Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana

Wang, Kun; Guo, Qiang; Froehlich, John E.; Hersh, Hope Lynn; Zienkiewicz, Agnieszka; Howe, Gregg A.; Benning, Christoph

doi:10.1105/tpc.18.00250

Title: Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana

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Abstract

Chloroplast membranes with their unique lipid composition are crucial for photosynthesis. Maintenance of the chloroplast membranes requires finely tuned lipid anabolic and catabolic reactions. Despite the presence of a large number of predicted lipid-degrading enzymes in the chloroplasts, their biological functions remain largely unknown. Recently, we described PLASTID LIPASE1 (PLIP1), a plastid phospholipase A₁ that contributes to seed oil biosynthesis. The Arabidopsis thaliana genome encodes two putative PLIP1 paralogs, which we designated PLIP2 and PLIP3. PLIP2 and PLIP3 are also present in the chloroplasts, but likely with different subplastid locations. In vitro analysis indicated that both are glycerolipid A₁ lipases. In vivo, PLIP2 prefers monogalactosyldiacylglycerol as substrate and PLIP3 phosphatidylglycerol. Overexpression of PLIP2 or PLIP3 severely reduced plant growth and led to accumulation of the bioactive form of jasmonate and related oxylipins. Genetically blocking jasmonate perception restored the growth of the PLIP2/3-overexpressing plants. The expression of PLIP2 and PLIP3, but not PLIP1, was induced by abscisic acid (ABA), and plip1 plip2 plip3 triple mutants exhibited compromised oxylipin biosynthesis in response to ABA. The plip triple mutants also showed hypersensitivity to ABA. Here, we propose that PLIP2 and PLIP3 provide a mechanistic link between ABA-mediated abiotic stress responses and oxylipin signaling.

Authors:

^[1];

^[1]; Froehlich, John E. ^[1]; Hersh, Hope Lynn ^[1];

^[2];

^[3];

^[2]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Great Lakes Bioenergy Research Center
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory, Great Lakes Bioenergy Research Center, and Plant Resilience Inst.

Publication Date:: Tue Apr 17 00:00:00 EDT 2018

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1433426

Grant/Contract Number:: FG02-98ER20305; FG02-91ER20021; FC02-07ER64494

Resource Type:: Published Article

Journal Name:: The Plant Cell

Additional Journal Information:: Journal Name: The Plant Cell Journal Volume: 30 Journal Issue: 5; Journal ID: ISSN 1040-4651

Publisher:: American Society of Plant Biologists

Country of Publication:: United States

Language:: English

Citation Formats


                    Wang, Kun, Guo, Qiang, Froehlich, John E., Hersh, Hope Lynn, Zienkiewicz, Agnieszka, Howe, Gregg A., and Benning, Christoph. Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana.  United States: N. p., 2018. 
Web.  doi:10.1105/tpc.18.00250.

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                    Wang, Kun, Guo, Qiang, Froehlich, John E., Hersh, Hope Lynn, Zienkiewicz, Agnieszka, Howe, Gregg A., & Benning, Christoph. Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana.  United States.  https://doi.org/10.1105/tpc.18.00250

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                    Wang, Kun, Guo, Qiang, Froehlich, John E., Hersh, Hope Lynn, Zienkiewicz, Agnieszka, Howe, Gregg A., and Benning, Christoph. Tue .  
"Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana".  United States.  https://doi.org/10.1105/tpc.18.00250.

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@article{osti_1433426,

  title        = {Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana},

  author       = {Wang, Kun and Guo, Qiang and Froehlich, John E. and Hersh, Hope Lynn and Zienkiewicz, Agnieszka and Howe, Gregg A. and Benning, Christoph},

  abstractNote = {Chloroplast membranes with their unique lipid composition are crucial for photosynthesis. Maintenance of the chloroplast membranes requires finely tuned lipid anabolic and catabolic reactions. Despite the presence of a large number of predicted lipid-degrading enzymes in the chloroplasts, their biological functions remain largely unknown. Recently, we described PLASTID LIPASE1 (PLIP1), a plastid phospholipase A1 that contributes to seed oil biosynthesis. The Arabidopsis thaliana genome encodes two putative PLIP1 paralogs, which we designated PLIP2 and PLIP3. PLIP2 and PLIP3 are also present in the chloroplasts, but likely with different subplastid locations. In vitro analysis indicated that both are glycerolipid A1 lipases. In vivo, PLIP2 prefers monogalactosyldiacylglycerol as substrate and PLIP3 phosphatidylglycerol. Overexpression of PLIP2 or PLIP3 severely reduced plant growth and led to accumulation of the bioactive form of jasmonate and related oxylipins. Genetically blocking jasmonate perception restored the growth of the PLIP2/3-overexpressing plants. The expression of PLIP2 and PLIP3, but not PLIP1, was induced by abscisic acid (ABA), and plip1 plip2 plip3 triple mutants exhibited compromised oxylipin biosynthesis in response to ABA. The plip triple mutants also showed hypersensitivity to ABA. Here, we propose that PLIP2 and PLIP3 provide a mechanistic link between ABA-mediated abiotic stress responses and oxylipin signaling.},

  doi          = {10.1105/tpc.18.00250},

  journal      = {The Plant Cell},

  number       = 5,

  volume       = 30,

  place        = {United States},

  year         = {Tue Apr 17 00:00:00 EDT 2018},

  month        = {Tue Apr 17 00:00:00 EDT 2018}

}

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