Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice
Abstract
Summary Amino acid transporters ( AAT s) play indispensable roles in nutrient allocation during plant development. In this study, we demonstrated that inhibiting expression of the rice amino acid transporter Os AAP 3 increased grain yield due to a formation of larger numbers of tillers as a result of increased bud outgrowth. Elevated expression of Os AAP 3 in transgenic plants resulted in significantly higher amino acid concentrations of Lys, Arg, His, Asp, Ala, Gln, Gly, Thr and Tyr, and inhibited bud outgrowth and rice tillering. However, RNA i of Os AAP 3 decreased significantly Arg, Lys, Asp and Thr concentrations to a small extent, and thus promoted bud outgrowth, increased significantly tiller numbers and effective panicle numbers per plant, and further enhanced significantly grain yield and nitrogen use efficiency ( NUE ). The promoter sequences of Os AAP 3 showed some divergence between Japonica and Indica rice, and expression of the gene was higher in Japonica , which produced fewer tillers than Indica . We generated knockout lines of Os AAP 3 on Japonica ZH 11 and KY 131 using CRISPR technology and found that grain yield could be increased significantly. These results suggest that manipulation of Os AAPmore »
- Authors:
-
[2]
- Center of Applied Biotechnology Wuhan Institute of Bioengineering Wuhan China
- Center of Applied Biotechnology Wuhan Institute of Bioengineering Wuhan China, National Key Laboratory of Crop Genetic Improvement College of Plant Science and Technology Huazhong Agricultural University Wuhan China
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1429543
- Alternate Identifier(s):
- OSTI ID: 1429544
- Resource Type:
- Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 16 Journal Issue: 10; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Lu, Kai, Wu, Bowen, Wang, Jie, Zhu, Wei, Nie, Haipeng, Qian, Junjie, Huang, Weiting, and Fang, Zhongming. Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice. United Kingdom: N. p., 2018.
Web. doi:10.1111/pbi.12907.
Lu, Kai, Wu, Bowen, Wang, Jie, Zhu, Wei, Nie, Haipeng, Qian, Junjie, Huang, Weiting, & Fang, Zhongming. Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice. United Kingdom. https://doi.org/10.1111/pbi.12907
Lu, Kai, Wu, Bowen, Wang, Jie, Zhu, Wei, Nie, Haipeng, Qian, Junjie, Huang, Weiting, and Fang, Zhongming. Sun .
"Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice". United Kingdom. https://doi.org/10.1111/pbi.12907.
@article{osti_1429543,
title = {Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice},
author = {Lu, Kai and Wu, Bowen and Wang, Jie and Zhu, Wei and Nie, Haipeng and Qian, Junjie and Huang, Weiting and Fang, Zhongming},
abstractNote = {Summary Amino acid transporters ( AAT s) play indispensable roles in nutrient allocation during plant development. In this study, we demonstrated that inhibiting expression of the rice amino acid transporter Os AAP 3 increased grain yield due to a formation of larger numbers of tillers as a result of increased bud outgrowth. Elevated expression of Os AAP 3 in transgenic plants resulted in significantly higher amino acid concentrations of Lys, Arg, His, Asp, Ala, Gln, Gly, Thr and Tyr, and inhibited bud outgrowth and rice tillering. However, RNA i of Os AAP 3 decreased significantly Arg, Lys, Asp and Thr concentrations to a small extent, and thus promoted bud outgrowth, increased significantly tiller numbers and effective panicle numbers per plant, and further enhanced significantly grain yield and nitrogen use efficiency ( NUE ). The promoter sequences of Os AAP 3 showed some divergence between Japonica and Indica rice, and expression of the gene was higher in Japonica , which produced fewer tillers than Indica . We generated knockout lines of Os AAP 3 on Japonica ZH 11 and KY 131 using CRISPR technology and found that grain yield could be increased significantly. These results suggest that manipulation of Os AAP 3 expression could be used to increase grain yield in rice.},
doi = {10.1111/pbi.12907},
journal = {Plant Biotechnology Journal},
number = 10,
volume = 16,
place = {United Kingdom},
year = {Sun Mar 25 00:00:00 EDT 2018},
month = {Sun Mar 25 00:00:00 EDT 2018}
}
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