Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation
Abstract
26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for granule assembly, not only influences the rate of proteasome degradation, but also impacts cell viability upon recovery from carbon starvation. PSG formation and concomitant protection against proteaphagy also occurs in Arabidopsis , suggesting that PSGs represent an evolutionarily conserved cache of proteasomes that can be rapidly re-mobilized based on energy availability.
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Institutes of Health )NIH)
- OSTI Identifier:
- 1432090
- Alternate Identifier(s):
- OSTI ID: 1432092; OSTI ID: 1509811
- Grant/Contract Number:
- FG02-88ER13968
- Resource Type:
- Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 7; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Marshall, Richard S., and Vierstra, Richard D. Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation. United States: N. p., 2018.
Web. doi:10.7554/eLife.34532.
Marshall, Richard S., & Vierstra, Richard D. Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation. United States. https://doi.org/10.7554/eLife.34532
Marshall, Richard S., and Vierstra, Richard D. Fri .
"Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation". United States. https://doi.org/10.7554/eLife.34532.
@article{osti_1432090,
title = {Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation},
author = {Marshall, Richard S. and Vierstra, Richard D.},
abstractNote = {26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for granule assembly, not only influences the rate of proteasome degradation, but also impacts cell viability upon recovery from carbon starvation. PSG formation and concomitant protection against proteaphagy also occurs in Arabidopsis , suggesting that PSGs represent an evolutionarily conserved cache of proteasomes that can be rapidly re-mobilized based on energy availability.},
doi = {10.7554/eLife.34532},
journal = {eLife},
number = ,
volume = 7,
place = {United States},
year = {2018},
month = {4}
}
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