In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
- Sao Paulo State Univ. (UNESP), Aracatuba (Brazil). Faculty of Dentistry of Aracatuba. Dept. of Child and Social Dentistry
- Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Dept. of Molecular Pharmacology and Biological Chemistry
- Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Ann and Robert Lurie Children's Hospital
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States); Sao Paulo State Univ. (UNESP), Aracatuba (Brazil)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Grant/Contract Number:
- AC02-06CH11357
- OSTI ID:
- 1435039
- Journal Information:
- Journal of Applied Oral Science, Vol. 25, Issue 6; ISSN 1678-7757
- Publisher:
- SciELOCopyright Statement
- Country of Publication:
- United States
- Language:
- English
The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion
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journal | January 2019 |
The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion
|
journal | January 2019 |
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