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Title: Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers

Abstract

Here, we report an isomer specific IR-IR double resonance study of the mass-selected protonated triglycine peptide. Comparison of experimental spectra with calculations reveals the presence of two isomers, with protonation occurring at either the terminal amine site or one of the amide oxygen sites. The amine protonated isomer identified in our experiment contains an atypical cis amide configuration as well as a more typical trans amide. The amide protonated peptide, on the other hand, contains two trans amide moieties. Both isomers are found to be the lowest energy structures for their respective protonation site, but it is unclear, from experiments and calculations, which one is the global minimum. The presence of both in our experiments likely points to kinetic trapping of a higher energy structure. Lastly, the observed frequencies of the NH and OH stretch vibrations are used to estimate the hydrogen-bond strengths present in each isomer, accounting for the relative stabilities of these structures.

Authors:
 [1];  [1]; ORCiD logo [1]
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  1. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1434665
Alternate Identifier(s):
OSTI ID: 1691641
Grant/Contract Number:  
SC0010326
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Molecular Spectroscopy
Additional Journal Information:
Journal Volume: 347; Journal Issue: C; Journal ID: ISSN 0022-2852
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Infrared spectroscopy; Ions; Mass spectrometry; Peptide

Citation Formats

Voss, Jonathan M., Fischer, Kaitlyn C., and Garand, Etienne. Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers. United States: N. p., 2018. Web. doi:10.1016/j.jms.2018.03.006.
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Voss, Jonathan M., Fischer, Kaitlyn C., & Garand, Etienne. Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers. United States. https://doi.org/10.1016/j.jms.2018.03.006
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Voss, Jonathan M., Fischer, Kaitlyn C., and Garand, Etienne. Thu . "Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers". United States. https://doi.org/10.1016/j.jms.2018.03.006. https://www.osti.gov/servlets/purl/1434665.
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@article{osti_1434665,
title = {Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers},
author = {Voss, Jonathan M. and Fischer, Kaitlyn C. and Garand, Etienne},
abstractNote = {Here, we report an isomer specific IR-IR double resonance study of the mass-selected protonated triglycine peptide. Comparison of experimental spectra with calculations reveals the presence of two isomers, with protonation occurring at either the terminal amine site or one of the amide oxygen sites. The amine protonated isomer identified in our experiment contains an atypical cis amide configuration as well as a more typical trans amide. The amide protonated peptide, on the other hand, contains two trans amide moieties. Both isomers are found to be the lowest energy structures for their respective protonation site, but it is unclear, from experiments and calculations, which one is the global minimum. The presence of both in our experiments likely points to kinetic trapping of a higher energy structure. Lastly, the observed frequencies of the NH and OH stretch vibrations are used to estimate the hydrogen-bond strengths present in each isomer, accounting for the relative stabilities of these structures.},
doi = {10.1016/j.jms.2018.03.006},
journal = {Journal of Molecular Spectroscopy},
number = C,
volume = 347,
place = {United States},
year = {2018},
month = {3}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.jms.2018.03.006
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Cited by: 5 works
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Figures / Tables:

Figure 1 Figure 1: An example of a neutral triglycine molecule showing the numbering scheme of N and O groups starting from the amine terminal.
All figures and tables (8 total)
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Works referenced in this record:

Thermodynamics and Reaction Mechanisms of Decomposition of the Simplest Protonated Tripeptide, Triglycine: A Guided Ion Beam and Computational Study
journal, February 2017

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journal, January 2017

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Nmr studies of the molecular conformations in the linear oligopeptides H-( L -Ala) n - L -Pro-OH: MOLECULAR CONFORMATIONS
journal, October 1976

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    Works referencing / citing this record:

    The structures and properties of anionic tryptophan complexes
    journal, January 2018

    • Ieritano, Christian; Featherstone, Joshua; Carr, Patrick J. J.
    • Physical Chemistry Chemical Physics, Vol. 20, Issue 41
    • DOI: 10.1039/c8cp04533j
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      Figures / Tables found in this record:

      Figure 1 (p. 3)figure
      Figure 2 (p. 6)figure
      Table 1 (p. 7)table
      Figure 3 (p. 8)figure
      Figure 4 (p. 9)figure
      Table 2 (p. 10)table
      Figure 5 (p. 11)figure
      Table 3 (p. 15)table
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