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Title: Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibitionmore » of S. aureus growth.« less
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [5] ; ORCiD logo [5] ;  [6] ;  [7] ;  [3] ; ORCiD logo [8]
  1. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine. Dept. of Ophthalmology and Visual Sciences
  2. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering
  3. Univ. of Edinburgh, Scotland (United Kingdom). Inst. for Bioengineering
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  6. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Molecular Microbiology and Immunology. Dept. of Surgery
  7. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine
  8. Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering; Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Surgery
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Journal of Biomaterials Applications
Additional Journal Information:
Journal Name: Journal of Biomaterials Applications; Journal ID: ISSN 0885-3282
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Advanced Manufacturing Office (EE-5A); USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ZnS particles; anti-biofilm; Staphylococcus aureus; anti-MRSA; cryomilling
OSTI Identifier:
1435225

Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., and Rivero, Iris V.. Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds. United States: N. p., Web. doi:10.1177/0885328218770530.
Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., & Rivero, Iris V.. Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds. United States. doi:10.1177/0885328218770530.
Tran, Phat L., Li, Jianqiang, Lungaro, Lisa, Ramesh, Srikanthan, Ivanov, Ilia N., Moon, Ji-Won, Graham, David E., Hamood, Abdul, Wang, James, Elfick, Alistair P. D., and Rivero, Iris V.. 2018. "Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds". United States. doi:10.1177/0885328218770530.
@article{osti_1435225,
title = {Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds},
author = {Tran, Phat L. and Li, Jianqiang and Lungaro, Lisa and Ramesh, Srikanthan and Ivanov, Ilia N. and Moon, Ji-Won and Graham, David E. and Hamood, Abdul and Wang, James and Elfick, Alistair P. D. and Rivero, Iris V.},
abstractNote = {Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.},
doi = {10.1177/0885328218770530},
journal = {Journal of Biomaterials Applications},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}