RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana
Abstract
Summary Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBis CO ) catalyzes the reaction between gaseous carbon dioxide ( CO 2 ) and ribulose‐1,5‐bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBis CO is still emerging. In bacteria and in the C4 plant Zea mays , a protein with distant homology to p terin‐4α‐ c arbinolamine d ehydratase ( PCD ) has recently been shown to be involved in RuBis CO assembly. However, studies of the homologous PCD ‐like protein ( RAF 2, RuBis CO assembly factor 2) in the C3 plant Arabidopsis thaliana ( A . thaliana ) have so far focused on its role in hormone and stress signaling. We investigated whether A . thaliana RAF 2 is also involved in RuBis CO assembly. We localized RAF 2 to the soluble chloroplast stroma and demonstrated that raf2 A . thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBis CO . We concluded that the RAF 2 protein is probably involved in RuBis CO assembly in the C3 plant A . thaliana .
- Authors:
-
- Biophysics of Photosynthesis VU University Amsterdam Amsterdam The Netherlands, Department of Chemistry and Biochemistry and Institute for Genomics and Proteomics UCLA Los Angeles CA USA
- Biophysics of Photosynthesis VU University Amsterdam Amsterdam The Netherlands
- Department of Chemistry and Biochemistry and Institute for Genomics and Proteomics UCLA Los Angeles CA USA
- School of Molecular Sciences and Center for Bioenergy and Photosynthesis Arizona State University Tempe AZ USA
- Department of Energy Plant Research Laboratory Department of Biochemistry and Molecular Biology Michigan State University East Lansing USA
- Horticulture and Product Physiology Group Wageningen University Wageningen The Netherlands
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1432069
- Grant/Contract Number:
- DE‐FG02‐09‐ER16123
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 94 Journal Issue: 1; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Fristedt, Rikard, Hu, Chen, Wheatley, Nicole, Roy, Laura M., Wachter, Rebekka M., Savage, Linda, Harbinson, Jeremy, Kramer, David M., Merchant, Sabeeha S., Yeates, Todd, and Croce, Roberta. RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana. United Kingdom: N. p., 2018.
Web. doi:10.1111/tpj.13849.
Fristedt, Rikard, Hu, Chen, Wheatley, Nicole, Roy, Laura M., Wachter, Rebekka M., Savage, Linda, Harbinson, Jeremy, Kramer, David M., Merchant, Sabeeha S., Yeates, Todd, & Croce, Roberta. RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana. United Kingdom. https://doi.org/10.1111/tpj.13849
Fristedt, Rikard, Hu, Chen, Wheatley, Nicole, Roy, Laura M., Wachter, Rebekka M., Savage, Linda, Harbinson, Jeremy, Kramer, David M., Merchant, Sabeeha S., Yeates, Todd, and Croce, Roberta. Mon .
"RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana". United Kingdom. https://doi.org/10.1111/tpj.13849.
@article{osti_1432069,
title = {RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana},
author = {Fristedt, Rikard and Hu, Chen and Wheatley, Nicole and Roy, Laura M. and Wachter, Rebekka M. and Savage, Linda and Harbinson, Jeremy and Kramer, David M. and Merchant, Sabeeha S. and Yeates, Todd and Croce, Roberta},
abstractNote = {Summary Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBis CO ) catalyzes the reaction between gaseous carbon dioxide ( CO 2 ) and ribulose‐1,5‐bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBis CO is still emerging. In bacteria and in the C4 plant Zea mays , a protein with distant homology to p terin‐4α‐ c arbinolamine d ehydratase ( PCD ) has recently been shown to be involved in RuBis CO assembly. However, studies of the homologous PCD ‐like protein ( RAF 2, RuBis CO assembly factor 2) in the C3 plant Arabidopsis thaliana ( A . thaliana ) have so far focused on its role in hormone and stress signaling. We investigated whether A . thaliana RAF 2 is also involved in RuBis CO assembly. We localized RAF 2 to the soluble chloroplast stroma and demonstrated that raf2 A . thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBis CO . We concluded that the RAF 2 protein is probably involved in RuBis CO assembly in the C3 plant A . thaliana .},
doi = {10.1111/tpj.13849},
journal = {The Plant Journal},
number = 1,
volume = 94,
place = {United Kingdom},
year = {Mon Mar 12 00:00:00 EDT 2018},
month = {Mon Mar 12 00:00:00 EDT 2018}
}
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