skip to main content

DOE PAGESDOE PAGES

Title: Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallography with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. Here, the method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.
Authors:
 [1] ; ORCiD logo [1] ;  [1] ;  [1] ; ORCiD logo [1] ;  [2] ;  [3] ;  [1] ; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Columbia Univ., New York, NY (United States)
Publication Date:
Report Number(s):
BNL-203558-2018-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ
Grant/Contract Number:
SC0012704; KC0401040; AC02-06CH11357
Type:
Published Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microcrystals; microdiffraction; radiation damage; data analysis; multiple crystals; X-ray crystallography; structural biology; microcrystal
OSTI Identifier:
1433744
Alternate Identifier(s):
OSTI ID: 1434774; OSTI ID: 1466304