Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans
Abstract
Background: Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an Aspergillus nidulans strain that releases but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase (hxkA) and glucokinase (glkA) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in creA, the repressor involved in regulating preferential use of different carbon catabolic pathways. Results: Phenotypic analysis revealed reduced growth for the hxkA1 glkA4 mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The creAΔ4 mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the hxkA/glkA mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat branmore »
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1618548
- Alternate Identifier(s):
- OSTI ID: 1431406
- Report Number(s):
- PNNL-SA-132841
Journal ID: ISSN 1471-2164; 214; PII: 4609
- Grant/Contract Number:
- SC0008744; AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- BMC Genomics
- Additional Journal Information:
- Journal Name: BMC Genomics Journal Volume: 19 Journal Issue: 1; Journal ID: ISSN 1471-2164
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, María Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., and de Vries, Ronald P. Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans. United Kingdom: N. p., 2018.
Web. doi:10.1186/s12864-018-4609-x.
Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, María Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., & de Vries, Ronald P. Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans. United Kingdom. https://doi.org/10.1186/s12864-018-4609-x
Khosravi, Claire, Battaglia, Evy, Kun, Roland S., Dalhuijsen, Sacha, Visser, Jaap, Aguilar-Pontes, María Victoria, Zhou, Miaomiao, Heyman, Heino M., Kim, Young-Mo, Baker, Scott E., and de Vries, Ronald P. Thu .
"Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans". United Kingdom. https://doi.org/10.1186/s12864-018-4609-x.
@article{osti_1618548,
title = {Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans},
author = {Khosravi, Claire and Battaglia, Evy and Kun, Roland S. and Dalhuijsen, Sacha and Visser, Jaap and Aguilar-Pontes, María Victoria and Zhou, Miaomiao and Heyman, Heino M. and Kim, Young-Mo and Baker, Scott E. and de Vries, Ronald P.},
abstractNote = {Background: Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an Aspergillus nidulans strain that releases but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase (hxkA) and glucokinase (glkA) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in creA, the repressor involved in regulating preferential use of different carbon catabolic pathways. Results: Phenotypic analysis revealed reduced growth for the hxkA1 glkA4 mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The creAΔ4 mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the hxkA/glkA mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat bran as a major carbon source to support growth. Conclusion: Blocking the direct entry of hexoses to glycolysis activates alternative metabolic conversion of these sugars in A. nidulans during growth on plant biomass, but also upregulates conversion of other sugars, such as pentoses.},
doi = {10.1186/s12864-018-4609-x},
journal = {BMC Genomics},
number = 1,
volume = 19,
place = {United Kingdom},
year = {Thu Mar 22 00:00:00 EDT 2018},
month = {Thu Mar 22 00:00:00 EDT 2018}
}
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https://doi.org/10.1186/s12864-018-4609-x
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