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Title: Molecular cloning of the tomato Hairless gene implicates actin dynamics in trichome-mediated defense and mechanical properties of stem tissue

Trichomes are epidermal structures that provide a first line of defense against arthropod herbivores. The recessive hairless (hl) mutation in tomato (Solanum lycopersicum L.) causes severe distortion of trichomes on all aerial tissues, impairs the accumulation of sesquiterpene and polyphenolic compounds in glandular trichomes, and compromises resistance to the specialist herbivore Manduca sexta. Here, we demonstrate that the tomato Hl gene encodes a subunit (SRA1) of the highly conserved WAVE regulatory complex that controls nucleation of actin filaments in a wide range of eukaryotic cells. The tomato SRA1 gene spans a 42-kb region containing both Solyc11g013280 and Solyc11g013290. The hl mutation corresponds to a complex 3-kb deletion that removes the last exon of the gene. Expression of a wild-type SRA1 cDNA in the hl mutant background restored normal trichome development, accumulation of glandular trichomederived metabolites, and resistance to insect herbivory. These findings thus establish a role for SRA1 in the development of tomato trichomes and also implicate the actin-cytoskeleton network in cytosolic control of specialized metabolism for plant defense. We also show that the brittleness of hl mutant stems is associated with altered mechanical and cell morphological properties of stem tissue, and demonstrate that this defect is directly linked tomore » the mutation in SRA1.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [4] ;  [3] ; ORCiD logo [6]
  1. Michigan State Univ., East Lansing, MI (United States). Dept. of Energy-Plant Research Lab.; Seoul National Univ. (Korea, Republic of). Graduate School of International Agricultural Technology and Crop Biotechnology Inst. and GreenBio Science and Technology
  2. Michigan State Univ., East Lansing, MI (United States). Dept. of Energy-Plant Research Lab.; Univ. of Brasillia (Brazil). Inst. of Biological Sciences and Dept. of Botany
  3. Michigan State Univ., East Lansing, MI (United States). Dept. of Energy-Plant Research Lab. and Dept. of Plant Biology
  4. Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology
  5. Michigan State Univ., East Lansing, MI (United States). Dept. of Chemistry and Dept. of Biochemistry and Molecular Biology
  6. Michigan State Univ., East Lansing, MI (United States). Dept. of Energy-Plant Research Lab. and Dept. of Biochemistry and Molecular Biology
Publication Date:
Grant/Contract Number:
FC02-07ER64494; FG02-91ER20021; DBI-0604336; IOS-1456864
Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 67; Journal Issue: 18; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Research Org:
Michigan State Univ., East Lansing, MI (United States); Univ. of California, Davis, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF); Organisation for Economic Co-operation and Development (OECD); Rural Development Administration (Korea, Republic of)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Actin-cytoskeleton network; cell wall; mechanical properties; plant-insect interaction; specialized metabolism; terpenoid; tomato (Solanum lycopersicum); trichome
OSTI Identifier:
1427733