Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Tran, Phat L.; Li, Jianqiang; Lungaro, Lisa; Ramesh, Srikanthan; Ivanov, Ilia N.; Moon, Ji-Won; Graham, David E.; Hamood, Abdul; Wang, James; Elfick, Alistair P. D.; Rivero, Iris V.

doi:10.1177/0885328218770530

Title: Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Journal Article · 23 April 2018 · Journal of Biomaterials Applications

DOI: https://doi.org/10.1177/0885328218770530 · OSTI ID:1435225

Tran, Phat L. ^[1]; Li, Jianqiang ^[2]; Lungaro, Lisa ^[3]; Ramesh, Srikanthan ^[2]; Ivanov, Ilia N. ^[4]; Moon, Ji-Won ^[5];

^[5]; Hamood, Abdul ^[6]; Wang, James ^[7]; Elfick, Alistair P. D. ^[3];

^[8]

Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine. Dept. of Ophthalmology and Visual Sciences
Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering
Univ. of Edinburgh, Scotland (United Kingdom). Inst. for Bioengineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Molecular Microbiology and Immunology. Dept. of Surgery
Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). School of Medicine
Iowa State Univ., Ames, IA (United States). Dept. of Industrial and Manufacturing Systems Engineering; Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States). Dept. of Surgery

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This paper examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus (S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Finally, colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office; USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1435225

Journal Information:: Journal of Biomaterials Applications, Vol. 33, Issue 1; ISSN 0885-3282

Country of Publication:: United States

Language:: English

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