Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Singh, Appu K.; Saotome, Kei; Sobolevsky, Alexander I.

doi:10.1038/s41598-017-10993-9

Title: Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Journal Article · 05 September 2017 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-10993-9 · OSTI ID:1430345

Singh, Appu K. ^[1]; Saotome, Kei ^[1]; Sobolevsky, Alexander I. ^[1]

Columbia Univ., New York, NY (United States)

Tetrameric ion channels have either swapped or non-swapped arrangements of the S1–S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: NIH

OSTI ID:: 1430345

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Structural Insights on TRPV5 Gating by Endogenous Modulators Hughes, Taylor; Pumroy, Ruth; Yazici, Aysenur Biophysical Journal, Vol. 116, Issue 3 https://doi.org/10.1016/j.bpj.2018.11.2873	journal	February 2019
Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations Fux, Julia E.; Mehta, Amrit; Moffat, Jack Frontiers in Physiology, Vol. 9 https://doi.org/10.3389/fphys.2018.01406	journal	November 2018
TRPV6 as A Target for Cancer Therapy Stewart, John M. Journal of Cancer, Vol. 11, Issue 2 https://doi.org/10.7150/jca.31640	journal	January 2020
Structural insights on TRPV5 gating by endogenous modulators Hughes, Taylor E. T.; Pumroy, Ruth A.; Yazici, Aysenur Torun https://doi.org/10.1101/338798	posted_content	July 2018
Structure and gating mechanism of the transient receptor potential channel TRPV3 Singh, Appu K.; McGoldrick, Luke L.; Sobolevsky, Alexander I. Nature Structural & Molecular Biology, Vol. 25, Issue 9 https://doi.org/10.1038/s41594-018-0108-7	journal	August 2018
Opening of the human epithelial calcium channel TRPV6 McGoldrick, Luke L.; Singh, Appu K.; Saotome, Kei Nature, Vol. 553, Issue 7687 https://doi.org/10.1038/nature25182	journal	December 2017
Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations Fux, Julia E.; Mehta, Amrit; Moffat, Jack Frontiers in Physiology, Vol. 9 https://doi.org/10.3389/fphys.2018.01406	journal	November 2018
A hypothetical molecular mechanism for TRPV1 activation that invokes rotation of an S6 asparagine Kasimova, Marina A.; Yazici, Aysenur Torun; Yudin, Yevgen Journal of General Physiology, Vol. 150, Issue 11 https://doi.org/10.1085/jgp.201812124	journal	October 2018
New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question Barros, Francisco; Pardo, Luis; Domínguez, Pedro International Journal of Molecular Sciences, Vol. 20, Issue 2 https://doi.org/10.3390/ijms20020248	journal	January 2019
X-ray crystallography of TRP channels Singh, Appu K.; McGoldrick, Luke L.; Saotome, Kei Channels, Vol. 12, Issue 1 https://doi.org/10.1080/19336950.2018.1457898	journal	January 2018
New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question Barros, Francisco; Pardo, Luis; Domínguez, Pedro International Journal of Molecular Sciences, Vol. 20, Issue 2 https://doi.org/10.3390/ijms20020248	journal	January 2019
Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6 Singh, Appu K.; McGoldrick, Luke L.; Twomey, Edward C. Science Advances, Vol. 4, Issue 8 https://doi.org/10.1126/sciadv.aau6088	journal	August 2018
Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy: Cryo-EM studies of ion channels Lau, Carus; Hunter, Mark J.; Stewart, Alastair The Journal of Physiology, Vol. 596, Issue 7 https://doi.org/10.1113/jp274888	journal	March 2018
Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6 Singh, Appu K.; McGoldrick, Luke L.; Twomey, Edward C. Science Advances, Vol. 4, Issue 8 https://doi.org/10.1126/sciadv.aau6088	journal	August 2018
Structural mechanisms of transient receptor potential ion channels Cao, Erhu Journal of General Physiology, Vol. 152, Issue 3 https://doi.org/10.1085/jgp.201811998	journal	January 2020
Structure and gating mechanism of the transient receptor potential channel TRPV3 Singh, Appu K.; McGoldrick, Luke L.; Sobolevsky, Alexander I. Nature Structural & Molecular Biology, Vol. 25, Issue 9 https://doi.org/10.1038/s41594-018-0108-7	journal	August 2018
Structural insights on TRPV5 gating by endogenous modulators Hughes, Taylor E. T.; Pumroy, Ruth A.; Yazici, Aysenur Torun https://doi.org/10.1101/338798	posted_content	July 2018
Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms Deng, Zengqin; Paknejad, Navid; Maksaev, Grigory Nature Structural & Molecular Biology, Vol. 25, Issue 3 https://doi.org/10.1038/s41594-018-0037-5	journal	February 2018

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