In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Journal Article · 01 December 2017 · Journal of Applied Oral Science

DOI: https://doi.org/10.1590/1678-7757-2016-0513 · OSTI ID:1435039

Vieira, Ana Elisa de Mello ^[1]; Danelon, Marcelle ^[1]; Camara, Danielle Mendes da ^[1]; Rosselli, Eliana Rodrigues ^[1]; Stock, Stuart R. ^[2]; Cannon, Mark L. ^[3]; Xiao, Xianghui ^[4]; De Carlo, Francesco ^[4]; Delbem, Alberto Carlos Botazzo ^[1]

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1. Sao Paulo State Univ. (UNESP), Aracatuba (Brazil). Faculty of Dentistry of Aracatuba. Dept. of Child and Social Dentistry
2. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Dept. of Molecular Pharmacology and Biological Chemistry
3. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Ann and Robert Lurie Children's Hospital
4. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (g_HAp.cm^-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States); Sao Paulo State Univ. (UNESP), Aracatuba (Brazil)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES)
• Grant/Contract Number: AC02-06CH11357
• OSTI ID: 1435039
• Journal Information: Journal of Applied Oral Science, Vol. 25, Issue 6; ISSN 1678-7757
• Publisher: SciELOCopyright Statement
• Country of Publication: United States
• Language: English