Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Singh, Appu K.; Saotome, Kei; Sobolevsky, Alexander I.

doi:10.1038/s41598-017-10993-9

Title: Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Abstract

Tetrameric ion channels have either swapped or non-swapped arrangements of the S1–S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.

Authors:

Singh, Appu K. ^[1]; Saotome, Kei ^[1]; Sobolevsky, Alexander I. ^[1]

Columbia Univ., New York, NY (United States)

Publication Date:: 2017-09-06

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: National Institutes of Health (NIH)

OSTI Identifier:: 1430345

Grant/Contract Number:: R01 NS083660; R01 CA206573; T32 GM008281

Resource Type:: Accepted Manuscript

Journal Name:: Scientific Reports

Additional Journal Information:: Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Ion transport; Nanocrystallography

Citation Formats


                    Singh, Appu K., Saotome, Kei, and Sobolevsky, Alexander I. Swapping of transmembrane domains in the epithelial calcium channel TRPV6.  United States: N. p., 2017. 
Web.  https://doi.org/10.1038/s41598-017-10993-9.

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                    Singh, Appu K., Saotome, Kei, & Sobolevsky, Alexander I. Swapping of transmembrane domains in the epithelial calcium channel TRPV6.  United States.  https://doi.org/10.1038/s41598-017-10993-9

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                    Singh, Appu K., Saotome, Kei, and Sobolevsky, Alexander I. Wed .  
"Swapping of transmembrane domains in the epithelial calcium channel TRPV6".  United States.  https://doi.org/10.1038/s41598-017-10993-9.  https://www.osti.gov/servlets/purl/1430345.

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@article{osti_1430345,

  title        = {Swapping of transmembrane domains in the epithelial calcium channel TRPV6},

  author       = {Singh, Appu K. and Saotome, Kei and Sobolevsky, Alexander I.},

  abstractNote = {Tetrameric ion channels have either swapped or non-swapped arrangements of the S1–S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.},

  doi          = {10.1038/s41598-017-10993-9},

  journal      = {Scientific Reports},

  number       = 1,

  volume       = 7,

  place        = {United States},

  year         = {2017},

  month        = {9}

}

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Title: Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Abstract

Citation Formats

Structures of the Human HCN1 Hyperpolarization-Activated Channel journal, January 2017

Structural Titration of Slo2.2, a Na + -Dependent K + Channel journal, January 2017

Structure of the voltage-gated K+ channel Eag1 reveals an alternative voltage sensing mechanism journal, August 2016

Solving structures of protein complexes by molecular replacement with Phaser journal, December 2006

CaT1 and the Calcium Release-activated Calcium Channel Manifest Distinct Pore Properties journal, October 2001

TRP channels in calcium homeostasis: from hormonal control to structure-function relationship of TRPV5 and TRPV6 journal, June 2017

TRPV6 and prostate cancer: cancer growth beyond the prostate correlates with increased TRPV6 Ca2+ channel expression journal, October 2004

Structure of the TRPV1 ion channel determined by electron cryo-microscopy journal, December 2013

Cryo-electron microscopy structure of the TRPV2 ion channel journal, January 2016

Structure of the full-length TRPV2 channel by cryo-EM journal, March 2016

Structure of a eukaryotic cyclic-nucleotide-gated channel journal, January 2017

Cryo-EM structure of the open high-conductance Ca2+-activated K+ channel journal, December 2016

Erratum: Structure of the TRPA1 ion channel suggests regulatory mechanisms journal, July 2015

Crystal structure of the epithelial calcium channel TRPV6 journal, June 2016

Coot model-building tools for molecular graphics journal, November 2004

The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity journal, April 1998

XDS journal, January 2010

REFMAC 5 for the refinement of macromolecular crystal structures journal, March 2011

[20] Processing of X-ray diffraction data collected in oscillation mode book, January 1997

Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment journal, November 2007

Calcium Channel TRPV6 as a Potential Therapeutic Target in Estrogen Receptor-Negative Breast Cancer journal, July 2012

TRP Channels journal, June 2007

PHENIX: a comprehensive Python-based system for macromolecular structure solution journal, January 2010

Opening of the human epithelial calcium channel TRPV6 journal, December 2017

Structural insights on TRPV5 gating by endogenous modulators journal, October 2018

Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms journal, February 2018

Structure and gating mechanism of the transient receptor potential channel TRPV3 journal, August 2018

X-ray crystallography of TRP channels journal, January 2018

Structural mechanisms of transient receptor potential ion channels journal, January 2020

A hypothetical molecular mechanism for TRPV1 activation that invokes rotation of an S6 asparagine journal, October 2018

Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy: Cryo-EM studies of ion channels journal, March 2018

Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6 journal, August 2018

Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations journal, November 2018

New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question journal, January 2019

Structures of the Human HCN1 Hyperpolarization-Activated Channel
journal, January 2017

Structural Titration of Slo2.2, a Na + -Dependent K + Channel
journal, January 2017

Structure of the voltage-gated K+ channel Eag1 reveals an alternative voltage sensing mechanism
journal, August 2016

Solving structures of protein complexes by molecular replacement with Phaser
journal, December 2006

CaT1 and the Calcium Release-activated Calcium Channel Manifest Distinct Pore Properties
journal, October 2001

TRP channels in calcium homeostasis: from hormonal control to structure-function relationship of TRPV5 and TRPV6
journal, June 2017

TRPV6 and prostate cancer: cancer growth beyond the prostate correlates with increased TRPV6 Ca2+ channel expression
journal, October 2004

Structure of the TRPV1 ion channel determined by electron cryo-microscopy
journal, December 2013

Cryo-electron microscopy structure of the TRPV2 ion channel
journal, January 2016

Structure of the full-length TRPV2 channel by cryo-EM
journal, March 2016

Structure of a eukaryotic cyclic-nucleotide-gated channel
journal, January 2017

Cryo-EM structure of the open high-conductance Ca2+-activated K+ channel
journal, December 2016

Erratum: Structure of the TRPA1 ion channel suggests regulatory mechanisms
journal, July 2015

Crystal structure of the epithelial calcium channel TRPV6
journal, June 2016

Coot model-building tools for molecular graphics
journal, November 2004

The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity
journal, April 1998

XDS
journal, January 2010

REFMAC 5 for the refinement of macromolecular crystal structures
journal, March 2011

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment
journal, November 2007

Calcium Channel TRPV6 as a Potential Therapeutic Target in Estrogen Receptor-Negative Breast Cancer
journal, July 2012

TRP Channels
journal, June 2007

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

Opening of the human epithelial calcium channel TRPV6
journal, December 2017

Structural insights on TRPV5 gating by endogenous modulators
journal, October 2018

Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms
journal, February 2018

Structure and gating mechanism of the transient receptor potential channel TRPV3
journal, August 2018

X-ray crystallography of TRP channels
journal, January 2018

Structural mechanisms of transient receptor potential ion channels
journal, January 2020

A hypothetical molecular mechanism for TRPV1 activation that invokes rotation of an S6 asparagine
journal, October 2018

Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy: Cryo-EM studies of ion channels
journal, March 2018

Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6
journal, August 2018

Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations
journal, November 2018

New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question
journal, January 2019