Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers
Abstract
Here, we report an isomer specific IR-IR double resonance study of the mass-selected protonated triglycine peptide. Comparison of experimental spectra with calculations reveals the presence of two isomers, with protonation occurring at either the terminal amine site or one of the amide oxygen sites. The amine protonated isomer identified in our experiment contains an atypical cis amide configuration as well as a more typical trans amide. The amide protonated peptide, on the other hand, contains two trans amide moieties. Both isomers are found to be the lowest energy structures for their respective protonation site, but it is unclear, from experiments and calculations, which one is the global minimum. The presence of both in our experiments likely points to kinetic trapping of a higher energy structure. Lastly, the observed frequencies of the NH and OH stretch vibrations are used to estimate the hydrogen-bond strengths present in each isomer, accounting for the relative stabilities of these structures.
- Authors:
-
- Univ. of Wisconsin, Madison, WI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1434665
- Alternate Identifier(s):
- OSTI ID: 1691641
- Grant/Contract Number:
- SC0010326
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Molecular Spectroscopy
- Additional Journal Information:
- Journal Volume: 347; Journal Issue: C; Journal ID: ISSN 0022-2852
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Infrared spectroscopy; Ions; Mass spectrometry; Peptide
Citation Formats
Voss, Jonathan M., Fischer, Kaitlyn C., and Garand, Etienne. Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers. United States: N. p., 2018.
Web. doi:10.1016/j.jms.2018.03.006.
Voss, Jonathan M., Fischer, Kaitlyn C., & Garand, Etienne. Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers. United States. https://doi.org/10.1016/j.jms.2018.03.006
Voss, Jonathan M., Fischer, Kaitlyn C., and Garand, Etienne. Thu .
"Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers". United States. https://doi.org/10.1016/j.jms.2018.03.006. https://www.osti.gov/servlets/purl/1434665.
@article{osti_1434665,
title = {Revealing the structure of isolated peptides: IR-IR predissociation spectroscopy of protonated triglycine isomers},
author = {Voss, Jonathan M. and Fischer, Kaitlyn C. and Garand, Etienne},
abstractNote = {Here, we report an isomer specific IR-IR double resonance study of the mass-selected protonated triglycine peptide. Comparison of experimental spectra with calculations reveals the presence of two isomers, with protonation occurring at either the terminal amine site or one of the amide oxygen sites. The amine protonated isomer identified in our experiment contains an atypical cis amide configuration as well as a more typical trans amide. The amide protonated peptide, on the other hand, contains two trans amide moieties. Both isomers are found to be the lowest energy structures for their respective protonation site, but it is unclear, from experiments and calculations, which one is the global minimum. The presence of both in our experiments likely points to kinetic trapping of a higher energy structure. Lastly, the observed frequencies of the NH and OH stretch vibrations are used to estimate the hydrogen-bond strengths present in each isomer, accounting for the relative stabilities of these structures.},
doi = {10.1016/j.jms.2018.03.006},
journal = {Journal of Molecular Spectroscopy},
number = C,
volume = 347,
place = {United States},
year = {2018},
month = {3}
}
Web of Science
Figures / Tables:

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Works referencing / citing this record:
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