The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis
Abstract
Light utilization is finely tuned in photosynthetic organisms to prevent cellular damage. The dissipation of excess absorbed light energy, a process termed nonphotochemical quenching (NPQ), plays an important role in photoprotection. Little is known about the sustained or slowly reversible form(s) of NPQ and whether they are photoprotective, in part due to the lack of mutants. The Arabidopsis thaliana suppressor of quenching1 (soq1) mutant exhibits enhanced sustained NPQ, which we term qH. To identify molecular players involved in qH, we screened for suppressors of soq1 and isolated mutants affecting either chlorophyllide a oxygenase or the chloroplastic lipocalin, now renamed plastid lipocalin (LCNP). An analysis of the mutants confirmed that qH is localized to the peripheral antenna (LHCII) of photosystem II and demonstrated that LCNP is required for qH, either directly (by forming NPQ sites) or indirectly (by modifying the LHCII membrane environment). qH operates under stress conditions such as cold and high light and is photoprotective, as it reduces lipid peroxidation levels.We propose that, under stress conditions, LCNP protects the thylakoid membrane by enabling sustained NPQ in LHCII, thereby preventing singlet oxygen stress.
- Authors:
-
[1];
[2];
[2];
[3];
[3];
[4]
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
- Aix-Marseille Univ. and CEA/CNRS UMR, Marseille (France). Plant Biology and Environmental Microbiology and Lab. of Molecular Ecophysiology of Plants (LEMP)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology and Howard Hughes Medical Inst.
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; Cornell Univ., Ithaca, NY (United States); Univ. of California, Berkeley, CA (United States); National Institutes of Health (NIH)
- OSTI Identifier:
- 1413005
- Alternate Identifier(s):
- OSTI ID: 1432241
- Grant/Contract Number:
- AC02-05CH11231; S10RR029668; S10RR027303; GM007127
- Resource Type:
- Published Article
- Journal Name:
- Plant Cell
- Additional Journal Information:
- Journal Name: Plant Cell Journal Volume: 30 Journal Issue: 1; Journal ID: ISSN 1040-4651
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Malnoe, Alizee, Schultink, Alex, Shahrasbi, Sanya, Rumeau, Dominique, Havaux, Michel, and Niyogi, Krishna K. The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis. United States: N. p., 2017.
Web. doi:10.1105/tpc.17.00536.
Malnoe, Alizee, Schultink, Alex, Shahrasbi, Sanya, Rumeau, Dominique, Havaux, Michel, & Niyogi, Krishna K. The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis. United States. https://doi.org/10.1105/tpc.17.00536
Malnoe, Alizee, Schultink, Alex, Shahrasbi, Sanya, Rumeau, Dominique, Havaux, Michel, and Niyogi, Krishna K. Tue .
"The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis". United States. https://doi.org/10.1105/tpc.17.00536.
@article{osti_1413005,
title = {The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis},
author = {Malnoe, Alizee and Schultink, Alex and Shahrasbi, Sanya and Rumeau, Dominique and Havaux, Michel and Niyogi, Krishna K.},
abstractNote = {Light utilization is finely tuned in photosynthetic organisms to prevent cellular damage. The dissipation of excess absorbed light energy, a process termed nonphotochemical quenching (NPQ), plays an important role in photoprotection. Little is known about the sustained or slowly reversible form(s) of NPQ and whether they are photoprotective, in part due to the lack of mutants. The Arabidopsis thaliana suppressor of quenching1 (soq1) mutant exhibits enhanced sustained NPQ, which we term qH. To identify molecular players involved in qH, we screened for suppressors of soq1 and isolated mutants affecting either chlorophyllide a oxygenase or the chloroplastic lipocalin, now renamed plastid lipocalin (LCNP). An analysis of the mutants confirmed that qH is localized to the peripheral antenna (LHCII) of photosystem II and demonstrated that LCNP is required for qH, either directly (by forming NPQ sites) or indirectly (by modifying the LHCII membrane environment). qH operates under stress conditions such as cold and high light and is photoprotective, as it reduces lipid peroxidation levels.We propose that, under stress conditions, LCNP protects the thylakoid membrane by enabling sustained NPQ in LHCII, thereby preventing singlet oxygen stress.},
doi = {10.1105/tpc.17.00536},
journal = {Plant Cell},
number = 1,
volume = 30,
place = {United States},
year = {Tue Dec 12 00:00:00 EST 2017},
month = {Tue Dec 12 00:00:00 EST 2017}
}
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https://doi.org/10.1105/tpc.17.00536
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