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Title: Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse

Abstract

How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. Here, we started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightly increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.

Authors:
 [1];  [1]; ORCiD logo [2];  [1];  [1];  [3]
  1. Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Environmental and Applied Microbiology; Chinese Academy of Sciences (CAS), Sichuan (China). Environmental Microbiology Key Lab. of Sichuan Province, Chengdu Inst. of Biology
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Earth and Biological Sciences Directorate, Biological Sciences Division
  3. Fujian Agriculture and Forestry Univ., Fuzhou (China). Fujian Provincial Key Lab. of Soil Environmental Health and Regulation, College of Resources and Environment
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1430531
Report Number(s):
PNNL-SA-127737
Journal ID: ISSN 2045-2322; PII: 17736
Grant/Contract Number:  
AC0576RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Liu, Chi, Yao, Minjie, Stegen, James C., Rui, Junpeng, Li, Jiabao, and Li, Xiangzhen. Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse. United States: N. p., 2017. Web. doi:10.1038/S41598-017-17736-W.
Liu, Chi, Yao, Minjie, Stegen, James C., Rui, Junpeng, Li, Jiabao, & Li, Xiangzhen. Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse. United States. doi:10.1038/S41598-017-17736-W.
Liu, Chi, Yao, Minjie, Stegen, James C., Rui, Junpeng, Li, Jiabao, and Li, Xiangzhen. Wed . "Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse". United States. doi:10.1038/S41598-017-17736-W. https://www.osti.gov/servlets/purl/1430531.
@article{osti_1430531,
title = {Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse},
author = {Liu, Chi and Yao, Minjie and Stegen, James C. and Rui, Junpeng and Li, Jiabao and Li, Xiangzhen},
abstractNote = {How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. Here, we started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightly increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.},
doi = {10.1038/S41598-017-17736-W},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {12}
}

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