The Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner
Abstract
In plants, the presence of a load-bearing cell wall presents unique challenges during cell division. Unlike other eukaryotes, which undergo contractile cytokinesis upon completion of mitosis, plants instead synthesize and assemble a new dividing cell wall to separate newly formed daughter cells. In this study, we mine transcriptome data from individual cell types in the Arabidopsis thaliana stomatal lineage and identify CSLD5, a member of the Cellulose Synthase Like-D family, as a cell wall biosynthesis enzyme uniquely enriched in rapidly dividing cell populations. We further show that CSLD5 is a direct target of SPEECHLESS, the master transcriptional regulator of these divisions during stomatal development. Using a combination of genetic analysis and in vivo localization of fluorescently tagged fusion proteins, we show that CSLD5 preferentially accumulates in dividing plant cells where it participates in the construction of newly forming cell plates. We show that CSLD5 is an unstable protein that is rapidly degraded upon completion of cell division and that the protein turnover characteristics of CSLD5 are altered in ccs52a2 mutants, indicating that CSLD5 turnover may be regulated by a cell cycle-associated E3-ubiquitin ligase, the anaphase-promoting complex.
- Authors:
-
- Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Molecular, Cellular, and Developmental Biology
- Stanford Univ., CA (United States). Dept. of Biology
- Stanford Univ., CA (United States). Dept. of Biology and Howard Hughes Medical Inst.
- Publication Date:
- Research Org.:
- Univ. of Michigan, Ann Arbor, MI (United States)
- Sponsoring Org.:
- USDOE; National Science Foundation (NSF); National Institutes of Health (NIH); German Research Foundation (DFG); Howard Hughes Medical Inst., Chevy Chase, MD (United States)
- OSTI Identifier:
- 1434581
- Grant/Contract Number:
- FG02-07ER15887; 0937323; RO1GM086632
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Cell
- Additional Journal Information:
- Journal Volume: 28; Journal Issue: 7; Journal ID: ISSN 1040-4651
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Gu, Fangwei, Bringmann, Martin, Combs, Jonathon, Yang, Jiyuan, Bergmann, Dominique, and Nielsen, Erik. The Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner. United States: N. p., 2016.
Web. doi:10.1105/tpc.16.00203.
Gu, Fangwei, Bringmann, Martin, Combs, Jonathon, Yang, Jiyuan, Bergmann, Dominique, & Nielsen, Erik. The Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner. United States. https://doi.org/10.1105/tpc.16.00203
Gu, Fangwei, Bringmann, Martin, Combs, Jonathon, Yang, Jiyuan, Bergmann, Dominique, and Nielsen, Erik. Mon .
"The Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner". United States. https://doi.org/10.1105/tpc.16.00203. https://www.osti.gov/servlets/purl/1434581.
@article{osti_1434581,
title = {The Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner},
author = {Gu, Fangwei and Bringmann, Martin and Combs, Jonathon and Yang, Jiyuan and Bergmann, Dominique and Nielsen, Erik},
abstractNote = {In plants, the presence of a load-bearing cell wall presents unique challenges during cell division. Unlike other eukaryotes, which undergo contractile cytokinesis upon completion of mitosis, plants instead synthesize and assemble a new dividing cell wall to separate newly formed daughter cells. In this study, we mine transcriptome data from individual cell types in the Arabidopsis thaliana stomatal lineage and identify CSLD5, a member of the Cellulose Synthase Like-D family, as a cell wall biosynthesis enzyme uniquely enriched in rapidly dividing cell populations. We further show that CSLD5 is a direct target of SPEECHLESS, the master transcriptional regulator of these divisions during stomatal development. Using a combination of genetic analysis and in vivo localization of fluorescently tagged fusion proteins, we show that CSLD5 preferentially accumulates in dividing plant cells where it participates in the construction of newly forming cell plates. We show that CSLD5 is an unstable protein that is rapidly degraded upon completion of cell division and that the protein turnover characteristics of CSLD5 are altered in ccs52a2 mutants, indicating that CSLD5 turnover may be regulated by a cell cycle-associated E3-ubiquitin ligase, the anaphase-promoting complex.},
doi = {10.1105/tpc.16.00203},
journal = {The Plant Cell},
number = 7,
volume = 28,
place = {United States},
year = {Mon Jun 27 00:00:00 EDT 2016},
month = {Mon Jun 27 00:00:00 EDT 2016}
}
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