Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles

Augspurger, Ashley E.; Sun, Xiaoxing; Trewyn, Brian G.; Fang, Ning; Stender, Anthony S.

doi:10.1021/acs.analchem.7b04532

Title: Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles

Abstract

To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5-10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica. Time-lapse images of gold-capped MSN stationed in microchannels reveal that the rate of gold release is dependent on the concentration of the cleaving agent. Finally, the uncapping process was also monitored successfully for MSN endocytosed by A549 cancer cells, which produce the cleaving agent glutathione. These experiments demonstrate that the optical properties of MSN can be used to directly monitor cleaving kinetics, even in complex cellular settings.

Authors:

Augspurger, Ashley E. ^[1];

^[1];

^[2]; Fang, Ning ^[3];

^[4]

Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States)
Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Colorado School of Mines, Golden, CO (United States). Dept. of Chemistry
Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Georgia State Univ., Atlanta, GA (United States). Dept. of Chemistry
Iowa State Univ., Ames, IA (United States). Dept. of Chemistry; Ames Lab., Ames, IA (United States); Ohio Univ., Athens, OH (United States). Dept. of Chemistry and Biochemistry

Publication Date:: 2018-02-05

Research Org.:: Ames Lab., Ames, IA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1433666

Report Number(s):: IS-J-9626
Journal ID: ISSN 0003-2700; TRN: US1802665

Grant/Contract Number:: AC02-07CH11358

Resource Type:: Accepted Manuscript

Journal Name:: Analytical Chemistry

Additional Journal Information:: Journal Volume: 90; Journal Issue: 5; Journal ID: ISSN 0003-2700

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 77 NANOSCIENCE AND NANOTECHNOLOGY; 36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, and Stender, Anthony S. Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles.  United States: N. p., 2018. 
Web.  doi:10.1021/acs.analchem.7b04532.

Copy to clipboard


                    Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, & Stender, Anthony S. Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles.  United States.  https://doi.org/10.1021/acs.analchem.7b04532

Copy to clipboard


                    Augspurger, Ashley E., Sun, Xiaoxing, Trewyn, Brian G., Fang, Ning, and Stender, Anthony S. Mon .  
"Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles".  United States.  https://doi.org/10.1021/acs.analchem.7b04532.  https://www.osti.gov/servlets/purl/1433666.

Copy to clipboard


                    
@article{osti_1433666,

  title        = {Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles},

  author       = {Augspurger, Ashley E. and Sun, Xiaoxing and Trewyn, Brian G. and Fang, Ning and Stender, Anthony S.},

  abstractNote = {To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5-10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica. Time-lapse images of gold-capped MSN stationed in microchannels reveal that the rate of gold release is dependent on the concentration of the cleaving agent. Finally, the uncapping process was also monitored successfully for MSN endocytosed by A549 cancer cells, which produce the cleaving agent glutathione. These experiments demonstrate that the optical properties of MSN can be used to directly monitor cleaving kinetics, even in complex cellular settings.},

  doi          = {10.1021/acs.analchem.7b04532},

  journal      = {Analytical Chemistry},

  number       = 5,

  volume       = 90,

  place        = {United States},

  year         = {2018},

  month        = {2}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/acs.analchem.7b04532

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 1 work

Citation information provided by
Web of Science

Figures / Tables:

Figure 1: MSN used in this study. A) Cross-section of a bare MSN, revealing its interior channels. B) AuNP-capped MSN with AuNP capping pore openings. C) Gold-plated MSN with gold decorating the interior channels and the MSN exterior. TEM images of D) bare hexagonal MSN, E) gold-plated MSN, F) MSNmore »

All figures and tables (4 total)

Save / Share:

Export Metadata

Save to My Library

Figures / Tables found in this record:

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

Similar Records in DOE PAGES and OSTI.GOV collections:

Fine-tuning the release of molecular guests from mesoporous silicas by controlling the orientation and mobility of surface phenyl substituents

Journal Article Manzano, J. Sebastian ; Singappuli-Arachchige, Dilini ; Parikh, Bosky L. ; ... - Chemical Engineering Journal

Phenyl-functionalized mesoporous silica materials were used to explore the effect of non-covalent interactions on the release of Ibuprofen into simulated body fluid. Variations in orientation and conformational mobility of the surface phenyl groups were introduced by selecting different structural precursors: a rigid upright orientation was obtained using phenyl groups directly bound to surface Si atoms (Ph-MSN), mobile groups were produced by using ethylene linkers to connect phenyl groups to the surface (PhEt-MSN), and groups co-planar to the surface were obtained by synthesizing a phenylene-bridged periodic mesoporous organosilica (Ph-PMO). The Ibuprofen release profiles from these materials and non-functionalized mesoporous silica nanoparticlesmore »« less
Cited by 1
https://doi.org/10.1016/j.cej.2017.12.015

Full Text Available
Gold Functionalized Mesoporous Silica Nanoparticle Mediated Protein and DNA Codelivery to Plant Cells Via the Biolistic Method

Journal Article Martin-Ortigosa, Susana ; Valenstein, Justin S ; Lin, Victor S.-Y. ; ... - Advanced Functional Materials

The synthesis and characterization of a gold nanoparticle functionalized mesoporous silica nanoparticle (Au-MSN) platform for codelivery of proteins and plasmid DNA to plant tissues using a biolistic particle delivery system is reported. The in vitro uptake and release profiles of fluorescently labeled bovine serum albumin (BSA) and enhanced green fluorescent protein (eGFP) are investigated. As a proof-of-concept demonstration, Au-MSN with large average pore diameters (10 nm) are shown to deliver and subsequently release proteins and plasmid DNA to the same cell after passing through the plant cell wall upon bombardment. Release of fluorescent eGFP indicates the delivery of active, non-denaturedmore »« less
https://doi.org/10.1002/adfm.201200359
Mesoporous silica nanoparticles for biomedical and catalytical applications

Thesis/Dissertation Sun, Xiaoxing

Mesoporous silica materials, discovered in 1992 by the Mobile Oil Corporation, have received considerable attention in the chemical industry due to their superior textual properties such as high surface area, large pore volume, tunable pore diameter, and narrow pore size distribution. Among those materials, MCM-41, referred to Mobile Composition of Matter NO. 41, contains honeycomb liked porous structure that is the most common mesoporous molecular sieve studied. Applications of MCM-41 type mesoporous silica material in biomedical field as well as catalytical field have been developed and discussed in this thesis. The unique features of mesoporous silica nanoparticles were utilized formore »« less
https://doi.org/10.2172/1029607

Full Text Available
ndocytosis of a Single Mesoporous Silica Nanoparticle into a Human Lung Cancer Cell Observed by Differential Interference Contrast Microscopy

Journal Article Sun, Wei ; Fang, Ning ; Trewyn, Brian ; ... - Anal Bioanal Chem

The unique structural features of mesoporous silica nanoparticles (MSN) have made them very useful in biological applications, such as gene therapy and drug delivery. Flow cytometry, confocal microscopy, and electron microscopy have been used for observing the endocytosis of MSN. However, flow cytometry cannot directly observe the process of endocytosis. Confocal microscopy requires fluorescence labeling of the cells. Electron microscopy can only utilize fixed cells. In the present work, we demonstrate for the first time that differential interference contrast (DIC) microscopy can be used to observe the entire endocytosis process of MSN into living human lung cancer cells (A549) withoutmore »« less
https://doi.org/10.1007/s00216-008-2162-1
Mesoporous silica-encapsulated gold core–shell nanoparticles for active solvent-free benzyl alcohol oxidation

Journal Article Hammond-Pereira, Ellis ; Bryant, Kristin ; Graham, Trenton R. ; ... - Reaction Chemistry & Engineering

Silica-encapsulated gold core@shell nanoparticles (Au@SiO2 CSNPs) were synthesized via a bottom-up procedure and used to catalyze the selective oxidation of benzyl alcohol. The pore size, morphology, crystallinity and composition of Au@SiO2 was evaluated using non-local density functional theory, transmission electron microscopy, high-energy x-ray diffraction and inductively coupled plasma-mass spectroscopy, respectively. The nanoparticles exhibit a mesoporous shell with an average thickness of 25.5 Å which can enhance selectivity via preferential transport of the desired product (i.e., benzaldehyde) relative to larger, undesired products (i.e, benzoic acid/benzyl benzoate). GC-FID analysis revealed the addition of potassium carbonate to the solvent-free oxidation of benzyl alcoholmore »« less
https://doi.org/10.1039/D0RE00198H

Similar Records