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Title: Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products

Abstract

Methane can be converted to triose dihydroxyacetone (DHA) by chemical processes with formaldehyde as an intermediate. Carbon dioxide, a by-product of various industries including ethanol/butanol biorefineries, can also be converted to formaldehyde and then to DHA. DHA, upon entry into a cell and phosphorylation to DHA-3-phosphate, enters the glycolytic pathway and can be fermented to any one of several products. However, DHA is inhibitory to microbes due to its chemical interaction with cellular components. Fermentation of DHA to d -lactate by Escherichia coli strain TG113 was inefficient, and growth was inhibited by 30 g⋅L −1 DHA. An ATP-dependent DHA kinase from Klebsiella oxytoca (pDC117d) permitted growth of strain TG113 in a medium with 30 g⋅L −1 DHA, and in a fed-batch fermentation the d -lactate titer of TG113(pDC117d) was 580 ± 21 mM at a yield of 0.92 g⋅g −1 DHA fermented. Klebsiella variicola strain LW225, with a higher glucose flux than E. coli , produced 811 ± 26 mM d -lactic acid at an average volumetric productivity of 2.0 g −1 ⋅L −1 ⋅h −1 . Fermentation of DHA required a balance between transport of the triose and utilization by the microorganism. Using other engineered E. coli strains, wemore » also fermented DHA to succinic acid and ethanol, demonstrating the potential of converting CH 4 and CO 2 to value-added chemicals and fuels by a combination of chemical/biological processes.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B); USDOE Office of Policy and International Affairs (PO)
OSTI Identifier:
1432427
Alternate Identifier(s):
OSTI ID: 1540286
Grant/Contract Number:  
PI0000031
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 115 Journal Issue: 17; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology; Other Topics; dihydroxyacetone; fermentation; methane; lactic acid; ethanol

Citation Formats

Wang, Liang, Chauliac, Diane, Rhee, Mun Su, Panneerselvam, Anushadevi, Ingram, Lonnie O., and Shanmugam, K. T. Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products. United States: N. p., 2018. Web. doi:10.1073/pnas.1801002115.
Wang, Liang, Chauliac, Diane, Rhee, Mun Su, Panneerselvam, Anushadevi, Ingram, Lonnie O., & Shanmugam, K. T. Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products. United States. doi:10.1073/pnas.1801002115.
Wang, Liang, Chauliac, Diane, Rhee, Mun Su, Panneerselvam, Anushadevi, Ingram, Lonnie O., and Shanmugam, K. T. Mon . "Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products". United States. doi:10.1073/pnas.1801002115.
@article{osti_1432427,
title = {Fermentation of dihydroxyacetone by engineered Escherichia coli and Klebsiella variicola to products},
author = {Wang, Liang and Chauliac, Diane and Rhee, Mun Su and Panneerselvam, Anushadevi and Ingram, Lonnie O. and Shanmugam, K. T.},
abstractNote = {Methane can be converted to triose dihydroxyacetone (DHA) by chemical processes with formaldehyde as an intermediate. Carbon dioxide, a by-product of various industries including ethanol/butanol biorefineries, can also be converted to formaldehyde and then to DHA. DHA, upon entry into a cell and phosphorylation to DHA-3-phosphate, enters the glycolytic pathway and can be fermented to any one of several products. However, DHA is inhibitory to microbes due to its chemical interaction with cellular components. Fermentation of DHA to d -lactate by Escherichia coli strain TG113 was inefficient, and growth was inhibited by 30 g⋅L −1 DHA. An ATP-dependent DHA kinase from Klebsiella oxytoca (pDC117d) permitted growth of strain TG113 in a medium with 30 g⋅L −1 DHA, and in a fed-batch fermentation the d -lactate titer of TG113(pDC117d) was 580 ± 21 mM at a yield of 0.92 g⋅g −1 DHA fermented. Klebsiella variicola strain LW225, with a higher glucose flux than E. coli , produced 811 ± 26 mM d -lactic acid at an average volumetric productivity of 2.0 g −1 ⋅L −1 ⋅h −1 . Fermentation of DHA required a balance between transport of the triose and utilization by the microorganism. Using other engineered E. coli strains, we also fermented DHA to succinic acid and ethanol, demonstrating the potential of converting CH 4 and CO 2 to value-added chemicals and fuels by a combination of chemical/biological processes.},
doi = {10.1073/pnas.1801002115},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 17,
volume = 115,
place = {United States},
year = {2018},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1073/pnas.1801002115

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