skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation

Abstract

Alkaline deacetylation has emerged as a promising chemistry for pretreatments performed prior to enzymatic saccharification of lignocellulosic biomass. This process avoids complex pressurized reactors and opens new opportunities for lignin covalorization. In this work, we evaluate the chemical and morphological response of sugar cane bagasse and straw submitted to alkaline treatments. Alkaline solutions for deacetylation (0.4% w/w NaOH, 70 degrees C, 3 h) as well as proximal conditions (0.1-0.7% NaOH, 55-85 degrees C, 1-5 h) chosen by 23 experimental design were evaluated. The deacetylation treatment removes ~90% of the acetyl groups and 20-30% of the lignin from both bagasse and straw, while removal of ~20% of the xylan and glucan is observed in straw, but not in bagasse. Considering nanoscale structural alterations, neither cellulose cocrystallization (evaluated by X-ray diffraction) nor formation of lignin aggregates (evaluated by thermoporometric signature) are observed after the alkaline conditions, in contrast to observations after hydrothermal treatments. Furthermore, calorimetric thermoporometry as well as scanning and transmission electron microscopies show substantial introduction of nanoscale porosity and loosening of the tissue and cell wall structures, indicating desirable mechanical weakening and gains in enzyme accessibility. These results provide fundamental and practical knowledge for biorefineries based on alkaline deacetylation ofmore » sugar cane bagasse and straw.« less

Authors:
 [1];  [2];  [3];  [4];  [4];  [2]
  1. Brazilian Center for Research in Energy and Materials (CNPEM), Sao Paulo (Brazil); Univ.of Sao Paulo (Brazil)
  2. Brazilian Center for Research in Energy and Materials (CNPEM), Sao Paulo (Brazil)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  4. Univ.of Sao Paulo (Brazil)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1430818
Report Number(s):
NREL/JA-2700-71218
Journal ID: ISSN 2168-0485
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
ACS Sustainable Chemistry & Engineering
Additional Journal Information:
Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 2168-0485
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bagasse; deacetylation; pretreatment; straw; sugar cane

Citation Formats

Lima, Cleilton S., Rabelo, Sarita C., Ciesielski, Peter N., Roberto, Ines C., Rocha, George J. M., and Driemeier, Carlos. Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation. United States: N. p., 2018. Web. https://doi.org/10.1021/acssuschemeng.7b04158.
Lima, Cleilton S., Rabelo, Sarita C., Ciesielski, Peter N., Roberto, Ines C., Rocha, George J. M., & Driemeier, Carlos. Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation. United States. https://doi.org/10.1021/acssuschemeng.7b04158
Lima, Cleilton S., Rabelo, Sarita C., Ciesielski, Peter N., Roberto, Ines C., Rocha, George J. M., and Driemeier, Carlos. Wed . "Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation". United States. https://doi.org/10.1021/acssuschemeng.7b04158. https://www.osti.gov/servlets/purl/1430818.
@article{osti_1430818,
title = {Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation},
author = {Lima, Cleilton S. and Rabelo, Sarita C. and Ciesielski, Peter N. and Roberto, Ines C. and Rocha, George J. M. and Driemeier, Carlos},
abstractNote = {Alkaline deacetylation has emerged as a promising chemistry for pretreatments performed prior to enzymatic saccharification of lignocellulosic biomass. This process avoids complex pressurized reactors and opens new opportunities for lignin covalorization. In this work, we evaluate the chemical and morphological response of sugar cane bagasse and straw submitted to alkaline treatments. Alkaline solutions for deacetylation (0.4% w/w NaOH, 70 degrees C, 3 h) as well as proximal conditions (0.1-0.7% NaOH, 55-85 degrees C, 1-5 h) chosen by 23 experimental design were evaluated. The deacetylation treatment removes ~90% of the acetyl groups and 20-30% of the lignin from both bagasse and straw, while removal of ~20% of the xylan and glucan is observed in straw, but not in bagasse. Considering nanoscale structural alterations, neither cellulose cocrystallization (evaluated by X-ray diffraction) nor formation of lignin aggregates (evaluated by thermoporometric signature) are observed after the alkaline conditions, in contrast to observations after hydrothermal treatments. Furthermore, calorimetric thermoporometry as well as scanning and transmission electron microscopies show substantial introduction of nanoscale porosity and loosening of the tissue and cell wall structures, indicating desirable mechanical weakening and gains in enzyme accessibility. These results provide fundamental and practical knowledge for biorefineries based on alkaline deacetylation of sugar cane bagasse and straw.},
doi = {10.1021/acssuschemeng.7b04158},
journal = {ACS Sustainable Chemistry & Engineering},
number = 3,
volume = 6,
place = {United States},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Figures / Tables:

Figure 1 Figure 1: Infrared spectra of native and deacetylated sugar cane bagasse and straw. Flat baseline was subtracted and intensity was normalized by the maxima in the carbohydrate fingerprint region (see inset).

Save / Share:

Works referenced in this record:

Exploration of the ranges of the global potential of biomass for energy
journal, August 2003


Global biomass fuel resources
journal, December 2004


Sugarcane processing for ethanol and sugar in Brazil
journal, July 2015

  • Dias, Marina Oliveira de Souza; Maciel Filho, Rubens; Mantelatto, Paulo Eduardo
  • Environmental Development, Vol. 15
  • DOI: 10.1016/j.envdev.2015.03.004

Bioethanol from lignocelluloses: Status and perspectives in Brazil
journal, July 2010

  • Soccol, Carlos Ricardo; Vandenberghe, Luciana Porto de Souza; Medeiros, Adriane Bianchi Pedroni
  • Bioresource Technology, Vol. 101, Issue 13
  • DOI: 10.1016/j.biortech.2009.11.067

Techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons
journal, March 2017

  • Junqueira, Tassia L.; Chagas, Mateus F.; Gouveia, Vera L. R.
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0722-3

Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production
journal, February 2017

  • Menandro, Lauren Maine Santos; Cantarella, Heitor; Franco, Henrique Coutinho Junqueira
  • Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 3
  • DOI: 10.1002/bbb.1760

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


Cellulosic ethanol: status and innovation
journal, June 2017


Common processes drive the thermochemical pretreatment of lignocellulosic biomass
journal, January 2014

  • Langan, Paul; Petridis, Loukas; O'Neill, Hugh M.
  • Green Chem., Vol. 16, Issue 1
  • DOI: 10.1039/C3GC41962B

Hydrothermal processing, as an alternative for upgrading agriculture residues and marine biomass according to the biorefinery concept: A review
journal, May 2013

  • Ruiz, Héctor A.; Rodríguez-Jasso, Rosa M.; Fernandes, Bruno D.
  • Renewable and Sustainable Energy Reviews, Vol. 21
  • DOI: 10.1016/j.rser.2012.11.069

Pilot scale steam explosion and diluted sulfuric acid pretreatments: Comparative study aiming the sugarcane bagasse saccharification
journal, November 2015


Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016


The fate of lignin during hydrothermal pretreatment
journal, January 2013

  • Trajano, Heather L.; Engle, Nancy L.; Foston, Marcus
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-110

Biomass pretreatments capable of enabling lignin valorization in a biorefinery process
journal, April 2016


Cellulose co-crystallization and related phenomena occurring in hydrothermal treatment of sugarcane bagasse
journal, April 2015


Cellulose Aggregation under Hydrothermal Pretreatment Conditions
journal, July 2016

  • Silveira, Rodrigo L.; Stoyanov, Stanislav R.; Kovalenko, Andriy
  • Biomacromolecules, Vol. 17, Issue 8
  • DOI: 10.1021/acs.biomac.6b00603

Ultrastructure of steam-exploded wood
journal, January 1988

  • Donaldson, L. A.; Wong, K. K. Y.; Mackie, K. L.
  • Wood Science and Technology, Vol. 22, Issue 2
  • DOI: 10.1007/BF00355846

Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
journal, December 2008

  • Donohoe, Bryon S.; Decker, Stephen R.; Tucker, Melvin P.
  • Biotechnology and Bioengineering, Vol. 101, Issue 5
  • DOI: 10.1002/bit.21959

Lignin contributions to the nanoscale porosity of raw and treated lignocelluloses as observed by calorimetric thermoporometry
journal, April 2016


Biomass feeding for thermochemical reactors
journal, October 2012

  • Dai, Jianjun; Cui, Heping; Grace, John R.
  • Progress in Energy and Combustion Science, Vol. 38, Issue 5
  • DOI: 10.1016/j.pecs.2012.04.002

A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass
journal, January 2016


The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process
journal, January 2012

  • Chen, Xiaowen; Shekiro, Joseph; Franden, Mary Ann
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-8

A highly efficient dilute alkali deacetylation and mechanical (disc) refining process for the conversion of renewable biomass to lower cost sugars
journal, January 2014

  • Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-98

Evaluation of Brazilian Sugarcane Bagasse Characterization: An Interlaboratory Comparison Study
journal, May 2016

  • Sluiter, Justin B.; Chum, Helena; Gomes, Absai C.
  • Journal of AOAC INTERNATIONAL, Vol. 99, Issue 3
  • DOI: 10.5740/jaoacint.15-0063

CRAFS: a model to analyze two-dimensional X-ray diffraction patterns of plant cellulose
journal, July 2013


Dynamic vapor sorption and thermoporometry to probe water in celluloses
journal, May 2012


Absorption of water by polymers: analysis in terms of a simple model
journal, January 1946

  • Hailwood, A. J.; Horrobin, S.
  • Transactions of the Faraday Society, Vol. 42
  • DOI: 10.1039/tf946420b084

Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components)
journal, December 1994

  • Sene, Cfb.; McCann, M. C.; Wilson, R. H.
  • Plant Physiology, Vol. 106, Issue 4
  • DOI: 10.1104/pp.106.4.1623

Improved Xylan Hydrolysis of Corn Stover by Deacetylation with High Solids Dilute Acid Pretreatment
journal, December 2011

  • Chen, Xiaowen; Shekiro, Joseph; Elander, Rick
  • Industrial & Engineering Chemistry Research, Vol. 51, Issue 1
  • DOI: 10.1021/ie201493g

Development and characterization of a high-solids deacetylation process
journal, May 2016

  • Shekiro, Joseph; Chen, Xiaowen; Smith, Holly
  • Sustainable Chemical Processes, Vol. 4, Issue 1
  • DOI: 10.1186/s40508-016-0049-6

Alkaline deacetylation as a strategy to improve sugars recovery and ethanol production from rice straw hemicellulose and cellulose
journal, November 2017

  • Castro, Rafael Cunha de Assis; Fonseca, Bruno Guedes; dos Santos, Hilton Túlio Lima
  • Industrial Crops and Products, Vol. 106
  • DOI: 10.1016/j.indcrop.2016.08.053

Structure of cellulose and microcrystalline cellulose from various wood species, cotton and flax studied by X-ray scattering
journal, December 2009


Crystallite Width Determines Monolayer Hydration across a Wide Spectrum of Celluloses Isolated from Plants
journal, December 2012

  • Driemeier, Carlos; Bragatto, Juliano
  • The Journal of Physical Chemistry B, Vol. 117, Issue 1
  • DOI: 10.1021/jp309948h

Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates
journal, January 2011

  • Arantes, Valdeir; Saddler, Jack N.
  • Biotechnology for Biofuels, Vol. 4, Issue 1, Article No. 3
  • DOI: 10.1186/1754-6834-4-3

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


Assessment of chemical transformations in eucalyptus, sugarcane bagasse and straw during hydrothermal, dilute acid, and alkaline pretreatments
journal, October 2015


2G ethanol from the whole sugarcane lignocellulosic biomass
journal, January 2015

  • Pereira, Sandra; Maehara, Larissa; Machado, Cristina
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0224-0

Assessment of alkaline pretreatment for the production of bioethanol from eucalyptus, sugarcane bagasse and sugarcane straw
journal, December 2016

  • Carvalho, Danila Morais de; Queiroz, José Humberto de; Colodette, Jorge Luiz
  • Industrial Crops and Products, Vol. 94
  • DOI: 10.1016/j.indcrop.2016.09.069

Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1)
journal, October 2011

  • Zeng, Meijuan; Ximenes, Eduardo; Ladisch, Michael R.
  • Biotechnology and Bioengineering, Vol. 109, Issue 2
  • DOI: 10.1002/bit.23337

Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment
journal, October 2014


Acetylation of woody lignocellulose: significance and regulation
journal, January 2013

  • Pawar, Prashant Mohan-Anupama; Koutaniemi, Sanna; Tenkanen, Maija
  • Frontiers in Plant Science, Vol. 4
  • DOI: 10.3389/fpls.2013.00118

Determination of cell wall ferulic and p-coumaric acids in sugarcane bagasse
journal, November 2005


Hemicelluloses
journal, June 2010


Analysis of soluble and insoluble fractions of alkali and subcritical water treated sugarcane bagasse
journal, January 2011


Differences in the chemical structure of the lignins from sugarcane bagasse and straw
journal, October 2015


Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
journal, November 2014

  • de Oliveira, Dyoni Matias; Finger-Teixeira, Aline; Rodrigues Mota, Thatiane
  • Plant Biotechnology Journal, Vol. 13, Issue 9
  • DOI: 10.1111/pbi.12292

Multiscale deconstruction of molecular architecture in corn stover
journal, January 2014

  • Inouye, Hideyo; Zhang, Yan; Yang, Lin
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep03756

    Works referencing / citing this record:

    Intraparticle Connectivity in Sugarcane Bagasse Unveiled by Pore Network Modeling
    journal, June 2019


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.