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Title: A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology

Abstract

ABSTRACT Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation tolerance and release of unicellular versus multicellular progeny. Live cell staining and time-lapse confocal imaging coupled with transmission electron microscopy established that the desert and aquatic species all divide by multiple (rather than binary) fission, although progeny were unicellular in three species and multicellular (joined in a sheet-like coenobium) in two. During division, Golgi complexes were localized near nuclei, and all species exhibited dynamic rotation of the daughter cell mass within the mother cell wall at cytokinesis. Differential desiccation tolerance across the five species, assessed from photosynthetic efficiency during desiccation/rehydration cycles, was accompanied by differential accumulation of intracellular reactive oxygen species (ROS) detected using a dye sensitive to intracellular ROS. Further comparative investigation will aim to understand the genetic, ultrastructural and physiological characteristics supporting unicellular versus multicellular coenobial morphology, and the ability of representatives in the Scenedesmaceae to colonize ecologically diverse, even extreme, habitats.

Authors:
ORCiD logo [1];  [1];  [2];  [3];  [4]
  1. Ecosystems Center, Marine Biological Laboratory, Woods Hole, MA 02543 USA
  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352 USA
  3. Biological Sciences and Environmental Studies Departments, Mt. Holyoke College, South Hadley, MA 01075 USA
  4. Department of Biology, University of Massachusetts, Amherst, MA 01003 USA, Whitman Center, Marine Biological Laboratory, Woods Hole, MA 02543 USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1432568
Grant/Contract Number:  
48938
Resource Type:
Published Article
Journal Name:
Journal of Cell Science
Additional Journal Information:
Journal Name: Journal of Cell Science Journal Volume: 131 Journal Issue: 7; Journal ID: ISSN 0021-9533
Publisher:
The Company of Biologists
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Cardon, Zoe G., Peredo, Elena L., Dohnalkova, Alice C., Gershone, Hannah L., and Bezanilla, Magdalena. A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology. United Kingdom: N. p., 2018. Web. https://doi.org/10.1242/jcs.212233.
Cardon, Zoe G., Peredo, Elena L., Dohnalkova, Alice C., Gershone, Hannah L., & Bezanilla, Magdalena. A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology. United Kingdom. https://doi.org/10.1242/jcs.212233
Cardon, Zoe G., Peredo, Elena L., Dohnalkova, Alice C., Gershone, Hannah L., and Bezanilla, Magdalena. Tue . "A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology". United Kingdom. https://doi.org/10.1242/jcs.212233.
@article{osti_1432568,
title = {A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology},
author = {Cardon, Zoe G. and Peredo, Elena L. and Dohnalkova, Alice C. and Gershone, Hannah L. and Bezanilla, Magdalena},
abstractNote = {ABSTRACT Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation tolerance and release of unicellular versus multicellular progeny. Live cell staining and time-lapse confocal imaging coupled with transmission electron microscopy established that the desert and aquatic species all divide by multiple (rather than binary) fission, although progeny were unicellular in three species and multicellular (joined in a sheet-like coenobium) in two. During division, Golgi complexes were localized near nuclei, and all species exhibited dynamic rotation of the daughter cell mass within the mother cell wall at cytokinesis. Differential desiccation tolerance across the five species, assessed from photosynthetic efficiency during desiccation/rehydration cycles, was accompanied by differential accumulation of intracellular reactive oxygen species (ROS) detected using a dye sensitive to intracellular ROS. Further comparative investigation will aim to understand the genetic, ultrastructural and physiological characteristics supporting unicellular versus multicellular coenobial morphology, and the ability of representatives in the Scenedesmaceae to colonize ecologically diverse, even extreme, habitats.},
doi = {10.1242/jcs.212233},
journal = {Journal of Cell Science},
number = 7,
volume = 131,
place = {United Kingdom},
year = {2018},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.1242/jcs.212233

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