Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion
Abstract
Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl-tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids (ncAAs) into proteins. Introduction of such amino acids increases the chemical diversity of recombinant proteins endowing them with novel properties. Such proteins serve in sophisticated biochemical and biophysical studies both in vitro and in vivo, they may become unique biomaterials or therapeutic agents, and they afford metabolic dependence of genetically modified organisms for biocontainment purposes. In the Methanosarcinaceae the incorporation of the 22nd genetically encoded amino acid, pyrrolysine (Pyl), is facilitated by pyrrolysyl-tRNA synthetase (PylRS) and the cognate UAG-recognizing tRNAPyl. This unique aaRS•tRNA pair functions as an orthogonal translation system (OTS) in most model organisms. The facile directed evolution of the large PylRS active site to accommodate many ncAAs, and the enzyme’s anticodon-blind specific recognition of the cognate tRNAPyl make this system highly amenable for GCE purposes. The remarkable polyspecificity of PylRS has been exploited to incorporate >100 different ncAAs into proteins. Here we review the Pyl-OT system and selected GCE applications to examine the properties of an effective OTS.
- Authors:
-
- Yale Univ., New Haven, CT (United States)
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1434637
- Grant/Contract Number:
- FG02-98ER20311
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Croatica Chemica Acta
- Additional Journal Information:
- Journal Volume: 89; Journal Issue: 2; Journal ID: ISSN 0011-1643
- Publisher:
- The Croatian Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Crnkovic, Ana, Suzuki, Tateki, Soll, Dieter, and Reynolds, Noah M. Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion. United States: N. p., 2016.
Web. doi:10.5562/cca2825.
Crnkovic, Ana, Suzuki, Tateki, Soll, Dieter, & Reynolds, Noah M. Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion. United States. https://doi.org/10.5562/cca2825
Crnkovic, Ana, Suzuki, Tateki, Soll, Dieter, and Reynolds, Noah M. Tue .
"Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion". United States. https://doi.org/10.5562/cca2825. https://www.osti.gov/servlets/purl/1434637.
@article{osti_1434637,
title = {Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion},
author = {Crnkovic, Ana and Suzuki, Tateki and Soll, Dieter and Reynolds, Noah M.},
abstractNote = {Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl-tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids (ncAAs) into proteins. Introduction of such amino acids increases the chemical diversity of recombinant proteins endowing them with novel properties. Such proteins serve in sophisticated biochemical and biophysical studies both in vitro and in vivo, they may become unique biomaterials or therapeutic agents, and they afford metabolic dependence of genetically modified organisms for biocontainment purposes. In the Methanosarcinaceae the incorporation of the 22nd genetically encoded amino acid, pyrrolysine (Pyl), is facilitated by pyrrolysyl-tRNA synthetase (PylRS) and the cognate UAG-recognizing tRNAPyl. This unique aaRS•tRNA pair functions as an orthogonal translation system (OTS) in most model organisms. The facile directed evolution of the large PylRS active site to accommodate many ncAAs, and the enzyme’s anticodon-blind specific recognition of the cognate tRNAPyl make this system highly amenable for GCE purposes. The remarkable polyspecificity of PylRS has been exploited to incorporate >100 different ncAAs into proteins. Here we review the Pyl-OT system and selected GCE applications to examine the properties of an effective OTS.},
doi = {10.5562/cca2825},
journal = {Croatica Chemica Acta},
number = 2,
volume = 89,
place = {United States},
year = {Tue Jun 14 00:00:00 EDT 2016},
month = {Tue Jun 14 00:00:00 EDT 2016}
}
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Works referencing / citing this record:
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase
journal, October 2017
- Suzuki, Tateki; Miller, Corwin; Guo, Li-Tao
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tRNA Pyl : Structure, function, and applications
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Pyrrolysyl-tRNA Synthetase with a Unique Architecture Enhances the Availability of Lysine Derivatives in Synthetic Genetic Codes
journal, September 2018
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collection, January 2018
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- Apollo - University of Cambridge Repository
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase
journal, October 2017
- Suzuki, Tateki; Miller, Corwin; Guo, Li-Tao
- Nature Chemical Biology, Vol. 13, Issue 12
Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications
collection, January 2018
- Whitford, Christopher M.; Dymek, Saskia; Kerkhoff, Denise
- Apollo - University of Cambridge Repository
Recent Development of Genetic Code Expansion for Posttranslational Modification Studies
journal, July 2018
- Chen, Hao; Venkat, Sumana; McGuire, Paige
- Molecules, Vol. 23, Issue 7
Pyrrolysyl-tRNA Synthetase with a Unique Architecture Enhances the Availability of Lysine Derivatives in Synthetic Genetic Codes
journal, September 2018
- Yamaguchi, Atsushi; Iraha, Fumie; Ohtake, Kazumasa
- Molecules, Vol. 23, Issue 10