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Title: In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (g -3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups formore » %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.« less
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  1. Sao Paulo State Univ. (UNESP), Aracatuba (Brazil). Faculty of Dentistry of Aracatuba. Dept. of Child and Social Dentistry
  2. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Dept. of Molecular Pharmacology and Biological Chemistry
  3. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Ann and Robert Lurie Children's Hospital
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Journal of Applied Oral Science
Additional Journal Information:
Journal Volume: 25; Journal Issue: 6; Journal ID: ISSN 1678-7757
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States); Sao Paulo State Univ. (UNESP), Aracatuba (Brazil)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
60 APPLIED LIFE SCIENCES; X-ray microtomography; tooth remineralization; calcium phosphates; fluoride; dental enamel
OSTI Identifier: