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Title: Swapping of transmembrane domains in the epithelial calcium channel TRPV6

Abstract

Tetrameric ion channels have either swapped or non-swapped arrangements of the S1–S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.

Authors:
 [1];  [1];  [1]
+ Show Author Affiliations
  1. Columbia Univ., New York, NY (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1430345
Grant/Contract Number:  
R01 NS083660; R01 CA206573; T32 GM008281
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Ion transport; Nanocrystallography

Citation Formats

Singh, Appu K., Saotome, Kei, and Sobolevsky, Alexander I. Swapping of transmembrane domains in the epithelial calcium channel TRPV6. United States: N. p., 2017. Web. doi:10.1038/s41598-017-10993-9.
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Singh, Appu K., Saotome, Kei, & Sobolevsky, Alexander I. Swapping of transmembrane domains in the epithelial calcium channel TRPV6. United States. doi:10.1038/s41598-017-10993-9.
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Singh, Appu K., Saotome, Kei, and Sobolevsky, Alexander I. Wed . "Swapping of transmembrane domains in the epithelial calcium channel TRPV6". United States. doi:10.1038/s41598-017-10993-9. https://www.osti.gov/servlets/purl/1430345.
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@article{osti_1430345,
title = {Swapping of transmembrane domains in the epithelial calcium channel TRPV6},
author = {Singh, Appu K. and Saotome, Kei and Sobolevsky, Alexander I.},
abstractNote = {Tetrameric ion channels have either swapped or non-swapped arrangements of the S1–S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.},
doi = {10.1038/s41598-017-10993-9},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {9}
}
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Journal Article:
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DOI:  10.1038/s41598-017-10993-9
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