skip to main content

DOE PAGESDOE PAGES

Title: Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans

Background: Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an Aspergillus nidulans strain that releases but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase (hxkA) and glucokinase (glkA) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in creA, the repressor involved in regulating preferential use of different carbon catabolic pathways. Results: Phenotypic analysis revealed reduced growth for the hxkA1 glkA4 mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The creAΔ4 mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the hxkA/glkA mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat branmore » as a major carbon source to support growth. Conclusion: Blocking the direct entry of hexoses to glycolysis activates alternative metabolic conversion of these sugars in A. nidulans during growth on plant biomass, but also upregulates conversion of other sugars, such as pentoses.« less
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [3] ;  [3] ; ORCiD logo [1]
  1. Univ. of Utrecht (Netherlands)
  2. Univ. of Utrecht (Netherlands); Fungal Genetics and Technology Consultancy, Wageningen (Netherlands)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-132841
Journal ID: ISSN 1471-2164; KP1601010
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 19; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1431406

Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, Maria Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., and de Vries, Ronald P.. Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans. United States: N. p., Web. doi:10.1186/s12864-018-4609-x.
Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, Maria Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., & de Vries, Ronald P.. Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans. United States. doi:10.1186/s12864-018-4609-x.
Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, Maria Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., and de Vries, Ronald P.. 2018. "Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans". United States. doi:10.1186/s12864-018-4609-x. https://www.osti.gov/servlets/purl/1431406.
@article{osti_1431406,
title = {Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans},
author = {Khosravi, Claire and Battaglia, Evy and Kun, Roland S. and Dalhuijsen, Sacha and Visser, Jaap and Aguilar-Pontes, Maria Victoria and Zhou, Miaomiao and Heyman, Heino M. and Kim, Young-Mo and Baker, Scott E. and de Vries, Ronald P.},
abstractNote = {Background: Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an Aspergillus nidulans strain that releases but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase (hxkA) and glucokinase (glkA) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in creA, the repressor involved in regulating preferential use of different carbon catabolic pathways. Results: Phenotypic analysis revealed reduced growth for the hxkA1 glkA4 mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The creAΔ4 mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the hxkA/glkA mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat bran as a major carbon source to support growth. Conclusion: Blocking the direct entry of hexoses to glycolysis activates alternative metabolic conversion of these sugars in A. nidulans during growth on plant biomass, but also upregulates conversion of other sugars, such as pentoses.},
doi = {10.1186/s12864-018-4609-x},
journal = {BMC Genomics},
number = 1,
volume = 19,
place = {United States},
year = {2018},
month = {3}
}

Works referenced in this record:

Mapping and quantifying mammalian transcriptomes by RNA-Seq
journal, May 2008
  • Mortazavi, Ali; Williams, Brian A.; McCue, Kenneth
  • Nature Methods, Vol. 5, Issue 7, p. 621-628
  • DOI: 10.1038/nmeth.1226