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Title: Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to HeLa Cells

Colloidal gold nanoparticles (AuNPs) are of interest as non-toxic carriers for drug delivery owing to their advanced properties, such as extensive surface-to-volume ratio and possibilities for tailoring their charge, hydrophilicity and functionality through surface chemistries. To date, various biocompatible polymers have been used for surface decoration of AuNPs to enhance their stability, payloads capacity and cellular uptake. This study describes a facile one-step method to synthesize stable AuNPs loaded with combination of two anticancer therapeutics, -bleomycin and doxorubicin. Anticancer activities, cytotoxicity, uptake and intracellular localization of the AuNPs were demonstrated in HeLa cells. We show that the therapeutic efficacy of the nanohybrid drug was strongly enhanced by the active targeting by the nanoscale delivery system to HeLa cells with a significant decrease of the half-maximal effective drug concentration, through blockage of HeLa cancer cell cycle. These results provide rationale for further progress of AuNPs-assisted combination chemotherapy using two drugs at optimized effective concentrations which act via different mechanisms thus decreasing possibilities of development of the cancer drug resistance, reduction of systemic drug toxicity and improvement of outcomes of chemotherapy.
Authors:
 [1] ; ORCiD logo [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [6] ;  [7] ;  [1]
  1. Univ. of Electronic Science and Technology of China, Chengdu (China). Inst. of Fundamental and Frontier Science
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Division; National Univ. of Science and Technology (MISiS), Moscow (Russian Federation)
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Nanoscience and Technology Division. Center for Nanoscale Materials
  4. International Islamic Univ. Islamabad (Pakistan). Dept. of Physics
  5. Univ. of Electronic Science and Technology of China, Chengdu (China). School of Life Science and Technology
  6. Univ. of North Texas, Denton, TX (United States). Dept. of Biological Science
  7. Univ. of North Texas, Denton, TX (United States). Dept. of Physics
Publication Date:
Grant/Contract Number:
AC02-06CH11357; K3-2017-070
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Electronic Science and Technology of China, Chengdu (China); National Univ. of Science and Technology (MISiS), Moscow (Russian Federation)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Univ. of Electronic Science and Technology of China (UESTC); Ministry of Education and Science of the Russian Federation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; chemotherapy; drug delivery
OSTI Identifier:
1430708