Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption
Abstract
Interactions between silica and proteins are crucial for the formation of biosilica and the production of novel functional hybrid materials for a range of industrial applications. The proteins control both precipitation pathway and the properties of the resulting silica–organic composites. Here, we present data on the formation of silica–lysozyme composites through two different synthesis approaches (co-precipitation vs. adsorption) and show that the chemical and structural properties of these composites, when analyzed using a combination of synchrotron-based scattering (total scattering and small-angle X-ray scattering), spectroscopic, electron microscopy, and potentiometric methods vary dramatically. We document that while lysozyme was not incorporated into nor did its presence alter the molecular structure of silica, it strongly enhanced the aggregation of silica particles due to electrostatic and potentially hydrophobic interactions, leading to the formation of composites with characteristics differing from pure silica. The differences increased with increasing lysozyme content for both synthesis approaches. Yet, the absolute changes differ substantially between the two sets of composites, as lysozyme did not just affect aggregation during co-precipitation but also particle growth and likely polymerization during co-precipitation. Our results improve the fundamental understanding of how organic macromolecules interact with dissolved and nanoparticulate silica and how these interactions control themore »
- Authors:
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE Office of Science (SC); European Commission (EC); Natural Environment Research Council (NERC); Danish Council for Independent Research (DFF)
- OSTI Identifier:
- 1431135
- Alternate Identifier(s):
- OSTI ID: 1761446
- Grant/Contract Number:
- NE/J008745/1; AC02-06CH11357
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Materials
- Additional Journal Information:
- Journal Name: Frontiers in Materials Journal Volume: 5; Journal ID: ISSN 2296-8016
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; silica; lysozyme; co-precipitation; adsorption; composite materials; hybrid material; biosilica
Citation Formats
van den Heuvel, Daniela B., Stawski, Tomasz M., Tobler, Dominique J., Wirth, Richard, Peacock, Caroline L., and Benning, Liane G. Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption. Switzerland: N. p., 2018.
Web. doi:10.3389/fmats.2018.00019.
van den Heuvel, Daniela B., Stawski, Tomasz M., Tobler, Dominique J., Wirth, Richard, Peacock, Caroline L., & Benning, Liane G. Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption. Switzerland. https://doi.org/10.3389/fmats.2018.00019
van den Heuvel, Daniela B., Stawski, Tomasz M., Tobler, Dominique J., Wirth, Richard, Peacock, Caroline L., and Benning, Liane G. Tue .
"Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption". Switzerland. https://doi.org/10.3389/fmats.2018.00019.
@article{osti_1431135,
title = {Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption},
author = {van den Heuvel, Daniela B. and Stawski, Tomasz M. and Tobler, Dominique J. and Wirth, Richard and Peacock, Caroline L. and Benning, Liane G.},
abstractNote = {Interactions between silica and proteins are crucial for the formation of biosilica and the production of novel functional hybrid materials for a range of industrial applications. The proteins control both precipitation pathway and the properties of the resulting silica–organic composites. Here, we present data on the formation of silica–lysozyme composites through two different synthesis approaches (co-precipitation vs. adsorption) and show that the chemical and structural properties of these composites, when analyzed using a combination of synchrotron-based scattering (total scattering and small-angle X-ray scattering), spectroscopic, electron microscopy, and potentiometric methods vary dramatically. We document that while lysozyme was not incorporated into nor did its presence alter the molecular structure of silica, it strongly enhanced the aggregation of silica particles due to electrostatic and potentially hydrophobic interactions, leading to the formation of composites with characteristics differing from pure silica. The differences increased with increasing lysozyme content for both synthesis approaches. Yet, the absolute changes differ substantially between the two sets of composites, as lysozyme did not just affect aggregation during co-precipitation but also particle growth and likely polymerization during co-precipitation. Our results improve the fundamental understanding of how organic macromolecules interact with dissolved and nanoparticulate silica and how these interactions control the formation pathway of silica–organic composites from sodium silicate solutions, a widely available and cheap starting material.},
doi = {10.3389/fmats.2018.00019},
journal = {Frontiers in Materials},
number = ,
volume = 5,
place = {Switzerland},
year = {Tue Apr 03 00:00:00 EDT 2018},
month = {Tue Apr 03 00:00:00 EDT 2018}
}
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https://doi.org/10.3389/fmats.2018.00019
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Cited by: 12 works
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