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Title: Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications

Abstract

Sweet sorghum is a promising crop for a warming, drying African climate, and basic information is lacking on conversion pathways for its lignocellulosic residues (bagasse). Brown rot wood-decomposer fungi use carbohydrate-selective pathways that, when assessed on sorghum, a grass substrate, can yield information relevant to both plant biomass conversion and fungal biology. In testing sorghum decomposition by brown rot fungi ( Gloeophyllum trabeum , Serpula lacrymans ), we found that G. trabeum readily degraded sorghum, removing xylan prior to removing glucan. Serpula lacrymans , conversely, caused little decomposition. Ergosterol (fungal biomarker) and protein levels were similar for both fungi, but S. lacrymans produced nearly 4x lower polysaccharide-degrading enzyme specific activity on sorghum than G. trabeum , perhaps a symptom of starvation. Linking this information to genome comparisons including other brown rot fungi known to have a similar issue regarding decomposing grasses (Postia placenta, Fomitopsis pinicola) suggested that a lack of CE 1 feruloyl esterases as well as low xylanase activity in S. lacrymans (3x lower than in G. trabeum ) may hinder S. lacrymans , P. placenta, and F. pinicola when degrading grass substrates. These results indicate variability in brown rot mechanisms, which may stem from a differing ability tomore » degrade certain lignin-carbohydrate complexes.« less

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3]
  1. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 2004 Folwell Ave. St. Paul, MN 55108, USA
  2. Agricultural Research Council of South Africa (ARC-Infruitec/Nietvoorbij), Private Bag X5026, Stellenbosch 7599, South Africa, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa
  3. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 2004 Folwell Ave. St. Paul, MN 55108, USA, Department of Plant and Microbial Biology, University of Minnesota, 1500 Gortner Ave. St. Paul, MN 55108, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1431388
Grant/Contract Number:  
SC0004012; SC0012742
Resource Type:
Published Article
Journal Name:
International Journal of Microbiology
Additional Journal Information:
Journal Name: International Journal of Microbiology Journal Volume: 2018; Journal ID: ISSN 1687-918X
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Presley, Gerald N., Ndimba, Bongani K., and Schilling, Jonathan S. Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications. Country unknown/Code not available: N. p., 2018. Web. doi:10.1155/2018/4961726.
Presley, Gerald N., Ndimba, Bongani K., & Schilling, Jonathan S. Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications. Country unknown/Code not available. doi:10.1155/2018/4961726.
Presley, Gerald N., Ndimba, Bongani K., and Schilling, Jonathan S. Mon . "Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications". Country unknown/Code not available. doi:10.1155/2018/4961726.
@article{osti_1431388,
title = {Brown Rot-Type Fungal Decomposition of Sorghum Bagasse: Variable Success and Mechanistic Implications},
author = {Presley, Gerald N. and Ndimba, Bongani K. and Schilling, Jonathan S.},
abstractNote = {Sweet sorghum is a promising crop for a warming, drying African climate, and basic information is lacking on conversion pathways for its lignocellulosic residues (bagasse). Brown rot wood-decomposer fungi use carbohydrate-selective pathways that, when assessed on sorghum, a grass substrate, can yield information relevant to both plant biomass conversion and fungal biology. In testing sorghum decomposition by brown rot fungi ( Gloeophyllum trabeum , Serpula lacrymans ), we found that G. trabeum readily degraded sorghum, removing xylan prior to removing glucan. Serpula lacrymans , conversely, caused little decomposition. Ergosterol (fungal biomarker) and protein levels were similar for both fungi, but S. lacrymans produced nearly 4x lower polysaccharide-degrading enzyme specific activity on sorghum than G. trabeum , perhaps a symptom of starvation. Linking this information to genome comparisons including other brown rot fungi known to have a similar issue regarding decomposing grasses (Postia placenta, Fomitopsis pinicola) suggested that a lack of CE 1 feruloyl esterases as well as low xylanase activity in S. lacrymans (3x lower than in G. trabeum ) may hinder S. lacrymans , P. placenta, and F. pinicola when degrading grass substrates. These results indicate variability in brown rot mechanisms, which may stem from a differing ability to degrade certain lignin-carbohydrate complexes.},
doi = {10.1155/2018/4961726},
journal = {International Journal of Microbiology},
number = ,
volume = 2018,
place = {Country unknown/Code not available},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2018/4961726

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Works referenced in this record:

Life-cycle energy use and greenhouse gas emissions of production of bioethanol from sorghum in the United States
journal, January 2013

  • Cai, Hao; Dunn, Jennifer B.; Wang, Zhichao
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-141

Biofuels in sub-Sahara Africa: Drivers, impacts and priority policy areas
journal, May 2015

  • Gasparatos, A.; von Maltitz, G. P.; Johnson, F. X.
  • Renewable and Sustainable Energy Reviews, Vol. 45
  • DOI: 10.1016/j.rser.2015.02.006

Analysis of Character Correlations Among Wood Decay Mechanisms, Mating Systems, and Substrate Ranges in Homobasidiomycetes
journal, March 2001


Role of carbon source in the shift from oxidative to hydrolytic wood decomposition by Postia placenta
journal, September 2017


The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes
journal, June 2012


Various pretreatments of lignocellulosics
journal, January 2016


Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta
journal, September 2016

  • Zhang, Jiwei; Presley, Gerald N.; Hammel, Kenneth E.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 39
  • DOI: 10.1073/pnas.1608454113

Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase
journal, February 2013


Production of fungal xylanases
journal, November 1996


Using a grass substrate to compare decay among two clades of brown rot fungi
journal, August 2013

  • Kaffenberger, Justin T.; Schilling, Jonathan S.
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 19
  • DOI: 10.1007/s00253-013-5142-0

Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose
journal, March 2012

  • Zhao, Xuebing; Zhang, Lihua; Liu, Dehua
  • Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 4
  • DOI: 10.1002/bbb.1331

Oxalate regulation by two brown rot fungi decaying oxalate-amended and non-amended wood
journal, November 2005

  • Schilling, Jonathan S.; Jellison, Jody
  • Holzforschung, Vol. 59, Issue 6
  • DOI: 10.1515/HF.2005.109

The carbon starvation response of Aspergillus niger during submerged cultivation: Insights from the transcriptome and secretome
journal, January 2012

  • Nitsche, Benjamin M.; Jørgensen, Thomas R.; Akeroyd, Michiel
  • BMC Genomics, Vol. 13, Issue 1
  • DOI: 10.1186/1471-2164-13-380

Are we ready to cultivate sweet sorghum as a bioenergy feedstock? A review on field management practices
journal, May 2012


Climate change impacts in Sub-Saharan Africa: from physical changes to their social repercussions
journal, January 2016

  • Serdeczny, Olivia; Adams, Sophie; Baarsch, Florent
  • Regional Environmental Change, Vol. 17, Issue 6
  • DOI: 10.1007/s10113-015-0910-2

Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies
journal, September 2009


Biofuels Possibilities in the Southern Africa Context
journal, September 2010

  • Diaz-Chavez, R.; Jamieson, C.
  • Journal of Biobased Materials and Bioenergy, Vol. 4, Issue 3
  • DOI: 10.1166/jbmb.2010.1090

Towards enzymatic breakdown of complex plant xylan structures: State of the art
journal, November 2016


Sweet sorghum as a bioenergy crop: Literature review
journal, May 2014


The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus
journal, March 2015

  • Ellström, Magnus; Shah, Firoz; Johansson, Tomas
  • FEMS Microbiology Ecology, Vol. 91, Issue 4
  • DOI: 10.1093/femsec/fiv027

Distinct Growth and Secretome Strategies for Two Taxonomically Divergent Brown Rot Fungi
journal, January 2017

  • Presley, Gerald N.; Schilling, Jonathan S.
  • Applied and Environmental Microbiology, Vol. 83, Issue 7
  • DOI: 10.1128/AEM.02987-16

Measurement of cellulase activities
journal, January 1987


Lignocellulose modifications by brown rot fungi and their effects, as pretreatments, on cellulolysis
journal, July 2012


A non-modular type B feruloyl esterase from Neurospora crassa exhibits concentration-dependent substrate inhibition
journal, March 2003

  • Crepin, Valerie F.; Faulds, Craig B.; Connerton, Ian F.
  • Biochemical Journal, Vol. 370, Issue 2
  • DOI: 10.1042/bj20020917

Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi
journal, June 2014

  • Riley, Robert; Salamov, Asaf A.; Brown, Daren W.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 27
  • DOI: 10.1073/pnas.1400592111

Unique aspects of the grass cell wall
journal, June 2008