skip to main content

DOE PAGESDOE PAGES

Title: Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known. Deletion of the hfsA or B subunits resulted in a roughly 1.8-fold increase in ethanol yield. The increase in ethanol production appears to be associated with an increase in alcohol dehydrogenase activity, which appears to be due, at least in part, to increased expression of the adhE gene, and may suggest a regulatory linkage between hfsB and adhE. We studied this system most intensively in the organism Thermoanaerobacterium saccharolyticum; however, deletion of hfsB also increases ethanol production in other thermophilic bacteria suggesting that this could be used as a general technique for engineering thermophilic bacteria for improved ethanol production in organisms with hfs-type hydrogenases. Since its discovery by Shaw et al. (JAMA 191:6457–64, 2009), the hfs hydrogenase has been suspected to act as a regulator duemore » to the presence of a PAS domain. We provide additional support for the presence of a regulatory phenomenon. In addition, we find a practical application for this scientific insight, namely increasing ethanol yield in strains that are of interest for ethanol production from cellulose or hemicellulose. In two of these organisms (T. xylanolyticum and T. thermosaccharolyticum), the ethanol yields are the highest reported to date.« less
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [4] ;  [5] ;  [6] ;  [2]
  1. Recep Tayyip Erdogan Univ., Rize (Turkey). Dept. of Biology. Molecular Biology Research Lab.
  2. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division. BioEnergy Science Center
  4. Mascoma Corporation, Lebanon, NH (United States)
  5. Karadeniz Technical Univ., Trabzon (Turkey). Dept. of Biology
  6. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering. Dept. of Biological Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
Publication Date:
Grant/Contract Number:
AC05-00OR22725; AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dartmouth College, Hanover, NH (United States); Recep Tayyip Erdogan Univ., Rize (Turkey)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Scientific and Technological Research Council of Turkey
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Thermoanaerobacterium saccharolyticum; Clostridium thermocellum; Thermoanaerobacter mathranii; Thermoanaerobacterium thermosaccharolyticum; Thermoanaerobacterium xylanolyticum; hydrogenase; ethanol
OSTI Identifier:
1435216

Eminoglu, Aysenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Belduz, Ali Osman, Lynd, Lee R., and Olson, Daniel G.. Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria. United States: N. p., Web. doi:10.1186/s13068-017-0968-9.
Eminoglu, Aysenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Belduz, Ali Osman, Lynd, Lee R., & Olson, Daniel G.. Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria. United States. doi:10.1186/s13068-017-0968-9.
Eminoglu, Aysenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Belduz, Ali Osman, Lynd, Lee R., and Olson, Daniel G.. 2017. "Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria". United States. doi:10.1186/s13068-017-0968-9. https://www.osti.gov/servlets/purl/1435216.
@article{osti_1435216,
title = {Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria},
author = {Eminoglu, Aysenur and Murphy, Sean Jean-Loup and Maloney, Marybeth and Lanahan, Anthony and Giannone, Richard J. and Hettich, Robert L. and Tripathi, Shital A. and Belduz, Ali Osman and Lynd, Lee R. and Olson, Daniel G.},
abstractNote = {With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known. Deletion of the hfsA or B subunits resulted in a roughly 1.8-fold increase in ethanol yield. The increase in ethanol production appears to be associated with an increase in alcohol dehydrogenase activity, which appears to be due, at least in part, to increased expression of the adhE gene, and may suggest a regulatory linkage between hfsB and adhE. We studied this system most intensively in the organism Thermoanaerobacterium saccharolyticum; however, deletion of hfsB also increases ethanol production in other thermophilic bacteria suggesting that this could be used as a general technique for engineering thermophilic bacteria for improved ethanol production in organisms with hfs-type hydrogenases. Since its discovery by Shaw et al. (JAMA 191:6457–64, 2009), the hfs hydrogenase has been suspected to act as a regulator due to the presence of a PAS domain. We provide additional support for the presence of a regulatory phenomenon. In addition, we find a practical application for this scientific insight, namely increasing ethanol yield in strains that are of interest for ethanol production from cellulose or hemicellulose. In two of these organisms (T. xylanolyticum and T. thermosaccharolyticum), the ethanol yields are the highest reported to date.},
doi = {10.1186/s13068-017-0968-9},
journal = {Biotechnology for Biofuels},
number = ,
volume = 10,
place = {United States},
year = {2017},
month = {11}
}

Works referenced in this record:

Marker Removal System for Thermoanaerobacterium saccharolyticum and Development of a Markerless Ethanologen
journal, February 2011
  • Shaw, A. Joe; Covalla, Sean; Hogsett, David
  • Applied and Environmental Microbiology, Vol. 77, Issue 7, p. 2534-2536
  • DOI: 10.1128/AEM.01731-10

Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009
  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2