DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Abstract

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoridemore » gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.« less

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [4];  [4];  [1]
  1. Sao Paulo State Univ. (UNESP), Aracatuba (Brazil). Faculty of Dentistry of Aracatuba. Dept. of Child and Social Dentistry
  2. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Dept. of Molecular Pharmacology and Biological Chemistry
  3. Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine. Ann and Robert Lurie Children's Hospital
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); Sao Paulo State Univ. (UNESP), Aracatuba (Brazil)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1435039
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Applied Oral Science
Additional Journal Information:
Journal Volume: 25; Journal Issue: 6; Journal ID: ISSN 1678-7757
Publisher:
SciELO
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; X-ray microtomography; tooth remineralization; calcium phosphates; fluoride; dental enamel

Citation Formats

Vieira, Ana Elisa de Mello, Danelon, Marcelle, Camara, Danielle Mendes da, Rosselli, Eliana Rodrigues, Stock, Stuart R., Cannon, Mark L., Xiao, Xianghui, De Carlo, Francesco, and Delbem, Alberto Carlos Botazzo. In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. United States: N. p., 2017. Web. doi:10.1590/1678-7757-2016-0513.
Vieira, Ana Elisa de Mello, Danelon, Marcelle, Camara, Danielle Mendes da, Rosselli, Eliana Rodrigues, Stock, Stuart R., Cannon, Mark L., Xiao, Xianghui, De Carlo, Francesco, & Delbem, Alberto Carlos Botazzo. In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. United States. https://doi.org/10.1590/1678-7757-2016-0513
Vieira, Ana Elisa de Mello, Danelon, Marcelle, Camara, Danielle Mendes da, Rosselli, Eliana Rodrigues, Stock, Stuart R., Cannon, Mark L., Xiao, Xianghui, De Carlo, Francesco, and Delbem, Alberto Carlos Botazzo. Fri . "In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization". United States. https://doi.org/10.1590/1678-7757-2016-0513. https://www.osti.gov/servlets/purl/1435039.
@article{osti_1435039,
title = {In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization},
author = {Vieira, Ana Elisa de Mello and Danelon, Marcelle and Camara, Danielle Mendes da and Rosselli, Eliana Rodrigues and Stock, Stuart R. and Cannon, Mark L. and Xiao, Xianghui and De Carlo, Francesco and Delbem, Alberto Carlos Botazzo},
abstractNote = {Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.},
doi = {10.1590/1678-7757-2016-0513},
journal = {Journal of Applied Oral Science},
number = 6,
volume = 25,
place = {United States},
year = {2017},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 8 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Comparison of Methods for Evaluating Mineral Loss: Hardness versus Synchrotron Microcomputed Tomography
journal, January 2009

  • Delbem, A. C. B.; Sassaki, K. T.; Vieira, A. E. M.
  • Caries Research, Vol. 43, Issue 5
  • DOI: 10.1159/000231573

The Inhibitory Effect of MI Paste, Fluoride and a Combination of Both on the Progression of Artificial Caries-like Lesions in Enamel
journal, September 2008

  • Pulido, M. T.; Wefel, J. S.; Hernandez, M. M.
  • Operative Dentistry, Vol. 33, Issue 5
  • DOI: 10.2341/07-136

Fluoride Dose Response in pH-Cycling Models Using Bovine Enamel
journal, January 2005

  • de Mello Vieira, Ana Elisa; Botazzo Delbem, Alberto Carlos; Takebayashi Sassaki, Kikue
  • Caries Research, Vol. 39, Issue 6
  • DOI: 10.1159/000088189

Performance of a double-multilayer monochromator at Beamline 2-BM at the Advanced Photon Source
journal, March 2002

  • Chu, Y. S.; Liu, C.; Mancini, D. C.
  • Review of Scientific Instruments, Vol. 73, Issue 3
  • DOI: 10.1063/1.1423628

Effect of rinsing with water immediately after neutral gel and foam fluoride topical application on enamel remineralization: An in situ study
journal, November 2010


Effect of Fluoride, Lesion Baseline Severity and Mineral Distribution on Lesion Progression
journal, January 2012

  • Lippert, F.; Butler, A.; Lynch, R. J. M.
  • Caries Research, Vol. 46, Issue 1
  • DOI: 10.1159/000334787

Evaluation of Laser Fluorescence in the Monitoring of the Initial Stage of the De-/Remineralization Process: An in vitro and in situ Study
journal, January 2009

  • Spiguel, M. H.; Tovo, M. F.; Kramer, P. F.
  • Caries Research, Vol. 43, Issue 4
  • DOI: 10.1159/000218094

Calcium phosphate-based remineralization systems: scientific evidence?
journal, September 2008


Quantitative analysis of mineral content in enamel using synchrotron microtomography and microhardness analysis
conference, August 2006

  • Delbem, A. C. B.; Vieira, A. E. M.; Sassaki, K. T.
  • SPIE Optics + Photonics, SPIE Proceedings
  • DOI: 10.1117/12.681362

Effect of fluoride gels supplemented with sodium trimetaphosphate in reducing demineralization
journal, September 2013

  • Danelon, Marcelle; Takeshita, Eliana Mitsue; Peixoto, Ligia Carla
  • Clinical Oral Investigations, Vol. 18, Issue 4
  • DOI: 10.1007/s00784-013-1102-4

pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization
journal, January 2008


Amorphous calcium phosphate and its application in dentistry
journal, July 2011


Acid Susceptibility at Various Depths of pH-Cycled Enamel and Dentine Specimens
journal, December 2005

  • Lagerweij, M. D.; ten Cate, J. M.
  • Caries Research, Vol. 40, Issue 1
  • DOI: 10.1159/000088903

Enamel Subsurface Lesion Remineralisation with Casein Phosphopeptide Stabilised Solutions of Calcium, Phosphate and Fluoride
journal, January 2008

  • Cochrane, N. J.; Saranathan, S.; Cai, F.
  • Caries Research, Vol. 42, Issue 2
  • DOI: 10.1159/000113161

Works referencing / citing this record:

The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion
journal, January 2019

  • Thierens, Laurent A. M.; Moerman, Sophie; Elst, Charlotte van
  • Journal of Applied Oral Science, Vol. 27
  • DOI: 10.1590/1678-7757-2018-0589