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Title: Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM

Abstract

We previously described a plasmid of Agrobacterium spp., pAoF64/95, in which the quorum-sensing system that controls conjugative transfer is induced by the opine mannopine. We also showed that the quorum-sensing regulators TraR, TraM, and TraI function similarly to their counterparts in other repABC plasmids. However, traR, unlike its counterpart on Ti plasmids, is monocistronic and not located in an operon that is inducible by the conjugative opine. Here, we report that both traR and traM are expressed constitutively and not regulated by growth with mannopine. We report two additional regulatory genes, mrtR and tmsP, that are involved in a novel mechanism of control of TraR activity. Both genes are located in the distantly linked region of pAoF64/95 encoding mannopine utilization. MrtR, in the absence of mannopine, represses the four-gene mocC operon as well as tmsP, which is the distal gene of the eight-gene motA operon. As judged by a bacterial two-hybrid analysis, TmsP, which shows amino acid sequence relatedness with the TraM-binding domain of TraR, interacts with the antiactivator. We propose a model in which mannopine, acting through the repressor MrtR, induces expression of TmsP which then titrates the levels of TraM thereby freeing TraR to activate the tra regulon.

Authors:
ORCiD logo [1];  [2];  [2]; ORCiD logo [1]
  1. Department of Microbiology, The University of Illinois at Urbana-Champaign, Urbana IL USA
  2. School of Agriculture, Food and Wine, The University of Adelaide, Osmond SA Australia
Publication Date:
Research Org.:
Univ. of Notre Dame, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Institutes of Health (NIH)
OSTI Identifier:
1432593
Alternate Identifier(s):
OSTI ID: 1432594; OSTI ID: 1610878
Grant/Contract Number:  
SC0006642; R01 GM52465
Resource Type:
Published Article
Journal Name:
MicrobiologyOpen
Additional Journal Information:
Journal Name: MicrobiologyOpen Journal Volume: 8 Journal Issue: 1; Journal ID: ISSN 2045-8827
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microbiology; agrobacterium; bacterial plasmids; gene regulation; quorum-sensing

Citation Formats

Wetzel, Margaret E., Asenstorfer, Robert E., Tate, Max E., and Farrand, Stephen K. Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM. United Kingdom: N. p., 2018. Web. doi:10.1002/mbo3.625.
Wetzel, Margaret E., Asenstorfer, Robert E., Tate, Max E., & Farrand, Stephen K. Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM. United Kingdom. doi:10.1002/mbo3.625.
Wetzel, Margaret E., Asenstorfer, Robert E., Tate, Max E., and Farrand, Stephen K. Tue . "Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM". United Kingdom. doi:10.1002/mbo3.625.
@article{osti_1432593,
title = {Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM},
author = {Wetzel, Margaret E. and Asenstorfer, Robert E. and Tate, Max E. and Farrand, Stephen K.},
abstractNote = {We previously described a plasmid of Agrobacterium spp., pAoF64/95, in which the quorum-sensing system that controls conjugative transfer is induced by the opine mannopine. We also showed that the quorum-sensing regulators TraR, TraM, and TraI function similarly to their counterparts in other repABC plasmids. However, traR, unlike its counterpart on Ti plasmids, is monocistronic and not located in an operon that is inducible by the conjugative opine. Here, we report that both traR and traM are expressed constitutively and not regulated by growth with mannopine. We report two additional regulatory genes, mrtR and tmsP, that are involved in a novel mechanism of control of TraR activity. Both genes are located in the distantly linked region of pAoF64/95 encoding mannopine utilization. MrtR, in the absence of mannopine, represses the four-gene mocC operon as well as tmsP, which is the distal gene of the eight-gene motA operon. As judged by a bacterial two-hybrid analysis, TmsP, which shows amino acid sequence relatedness with the TraM-binding domain of TraR, interacts with the antiactivator. We propose a model in which mannopine, acting through the repressor MrtR, induces expression of TmsP which then titrates the levels of TraM thereby freeing TraR to activate the tra regulon.},
doi = {10.1002/mbo3.625},
journal = {MicrobiologyOpen},
number = 1,
volume = 8,
place = {United Kingdom},
year = {2018},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/mbo3.625

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