Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
Abstract
Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
- Authors:
-
[1];
[3]
- Zhejiang Univ., Hangzhou (China). College of Chemical and Biological Engineering, Key Lab. of Biomass Chemical Engineering of Ministry of Education
- Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology, Dept. of Chemical and Biomolecular Engineering
- Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology, Dept. of Chemical and Biomolecular Engineering; Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Chemistry, Biochemistry, and Bioengineering
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Zhejiang Univ., Hangzhou (China)
- OSTI Identifier:
- 1436426
- Alternate Identifier(s):
- OSTI ID: 1433560; OSTI ID: 1435935
- Grant/Contract Number:
- SC0018420; SC0018260
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology Journal
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1860-6768
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Gene regulation; Genome engineering; Metabolic engineering; Saccharomyces cerevisiae; Synthetic biology; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; gene regulation; genome engineering; metabolic engineering; saccaromyces cerevisiae; synthetic biology
Citation Formats
Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States: N. p., 2018.
Web. doi:10.1002/biot.201700601.
Lian, Jiazhang, HamediRad, Mohammad, & Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States. https://doi.org/10.1002/biot.201700601
Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. Tue .
"Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System". United States. https://doi.org/10.1002/biot.201700601. https://www.osti.gov/servlets/purl/1436426.
@article{osti_1436426,
title = {Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System},
author = {Lian, Jiazhang and HamediRad, Mohammad and Zhao, Huimin},
abstractNote = {Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.},
doi = {10.1002/biot.201700601},
journal = {Biotechnology Journal},
number = 9,
volume = 13,
place = {United States},
year = {Tue Feb 13 00:00:00 EST 2018},
month = {Tue Feb 13 00:00:00 EST 2018}
}
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Figure 1: CRISPR for genome editing and transcriptional regulation. (A) CRISPR-mediated double-strand break and subsequent homology directed repair (HDR). (B) CRISPR-mediated transcriptional regulation by recruiting an effector domain (activator or repressor domain) to the nuclease-deficient CRISPR complex, via dCas9 fusion (upper panel) or scRNA design (specific aptamer-RNA binding protein interaction,more »
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