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Title: Metastable Prepores in Tension-Free Lipid Bilayers

Abstract

The formation and closure of aqueous pores in lipid bilayers is a key step in various biophysical processes. Large pores are well described by classical nucleation theory, but the free-energy landscape of small, biologically relevant pores has remained largely unexplored. The existence of small and metastable “prepores” was hypothesized decades ago from electroporation experiments, but resolving metastable prepores from theoretical models remained challenging. Using two complementary methods—atomistic simulations and self-consistent field theory of a minimal lipid model—we determine the parameters for which metastable prepores occur in lipid membranes. Here, both methods consistently suggest that pore metastability depends on the relative volume ratio between the lipid head group and lipid tails: lipids with a larger head-group volume fraction (or shorter saturated tails) form metastable prepores, whereas lipids with a smaller head-group volume fraction (or longer unsaturated tails) form unstable prepores.

Authors:
 [1];  [2];  [3];  [2]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  2. Univ. of Goettingen (Germany). Inst. for Microbiology and Genetics; Gottingen Center for Molecular Biosciences, Gottingen (Germany)
  3. Univ. of Goettingen (Germany). Inst. for Theoretical Physics
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1429498
Alternate Identifier(s):
OSTI ID: 1429141
Report Number(s):
SAND-2018-1181J
Journal ID: ISSN 0031-9007; PRLTAO; 660445; TRN: US1802477
Grant/Contract Number:  
AC04-94AL85000; NA0003525
Resource Type:
Accepted Manuscript
Journal Name:
Physical Review Letters
Additional Journal Information:
Journal Volume: 120; Journal Issue: 12; Journal ID: ISSN 0031-9007
Publisher:
American Physical Society (APS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ting, Christina L., Awasthi, Neha, Muller, Marcus, and Hub, Jochen S. Metastable Prepores in Tension-Free Lipid Bilayers. United States: N. p., 2018. Web. doi:10.1103/PhysRevLett.120.128103.
Ting, Christina L., Awasthi, Neha, Muller, Marcus, & Hub, Jochen S. Metastable Prepores in Tension-Free Lipid Bilayers. United States. doi:10.1103/PhysRevLett.120.128103.
Ting, Christina L., Awasthi, Neha, Muller, Marcus, and Hub, Jochen S. Fri . "Metastable Prepores in Tension-Free Lipid Bilayers". United States. doi:10.1103/PhysRevLett.120.128103. https://www.osti.gov/servlets/purl/1429498.
@article{osti_1429498,
title = {Metastable Prepores in Tension-Free Lipid Bilayers},
author = {Ting, Christina L. and Awasthi, Neha and Muller, Marcus and Hub, Jochen S.},
abstractNote = {The formation and closure of aqueous pores in lipid bilayers is a key step in various biophysical processes. Large pores are well described by classical nucleation theory, but the free-energy landscape of small, biologically relevant pores has remained largely unexplored. The existence of small and metastable “prepores” was hypothesized decades ago from electroporation experiments, but resolving metastable prepores from theoretical models remained challenging. Using two complementary methods—atomistic simulations and self-consistent field theory of a minimal lipid model—we determine the parameters for which metastable prepores occur in lipid membranes. Here, both methods consistently suggest that pore metastability depends on the relative volume ratio between the lipid head group and lipid tails: lipids with a larger head-group volume fraction (or shorter saturated tails) form metastable prepores, whereas lipids with a smaller head-group volume fraction (or longer unsaturated tails) form unstable prepores.},
doi = {10.1103/PhysRevLett.120.128103},
journal = {Physical Review Letters},
number = 12,
volume = 120,
place = {United States},
year = {2018},
month = {3}
}

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