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Title: Design of a synthetic yeast genome

Authors:
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Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1434386
Grant/Contract Number:  
287313; FG02097ER25308
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Name: Science Journal Volume: 355 Journal Issue: 6329; Journal ID: ISSN 0036-8075
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
United States
Language:
English

Citation Formats

Richardson, Sarah M., Mitchell, Leslie A., Stracquadanio, Giovanni, Yang, Kun, Dymond, Jessica S., DiCarlo, James E., Lee, Dongwon, Huang, Cheng Lai Victor, Chandrasegaran, Srinivasan, Cai, Yizhi, Boeke, Jef D., and Bader, Joel S. Design of a synthetic yeast genome. United States: N. p., 2017. Web. doi:10.1126/science.aaf4557.
Richardson, Sarah M., Mitchell, Leslie A., Stracquadanio, Giovanni, Yang, Kun, Dymond, Jessica S., DiCarlo, James E., Lee, Dongwon, Huang, Cheng Lai Victor, Chandrasegaran, Srinivasan, Cai, Yizhi, Boeke, Jef D., & Bader, Joel S. Design of a synthetic yeast genome. United States. doi:10.1126/science.aaf4557.
Richardson, Sarah M., Mitchell, Leslie A., Stracquadanio, Giovanni, Yang, Kun, Dymond, Jessica S., DiCarlo, James E., Lee, Dongwon, Huang, Cheng Lai Victor, Chandrasegaran, Srinivasan, Cai, Yizhi, Boeke, Jef D., and Bader, Joel S. Thu . "Design of a synthetic yeast genome". United States. doi:10.1126/science.aaf4557.
@article{osti_1434386,
title = {Design of a synthetic yeast genome},
author = {Richardson, Sarah M. and Mitchell, Leslie A. and Stracquadanio, Giovanni and Yang, Kun and Dymond, Jessica S. and DiCarlo, James E. and Lee, Dongwon and Huang, Cheng Lai Victor and Chandrasegaran, Srinivasan and Cai, Yizhi and Boeke, Jef D. and Bader, Joel S.},
abstractNote = {},
doi = {10.1126/science.aaf4557},
journal = {Science},
number = 6329,
volume = 355,
place = {United States},
year = {2017},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aaf4557

Citation Metrics:
Cited by: 98 works
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Works referenced in this record:

The role of the loxP spacer region in PI site-specific recombination
journal, January 1986

  • Hoess, Ronald H.; Wierzbicki, Anna; Abremski, Kenneth
  • Nucleic Acids Research, Vol. 14, Issue 5
  • DOI: 10.1093/nar/14.5.2287

Bug mapping and fitness testing of chemically synthesized chromosome X
journal, March 2017


“Perfect” designer chromosome V and behavior of a ring derivative
journal, March 2017


Genetic manipulation of centromere function.
journal, July 1987


Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands
journal, January 1991

  • Ciccarelli, Richard B.; Gunyuzlu, Paul; Huang, James
  • Nucleic Acids Research, Vol. 19, Issue 21
  • DOI: 10.1093/nar/19.21.6007

Chromosome-Scale Genetic Mapping Using a Set of 16 Conditionally Stable Saccharomyces cerevisiae Chromosomes
journal, October 2008


Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational folding
journal, December 2012

  • Pechmann, Sebastian; Frydman, Judith
  • Nature Structural & Molecular Biology, Vol. 20, Issue 2
  • DOI: 10.1038/nsmb.2466

Life with 6000 Genes
journal, October 1996


The Saccharomyces cerevisiae SCRaMbLE system and genome minimization
journal, May 2012


JBrowse: A next-generation genome browser
journal, July 2009

  • Skinner, M. E.; Uzilov, A. V.; Stein, L. D.
  • Genome Research, Vol. 19, Issue 9
  • DOI: 10.1101/gr.094607.109

Intragenic tandem repeats generate functional variability
journal, August 2005

  • Verstrepen, Kevin J.; Jansen, An; Lewitter, Fran
  • Nature Genetics, Vol. 37, Issue 9
  • DOI: 10.1038/ng1618

Introns within Ribosomal Protein Genes Regulate the Production and Function of Yeast Ribosomes
journal, October 2011


Rapid generation of DNA fragments by PCR amplification of crude, synthetic oligonucleotides
journal, January 1990


GeneDesign 3.0 is an updated synthetic biology toolkit
journal, March 2010

  • Richardson, Sarah M.; Nunley, Paul W.; Yarrington, Robert M.
  • Nucleic Acids Research, Vol. 38, Issue 8
  • DOI: 10.1093/nar/gkq143

Saccharomyces cerevisiae S288C genome annotation: a working hypothesis
journal, January 2006

  • Fisk, Dianna G.; Ball, Catherine A.; Dolinski, Kara
  • Yeast, Vol. 23, Issue 12
  • DOI: 10.1002/yea.1400

An Evolutionarily Conserved Mechanism for Controlling the Efficiency of Protein Translation
journal, April 2010


Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond
journal, March 2017

  • Mitchell, Leslie A.; Wang, Ann; Stracquadanio, Giovanni
  • Science, Vol. 355, Issue 6329
  • DOI: 10.1126/science.aaf4831

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement
journal, July 2011

  • Isaacs, Farren J.; Carr, Peter A.; Wang, Harris H.
  • Science, Vol. 333, Issue 6040, p. 348-353
  • DOI: 10.1126/science.1205822

SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes
journal, November 2015

  • Shen, Yue; Stracquadanio, Giovanni; Wang, Yun
  • Genome Research, Vol. 26, Issue 1
  • DOI: 10.1101/gr.193433.115

Patterns of Codon Usage in two Ciliates that Reassign the Genetic Code: Tetrahymena thermophila and Paramecium tetraurelia
journal, April 2008


BLAST+: architecture and applications
journal, January 2009

  • Camacho, Christiam; Coulouris, George; Avagyan, Vahram
  • BMC Bioinformatics, Vol. 10, Issue 1
  • DOI: 10.1186/1471-2105-10-421

Probing the Limits of Genetic Recoding in Essential Genes
journal, October 2013


Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes
journal, January 1986

  • Sharp, Paul M.; Tuohy, Therese M. F.; Mosurski, Krzysztof R.
  • Nucleic Acids Research, Vol. 14, Issue 13
  • DOI: 10.1093/nar/14.13.5125

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design
journal, September 2011

  • Dymond, Jessica S.; Richardson, Sarah M.; Coombes, Candice E.
  • Nature, Vol. 477, Issue 7365
  • DOI: 10.1038/nature10403

Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project
journal, August 2015


Circular permutation of a synthetic eukaryotic chromosome with the telomerator
journal, November 2014

  • Mitchell, Leslie A.; Boeke, Jef D.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 48
  • DOI: 10.1073/pnas.1414399111

Engineering the ribosomal DNA in a megabase synthetic chromosome
journal, March 2017


Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome
journal, March 2017


Total Synthesis of a Functional Designer Eukaryotic Chromosome
journal, March 2014

  • Annaluru, Narayana; Muller, Héloïse; Mitchell, Leslie A.
  • Science, Vol. 344, Issue 6179
  • DOI: 10.1126/science.1249252

SGD: Saccharomyces Genome Database
journal, January 1998


GeneDesign: Rapid, automated design of multikilobase synthetic genes
journal, March 2006


Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009

  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318

Deletion of Many Yeast Introns Reveals a Minority of Genes that Require Splicing for Function
journal, May 2008

  • Parenteau, Julie; Durand, Mathieu; Véronneau, Steeve
  • Molecular Biology of the Cell, Vol. 19, Issue 5
  • DOI: 10.1091/mbc.E07-12-1254

The Generic Genome Browser: A Building Block for a Model Organism System Database
journal, October 2002