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Title: Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications

Sweet sorghum is a promising crop for a warming, drying African climate, and basic information is lacking on conversion pathways for its lignocellulosic residues (bagasse). Brown rot wood-decomposer fungi use carbohydrate-selective pathways that, when assessed on sorghum, a grass substrate, can yield information relevant to both plant biomass conversion and fungal biology. In testing sorghum decomposition by brown rot fungi ( Gloeophyllum trabeum , Serpula lacrymans ), we found that G. trabeum readily degraded sorghum, removing xylan prior to removing glucan. Serpula lacrymans , conversely, caused little decomposition. Ergosterol (fungal biomarker) and protein levels were similar for both fungi, but S. lacrymans produced nearly 4x lower polysaccharide-degrading enzyme specific activity on sorghum than G. trabeum , perhaps a symptom of starvation. Linking this information to genome comparisons including other brown rot fungi known to have a similar issue regarding decomposing grasses (Postia placenta, Fomitopsis pinicola) suggested that a lack of CE 1 feruloyl esterases as well as low xylanase activity in S. lacrymans (3x lower than in G. trabeum ) may hinder S. lacrymans , P. placenta, and F. pinicola when degrading grass substrates. These results indicate variability in brown rot mechanisms, which may stem from a differing ability tomore » degrade certain lignin-carbohydrate complexes.« less
Authors:
ORCiD logo [1] ;  [2] ; ORCiD logo [3]
  1. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 2004 Folwell Ave. St. Paul, MN 55108, USA
  2. Agricultural Research Council of South Africa (ARC-Infruitec/Nietvoorbij), Private Bag X5026, Stellenbosch 7599, South Africa, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa
  3. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 2004 Folwell Ave. St. Paul, MN 55108, USA, Department of Plant and Microbial Biology, University of Minnesota, 1500 Gortner Ave. St. Paul, MN 55108, USA
Publication Date:
Grant/Contract Number:
SC0004012; SC0012742
Type:
Published Article
Journal Name:
International Journal of Microbiology
Additional Journal Information:
Journal Volume: 2018; Related Information: CHORUS Timestamp: 2018-04-05 18:16:15; Journal ID: ISSN 1687-918X
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English
OSTI Identifier:
1431388