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Title: Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles

To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5-10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica. Time-lapse images of gold-capped MSN stationed in microchannels reveal that the rate of gold release is dependent on the concentration of the cleaving agent. Finally, the uncapping process was also monitored successfully for MSN endocytosed by A549 cancer cells, which produce the cleaving agent glutathione. These experiments demonstrate that the optical properties of MSN can be used to directly monitor cleaving kinetics, even in complex cellular settings.
Authors:
 [1] ; ORCiD logo [1] ; ORCiD logo [2] ;  [3] ; ORCiD logo [4]
  1. Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States)
  2. Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Colorado School of Mines, Golden, CO (United States). Dept. of Chemistry
  3. Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Georgia State Univ., Atlanta, GA (United States). Dept. of Chemistry
  4. Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Ohio Univ., Athens, OH (United States). Dept. of Chemistry and Biochemistry
Publication Date:
Report Number(s):
IS-J-9626
Journal ID: ISSN 0003-2700
Grant/Contract Number:
AC02-07CH11358
Type:
Accepted Manuscript
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 90; Journal Issue: 5; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1433666

Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, and Stender, Anthony S.. Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles. United States: N. p., Web. doi:10.1021/acs.analchem.7b04532.
Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, & Stender, Anthony S.. Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles. United States. doi:10.1021/acs.analchem.7b04532.
Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, and Stender, Anthony S.. 2018. "Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles". United States. doi:10.1021/acs.analchem.7b04532.
@article{osti_1433666,
title = {Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles},
author = {Augspurger, Ashley E. and Sun, Xiaoxing and Trewyn, Brian G. and Fang, Ning and Stender, Anthony S.},
abstractNote = {To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5-10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica. Time-lapse images of gold-capped MSN stationed in microchannels reveal that the rate of gold release is dependent on the concentration of the cleaving agent. Finally, the uncapping process was also monitored successfully for MSN endocytosed by A549 cancer cells, which produce the cleaving agent glutathione. These experiments demonstrate that the optical properties of MSN can be used to directly monitor cleaving kinetics, even in complex cellular settings.},
doi = {10.1021/acs.analchem.7b04532},
journal = {Analytical Chemistry},
number = 5,
volume = 90,
place = {United States},
year = {2018},
month = {2}
}