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Title: An alternate mode of oligomerization for E. coli SecA

Abstract

SecA is the ATPase of preprotein translocase. SecA is a dimer in solution and changes in its oligomeric state may function in preprotein translocation. The SecA-N68 construct, in which the C-terminal helical domains of SecA are deleted, was used to investigate the mechanism of SecA oligomerization. SecA-N68 is in equilibrium between monomers, dimers, and tetramers. Subunit interactions in the SecA-N68 tetramer are mediated entirely by unstructured regions at its N- and C-termini: when the termini are deleted to yield SecA-N68ΔNC, the construct is completely monomeric. This monomeric construct yielded crystals diffracting to 2.6 Å that were used to solve the structure of SecA-N68, including the “preprotein crosslinking domain” (PPXD) that was missing from previous E. coli SecA structures. The SecA-N68 structure was combined with small angle X-ray scattering (SAXS) data to construct a model of the SecA-N68 tetramer that is consistent with the essential roles of the extreme N- and C-termini in oligomerization. This mode of oligomerization, which depends on binding of the extreme N-terminus to the DEAD motor domains, NBD1 and NBD2, was used to model a novel parallel and flexible SecA solution dimer that agrees well with SAXS data.

Authors:
 [1];  [1];  [1]
  1. Univ. of Western Ontario, London, ON (Canada)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health
OSTI Identifier:
1432853
Grant/Contract Number:  
[W-31-109-ENG-38; RR-08630]
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
[ Journal Volume: 7; Journal Issue: 1]; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Chaperones; Protein translocation; SAXS; X-ray crystallography

Citation Formats

Yazdi, Aliakbar Khalili, Vezina, Grant C., and Shilton, Brian H. An alternate mode of oligomerization for E. coli SecA. United States: N. p., 2017. Web. doi:10.1038/s41598-017-11648-5.
Yazdi, Aliakbar Khalili, Vezina, Grant C., & Shilton, Brian H. An alternate mode of oligomerization for E. coli SecA. United States. doi:10.1038/s41598-017-11648-5.
Yazdi, Aliakbar Khalili, Vezina, Grant C., and Shilton, Brian H. Mon . "An alternate mode of oligomerization for E. coli SecA". United States. doi:10.1038/s41598-017-11648-5. https://www.osti.gov/servlets/purl/1432853.
@article{osti_1432853,
title = {An alternate mode of oligomerization for E. coli SecA},
author = {Yazdi, Aliakbar Khalili and Vezina, Grant C. and Shilton, Brian H.},
abstractNote = {SecA is the ATPase of preprotein translocase. SecA is a dimer in solution and changes in its oligomeric state may function in preprotein translocation. The SecA-N68 construct, in which the C-terminal helical domains of SecA are deleted, was used to investigate the mechanism of SecA oligomerization. SecA-N68 is in equilibrium between monomers, dimers, and tetramers. Subunit interactions in the SecA-N68 tetramer are mediated entirely by unstructured regions at its N- and C-termini: when the termini are deleted to yield SecA-N68ΔNC, the construct is completely monomeric. This monomeric construct yielded crystals diffracting to 2.6 Å that were used to solve the structure of SecA-N68, including the “preprotein crosslinking domain” (PPXD) that was missing from previous E. coli SecA structures. The SecA-N68 structure was combined with small angle X-ray scattering (SAXS) data to construct a model of the SecA-N68 tetramer that is consistent with the essential roles of the extreme N- and C-termini in oligomerization. This mode of oligomerization, which depends on binding of the extreme N-terminus to the DEAD motor domains, NBD1 and NBD2, was used to model a novel parallel and flexible SecA solution dimer that agrees well with SAXS data.},
doi = {10.1038/s41598-017-11648-5},
journal = {Scientific Reports},
number = [1],
volume = [7],
place = {United States},
year = {2017},
month = {9}
}

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