Positive and Gram-Negative Wound Pathogens
journal, March 2014

  • Miller, Kyle G.; Tran, Phat L.; Haley, Cecily L.
  • Antimicrobial Agents and Chemotherapy, Vol. 58, Issue 6
  • DOI: 10.1128/AAC.00108-14

Microbiology of the skin and the role of biofilms in infection
journal, October 2011


High-energy ball milling technique for ZnO nanoparticles as antibacterial material
journal, April 2011


The ability of a colloidal silver gel wound dressing to kill bacteria in vitro and in vivo
journal, April 2017


Impact of Silver-Containing Wound Dressings on Bacterial Biofilm Viability and Susceptibility to Antibiotics during Prolonged Treatment
journal, September 2010

  • Kostenko, V.; Lyczak, J.; Turner, K.
  • Antimicrobial Agents and Chemotherapy, Vol. 54, Issue 12
  • DOI: 10.1128/AAC.00825-10

Sol-gel-derived c-axis oriented ZnO thin films
journal, January 1998


Biofilms as Complex Differentiated Communities
journal, October 2002


The lattice relaxation of ZnO single crystal (0001) surface
journal, June 2000


Effect of lattice constant of zinc oxide on antibacterial characteristics
journal, August 2004


Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model
journal, December 2009


Factors Affecting Wound Healing
journal, February 2010


Mechanisms of antibiotic resistance in bacterial biofilms
journal, January 2002


Burn Wound Infections
journal, April 2006