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Title: Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas

Abstract

Harnessing the metabolic potential of uncultured microbial communities is a compelling opportunity for the biotechnology industry, an approach that would vastly expand the portfolio of usable feedstocks. Methane is particularly promising because it is abundant and energy-rich, yet the most efficient methane-activating metabolic pathways involve mixed communities of anaerobic methanotrophic archaea and sulfate reducing bacteria. These communities oxidize methane at high catabolic efficiency and produce chemically reduced by-products at a comparable rate and in near-stoichiometric proportion to methane consumption. These reduced compounds can be used for feedstock and downstream chemical production, and at the production rates observed in situ they are an appealing, cost-effective prospect. Notably, the microbial constituents responsible for this bioconversion are most prominent in select deep-sea sediments, and while they can be kept active at surface pressures, they have not yet been cultured in the lab. In an industrial capacity, deep-sea sediments could be periodically recovered and replenished, but the associated technical challenges and substantial costs make this an untenable approach for full-scale operations. In this study, we present a novel method for incorporating methanotrophic communities into bioindustrial processes through abstraction onto low mass, easily transportable carbon cloth artificial substrates. Using Gulf of Mexico methane seep sedimentmore » as inoculum, optimal physicochemical parameters were established for methane-oxidizing, sulfide-generating mesocosm incubations. Metabolic activity required > ~40% seawater salinity, peaking at 100% salinity and 35 °C. Microbial communities were successfully transferred to a carbon cloth substrate, and rates of methane-dependent sulfide production increased more than threefold per unit volume. Phylogenetic analyses indicated that carbon cloth-based communities were substantially streamlined and were dominated by Desulfotomaculum geothermicum. Fluorescence in situ hybridization microscopy with carbon cloth fibers revealed a novel spatial arrangement of anaerobic methanotrophs and sulfate reducing bacteria suggestive of an electronic coupling enabled by the artificial substrate. This system: 1) enables a more targeted manipulation of methane-activating microbial communities using a low-mass and sediment-free substrate; 2) holds promise for the simultaneous consumption of a strong greenhouse gas and the generation of usable downstream products; and 3) furthers the broader adoption of uncultured, mixed microbial communities for biotechnological use.« less

Authors:
 [1];  [2]; ORCiD logo [1];  [3];  [3];  [4];  [1]
  1. Department of Organismic and Evolutionary Biology, Harvard University, Cambridge Massachusetts
  2. Department of Organismic and Evolutionary Biology, Harvard University, Cambridge Massachusetts, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge Massachusetts
  3. Department of Human Evolutionary Biology, Harvard University, Cambridge Massachusetts
  4. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge Massachusetts
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1429516
Alternate Identifier(s):
OSTI ID: 1434364; OSTI ID: 1557841
Grant/Contract Number:  
AR0000433
Resource Type:
Published Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Name: Biotechnology and Bioengineering Journal Volume: 115 Journal Issue: 6; Journal ID: ISSN 0006-3592
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States
Language:
English
Subject:
03 NATURAL GAS

Citation Formats

Marlow, Jeffrey J., Kumar, Amit, Enalls, Brandon C., Reynard, Linda M., Tuross, Noreen, Stephanopoulos, Gregory, and Girguis, Peter. Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas. United States: N. p., 2018. Web. doi:10.1002/bit.26576.
Marlow, Jeffrey J., Kumar, Amit, Enalls, Brandon C., Reynard, Linda M., Tuross, Noreen, Stephanopoulos, Gregory, & Girguis, Peter. Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas. United States. doi:10.1002/bit.26576.
Marlow, Jeffrey J., Kumar, Amit, Enalls, Brandon C., Reynard, Linda M., Tuross, Noreen, Stephanopoulos, Gregory, and Girguis, Peter. Sat . "Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas". United States. doi:10.1002/bit.26576.
@article{osti_1429516,
title = {Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas},
author = {Marlow, Jeffrey J. and Kumar, Amit and Enalls, Brandon C. and Reynard, Linda M. and Tuross, Noreen and Stephanopoulos, Gregory and Girguis, Peter},
abstractNote = {Harnessing the metabolic potential of uncultured microbial communities is a compelling opportunity for the biotechnology industry, an approach that would vastly expand the portfolio of usable feedstocks. Methane is particularly promising because it is abundant and energy-rich, yet the most efficient methane-activating metabolic pathways involve mixed communities of anaerobic methanotrophic archaea and sulfate reducing bacteria. These communities oxidize methane at high catabolic efficiency and produce chemically reduced by-products at a comparable rate and in near-stoichiometric proportion to methane consumption. These reduced compounds can be used for feedstock and downstream chemical production, and at the production rates observed in situ they are an appealing, cost-effective prospect. Notably, the microbial constituents responsible for this bioconversion are most prominent in select deep-sea sediments, and while they can be kept active at surface pressures, they have not yet been cultured in the lab. In an industrial capacity, deep-sea sediments could be periodically recovered and replenished, but the associated technical challenges and substantial costs make this an untenable approach for full-scale operations. In this study, we present a novel method for incorporating methanotrophic communities into bioindustrial processes through abstraction onto low mass, easily transportable carbon cloth artificial substrates. Using Gulf of Mexico methane seep sediment as inoculum, optimal physicochemical parameters were established for methane-oxidizing, sulfide-generating mesocosm incubations. Metabolic activity required > ~40% seawater salinity, peaking at 100% salinity and 35 °C. Microbial communities were successfully transferred to a carbon cloth substrate, and rates of methane-dependent sulfide production increased more than threefold per unit volume. Phylogenetic analyses indicated that carbon cloth-based communities were substantially streamlined and were dominated by Desulfotomaculum geothermicum. Fluorescence in situ hybridization microscopy with carbon cloth fibers revealed a novel spatial arrangement of anaerobic methanotrophs and sulfate reducing bacteria suggestive of an electronic coupling enabled by the artificial substrate. This system: 1) enables a more targeted manipulation of methane-activating microbial communities using a low-mass and sediment-free substrate; 2) holds promise for the simultaneous consumption of a strong greenhouse gas and the generation of usable downstream products; and 3) furthers the broader adoption of uncultured, mixed microbial communities for biotechnological use.},
doi = {10.1002/bit.26576},
journal = {Biotechnology and Bioengineering},
number = 6,
volume = 115,
place = {United States},
year = {2018},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1002/bit.26576

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Works referenced in this record:

In-silico-driven metabolic engineering of Pseudomonas putida for enhanced production of poly-hydroxyalkanoates
journal, January 2013


Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969


Bacterial Adhesion on Commercially Pure Titanium and Zirconium Oxide Disks: An In Vivo Human Study
journal, February 2004

  • Scarano, Antonio; Piattelli, Maurizio; Caputi, Sergio
  • Journal of Periodontology, Vol. 75, Issue 2
  • DOI: 10.1902/jop.2004.75.2.292

The anaerobic oxidation of methane and sulfate reduction in sediments from Gulf of Mexico cold seeps
journal, May 2004


Industrial biomanufacturing: The future of chemical production
journal, January 2017

  • Clomburg, James M.; Crumbley, Anna M.; Gonzalez, Ramon
  • Science, Vol. 355, Issue 6320
  • DOI: 10.1126/science.aag0804

Evidence for Anaerobic CH 4 Oxidation in Freshwater Peatlands
journal, December 2007


16S ribosomal DNA amplification for phylogenetic study.
journal, January 1991


Microbial synthesis of n-butanol, isobutanol, and other higher alcohols from diverse resources
journal, May 2013


Harnessing the power of microbial autotrophy
journal, September 2016

  • Claassens, Nico J.; Sousa, Diana Z.; dos Santos, Vitor A. P. Martins
  • Nature Reviews Microbiology, Vol. 14, Issue 11
  • DOI: 10.1038/nrmicro.2016.130

Methane Leaks from North American Natural Gas Systems
journal, February 2014


Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria
journal, February 1997


A bridge to nowhere: methane emissions and the greenhouse gas footprint of natural gas
journal, May 2014

  • Howarth, Robert W.
  • Energy Science & Engineering, Vol. 2, Issue 2
  • DOI: 10.1002/ese3.35

Diversity and dynamics of microbial communities in engineered environments and their implications for process stability
journal, June 2003


Bioreactor performance and methanogenic population dynamics in a low-temperature (5–18°C) anaerobic fixed-bed reactor
journal, January 2012


Biogeochemical processes and microbial diversity of the Gullfaks and Tommeliten methane seeps (Northern North Sea)
journal, January 2008

  • Wegener, G.; Shovitri, M.; Knittel, K.
  • Biogeosciences Discussions, Vol. 5, Issue 1
  • DOI: 10.5194/bgd-5-971-2008

Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample
journal, June 2010

  • Caporaso, J. G.; Lauber, C. L.; Walters, W. A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1
  • DOI: 10.1073/pnas.1000080107

Effects of Solution Chemistry on Bacterial Adhesion with Phyllosilicates and Goethite Explained by the Extended DLVO Theory
journal, March 2014


Metabolic engineering in chemolithoautotrophic hosts for the production of fuels and chemicals
journal, July 2015


Natural gas origin, composition, and processing: A review
journal, August 2016


Single cell activity reveals direct electron transfer in methanotrophic consortia
journal, September 2015

  • McGlynn, Shawn E.; Chadwick, Grayson L.; Kempes, Christopher P.
  • Nature, Vol. 526, Issue 7574
  • DOI: 10.1038/nature15512

Embracing the unknown: disentangling the complexities of the soil microbiome
journal, August 2017


Anaerobic oxidation of methane above gas hydrates at Hydrate Ridge, NE Pacific Ocean
journal, January 2003

  • Treude, T.; Boetius, A.; Knittel, K.
  • Marine Ecology Progress Series, Vol. 264
  • DOI: 10.3354/meps264001

Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans
journal, January 2016

  • Nazem-Bokaee, Hadi; Gopalakrishnan, Saratram; Ferry, James G.
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-015-0404-4

Metabolic Capabilities of Microorganisms Involved in and Associated with the Anaerobic Oxidation of Methane
journal, February 2016


Carbonate-hosted methanotrophy represents an unrecognized methane sink in the deep sea
journal, October 2014

  • Marlow, Jeffrey J.; Steele, Joshua A.; Ziebis, Wiebke
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms6094

A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures
journal, August 2000


Prokaryotic Osmoregulation: Genetics and Physiology
journal, October 1991


Desulfotomaculum spp. and related gram-positive sulfate-reducing bacteria in deep subsurface environments
journal, January 2013

  • Aüllo, Thomas; Ranchou-Peyruse, Anthony; Ollivier, Bernard
  • Frontiers in Microbiology, Vol. 4
  • DOI: 10.3389/fmicb.2013.00362

Bacterial community segmentation facilitates the prediction of ecosystem function along the coast of the western Antarctic Peninsula
journal, January 2017

  • Bowman, Jeff S.; Amaral-Zettler, Linda A.; J. Rich, Jeremy
  • The ISME Journal, Vol. 11, Issue 6
  • DOI: 10.1038/ismej.2016.204

Engineering the iron-oxidizing chemolithoautotroph Acidithiobacillus ferrooxidans for biochemical production : Metabolic Engineering of
journal, October 2015

  • Kernan, Timothy; Majumdar, Sudipta; Li, Xiaozheng
  • Biotechnology and Bioengineering, Vol. 113, Issue 1
  • DOI: 10.1002/bit.25703

Temperature dependence and rates of sulfate reduction in cold sediments of svalbard, arctic ocean
journal, April 1998


Growth and Population Dynamics of Anaerobic Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in a Continuous-Flow Bioreactor
journal, July 2005


Microbial eukaryotic distributions and diversity patterns in a deep-sea methane seep ecosystem: Microbial eukaryotic patterns in deep-sea methane seeps
journal, January 2016

  • Pasulka, Alexis L.; Levin, Lisa A.; Steele, Josh A.
  • Environmental Microbiology, Vol. 18, Issue 9
  • DOI: 10.1111/1462-2920.13185

Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico
journal, October 2003


Growth and Methane Oxidation Rates of Anaerobic Methanotrophic Archaea in a Continuous-Flow Bioreactor
journal, September 2003


Tracing the slow growth of anaerobic methane-oxidizing communities by 15N-labelling techniques: 15N-labelling of anaerobic methanotrophs
journal, March 2008


Evaluation and optimization of PCR primers for selective and quantitative detection of marine ANME subclusters involved in sulfate-dependent anaerobic methane oxidation
journal, June 2017

  • Timmers, Peer H. A.; Widjaja-Greefkes, H. C. Aura; Plugge, Caroline M.
  • Applied Microbiology and Biotechnology, Vol. 101, Issue 14
  • DOI: 10.1007/s00253-017-8338-x

Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures
journal, December 2014


Analysis of methane and sulfate flux in methane-charged sediments from the Mississippi Canyon, Gulf of Mexico
journal, November 2008


Thermophilic anaerobic oxidation of methane by marine microbial consortia
journal, June 2011

  • Holler, Thomas; Widdel, Friedrich; Knittel, Katrin
  • The ISME Journal, Vol. 5, Issue 12
  • DOI: 10.1038/ismej.2011.77

Formation processes of framboidal pyrite
journal, January 1997


Autoendoliths: a distinct type of rock-hosted microbial life
journal, April 2015

  • Marlow, J.; Peckmann, J.; Orphan, V.
  • Geobiology, Vol. 13, Issue 4
  • DOI: 10.1111/gbi.12131

Surveys, simulation and single-cell assays relate function and phylogeny in a lake ecosystem
journal, August 2016


Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates
journal, October 2003


Anaerobic Oxidation of Methane in Sediments of Lake Constance, an Oligotrophic Freshwater Lake
journal, May 2011

  • Deutzmann, Jörg S.; Schink, Bernhard
  • Applied and Environmental Microbiology, Vol. 77, Issue 13
  • DOI: 10.1128/AEM.00340-11

Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria
journal, October 2015

  • Wegener, Gunter; Krukenberg, Viola; Riedel, Dietmar
  • Nature, Vol. 526, Issue 7574
  • DOI: 10.1038/nature15733

Reversing methanogenesis to capture methane for liquid biofuel precursors
journal, January 2016

  • Soo, Valerie W. C.; McAnulty, Michael J.; Tripathi, Arti
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-015-0397-z

Spatial Structure and Activity of Sedimentary Microbial Communities Underlying a Beggiatoa spp. Mat in a Gulf of Mexico Hydrocarbon Seep
journal, January 2010


Effective bioremediation of Cadmium (II), nickel (II), and chromium (VI) in a marine environment by using Desulfovibrio desulfuricans
journal, September 2015

  • Joo, Jeong ock; Choi, Jin-Ha; Kim, In Hwa
  • Biotechnology and Bioprocess Engineering, Vol. 20, Issue 5
  • DOI: 10.1007/s12257-015-0287-6

Measurement of Diversity
journal, April 1949


Decoupling of DAMO archaea from DAMO bacteria in a methane-driven microbial fuel cell
journal, March 2017


In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate
journal, January 2007


Filamentous bacteria transport electrons over centimetre distances
journal, October 2012

  • Pfeffer, Christian; Larsen, Steffen; Song, Jie
  • Nature, Vol. 491, Issue 7423
  • DOI: 10.1038/nature11586

Global dispersion and local diversification of the methane seep microbiome
journal, March 2015

  • Ruff, S. Emil; Biddle, Jennifer F.; Teske, Andreas P.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 13
  • DOI: 10.1073/pnas.1421865112

Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction
journal, February 2016


Bioremediation of chromate: thermodynamic analysis of the effects of Cr(VI) on sulfate-reducing bacteria
journal, November 2002

  • B., Chardin; A., Dolla; F., Chaspoul
  • Applied Microbiology and Biotechnology, Vol. 60, Issue 3
  • DOI: 10.1007/s00253-002-1091-8

Microbial abundance and diversity patterns associated with sediments and carbonates from the methane seep environments of Hydrate Ridge, OR
journal, October 2014

  • Marlow, Jeffrey J.; Steele, Joshua A.; Case, David H.
  • Frontiers in Marine Science, Vol. 1
  • DOI: 10.3389/fmars.2014.00044

Effects of Temperature and Pressure on Sulfate Reduction and Anaerobic Oxidation of Methane in Hydrothermal Sediments of Guaymas Basin
journal, February 2004


Correlation of mRNA and protein in complex biological samples
journal, October 2009


Theoretical analysis of natural gas recovery from marginal wells with a deep well reactor
journal, April 2017

  • Emerson, David Frederic; Al Ghatta, Amir; Woolston, Benjamin M.
  • AIChE Journal, Vol. 63, Issue 9
  • DOI: 10.1002/aic.15738

Oxidation of hydrogen sulfide byThiobacillus denitrificans: Desulfurization of natural gas
journal, February 1987

  • Sublette, Kerry L.; Sylvester, Nicholas D.
  • Biotechnology and Bioengineering, Vol. 29, Issue 2
  • DOI: 10.1002/bit.260290216

Diverse sulfate-reducing bacteria of the Desulfosarcina/Desulfococcus clade are the key alkane degraders at marine seeps
journal, April 2014

  • Kleindienst, Sara; Herbst, Florian-Alexander; Stagars, Marion
  • The ISME Journal, Vol. 8, Issue 10
  • DOI: 10.1038/ismej.2014.51

Search and clustering orders of magnitude faster than BLAST
journal, August 2010


Unexplored Archaeal Diversity in the Great Ape Gut Microbiome
journal, February 2017


Polyhydroxyalkanoates, challenges and opportunities
journal, December 2014


UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013


Long-term performance of sediment microbial fuel cells with multiple anodes
journal, August 2017


Bacterial synthesis of biodegradable polyhydroxyalkanoates
journal, June 2007


Methane Seep Carbonates Host Distinct, Diverse, and Dynamic Microbial Assemblages
journal, December 2015

  • Case, David H.; Pasulka, Alexis L.; Marlow, Jeffrey J.
  • mBio, Vol. 6, Issue 6
  • DOI: 10.1128/mBio.01348-15

Desulfotomaculum geothermicum sp. nov., a thermophilic, fatty acid-degrading, sulfate-reducing bacterium isolated with H2 from geothermal ground water
journal, January 1988

  • Daumas, S.; Cord-Ruwisch, R.; Garcia, J. L.
  • Antonie van Leeuwenhoek, Vol. 54, Issue 2
  • DOI: 10.1007/BF00419203

Rethinking biological activation of methane and conversion to liquid fuels
journal, April 2014


Methane activation: the past and future
journal, January 2014

  • Tang, Pei; Zhu, Qingjun; Wu, Zhaoxuan
  • Energy Environ. Sci., Vol. 7, Issue 8
  • DOI: 10.1039/C4EE00604F

Consumption of Methane and CO2 by Methanotrophic Microbial Mats from Gas Seeps of the Anoxic Black Sea
journal, February 2007

  • Treude, T.; Orphan, V.; Knittel, K.
  • Applied and Environmental Microbiology, Vol. 73, Issue 7
  • DOI: 10.1128/AEM.02685-06

Thermophilic archaea activate butane via alkyl-coenzyme M formation
journal, October 2016

  • Laso-Pérez, Rafael; Wegener, Gunter; Knittel, Katrin
  • Nature, Vol. 539, Issue 7629
  • DOI: 10.1038/nature20152

A marine microbial consortium apparently mediating anaerobic oxidation of methane
journal, October 2000

  • Boetius, Antje; Ravenschlag, Katrin; Schubert, Carsten J.
  • Nature, Vol. 407, Issue 6804
  • DOI: 10.1038/35036572

Characterization of endogenous promoters for control of recombinant gene expression in Acidithiobacillus ferrooxidans : Endogenous Promoters for Use in
journal, May 2017

  • Kernan, Timothy; West, Alan C.; Banta, Scott
  • Biotechnology and Applied Biochemistry, Vol. 64, Issue 6
  • DOI: 10.1002/bab.1546

Bacterial diversity promotes community stability and functional resilience after perturbation
journal, March 2005


Anaerobic oxidation of methane associated with sulfate reduction in a natural freshwater gas source
journal, December 2015

  • Timmers, Peer HA; Suarez-Zuluaga, Diego A.; van Rossem, Minke
  • The ISME Journal, Vol. 10, Issue 6
  • DOI: 10.1038/ismej.2015.213

Bioremediation of an industrial acid mine water by metal-tolerant sulphate-reducing bacteria
journal, September 2001


Deep-Sea Archaea Fix and Share Nitrogen in Methane-Consuming Microbial Consortia
journal, October 2009


The ecology and biotechnology of sulphate-reducing bacteria
journal, May 2008

  • Muyzer, Gerard; Stams, Alfons J. M.
  • Nature Reviews Microbiology, Vol. 6, Issue 6
  • DOI: 10.1038/nrmicro1892

Organic loading rate shock impact on operation and microbial communities in different anaerobic fixed-bed reactors
journal, July 2013


Temperature dependence of metabolic rates for microbial growth, maintenance, and survival
journal, March 2004

  • Price, P. B.; Sowers, T.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 13
  • DOI: 10.1073/pnas.0400522101

Metabolic engineering of Methanosarcina acetivorans for lactate production from methane : Anaerobic Lactate Production From Methane
journal, November 2016

  • McAnulty, Michael J.; Poosarla, Venkata Giridhar; Li, Jine
  • Biotechnology and Bioengineering, Vol. 114, Issue 4
  • DOI: 10.1002/bit.26208

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012

  • Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
  • The ISME Journal, Vol. 6, Issue 8
  • DOI: 10.1038/ismej.2012.8

Methane-derived carbonates and authigenic pyrite from the northwestern Black Sea
journal, June 2001


Aerobic and anaerobic metabolism of squalene by a denitrifying bacterium isolated from marine sediment
journal, October 2002

  • Rontani, Jean-François; Mouzdahir, Abdelkrim; Michotey, Valérie
  • Archives of Microbiology, Vol. 178, Issue 4
  • DOI: 10.1007/s00203-002-0457-8

A35 Molecular epidemiology of respiratory viruses
journal, April 2018


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