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Title: FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells

Abstract

Triacylglycerol (TAG) is the main storage lipid in plant seeds and the major form of plant oil used for food and, increasingly, for industrial and biofuel applications. Several transcription factors, including FUSCA3 (At3 g26790, FUS3), are associated with embryo maturation and oil biosynthesis in seeds. However, the ability of FUS3 to increase TAG biosynthesis in other tissues has not been quantitatively examined. Here, we evaluated the ability of FUS3 to activate TAG accumulation in non-seed tissues. Overexpression of FUS3 driven by an estradiol-inducible promoter increased oil contents in Arabidopsis seedlings up to 6% of dry weight; more than 50-fold over controls. Eicosenoic acid, a characteristic fatty acid of Arabidopsis seed oil, accumulated to over 20% of fatty acids in cotyledons and leaves. These large increases depended on added sucrose, although without sucrose TAG increased three- to four-fold. Inducing the expression of FUS3 in tobacco BY2 cells also increased TAG accumulation, and co-expression of FUS3 and diacylglycerol acyltransferase 1 (DGAT1) further increased TAG levels to 4% of dry weight. BY2 cell growth was not altered by FUS3 expression, although Arabidopsis seedling development was impaired, consistent with the ability of FUS3 to induce embryo characteristics in non-seed tissues. Microarrays of Arabidopsis seedlingsmore » revealed that FUS3 overexpression increased the expression of a higher proportion of genes involved in TAG biosynthesis than genes involved in fatty acid biosynthesis or other lipid pathways. Together these results provide additional insights into FUS3 functions in TAG metabolism and suggest complementary strategies for engineering vegetative oil accumulation.« less

Authors:
 [1];  [2];  [3];  [2]
  1. Northwest A&F University, Yangling, Shaanxi (China). College of Agronomy; Michigan State Univ., East Lansing, MI (United States). Department of Plant Biology and Great Lakes Bioenergy Research Center
  2. Michigan State Univ., East Lansing, MI (United States). Department of Plant Biology and Great Lakes Bioenergy Research Center
  3. Northwest A&F University, Yangling, Shaanxi (China). College of Agronomy
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1427683
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 88; Journal Issue: 1; Journal ID: ISSN 0960-7412
Publisher:
Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; FUSCA3; triacylglycerol; vegetative tissue; microarray; arabidopsis; tobacco

Citation Formats

Zhang, Meng, Cao, Xia, Jia, Qingli, and Ohlrogge, John. FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells. United States: N. p., 2016. Web. doi:10.1111/tpj.13233.
Zhang, Meng, Cao, Xia, Jia, Qingli, & Ohlrogge, John. FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells. United States. doi:10.1111/tpj.13233.
Zhang, Meng, Cao, Xia, Jia, Qingli, and Ohlrogge, John. Sat . "FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells". United States. doi:10.1111/tpj.13233. https://www.osti.gov/servlets/purl/1427683.
@article{osti_1427683,
title = {FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells},
author = {Zhang, Meng and Cao, Xia and Jia, Qingli and Ohlrogge, John},
abstractNote = {Triacylglycerol (TAG) is the main storage lipid in plant seeds and the major form of plant oil used for food and, increasingly, for industrial and biofuel applications. Several transcription factors, including FUSCA3 (At3 g26790, FUS3), are associated with embryo maturation and oil biosynthesis in seeds. However, the ability of FUS3 to increase TAG biosynthesis in other tissues has not been quantitatively examined. Here, we evaluated the ability of FUS3 to activate TAG accumulation in non-seed tissues. Overexpression of FUS3 driven by an estradiol-inducible promoter increased oil contents in Arabidopsis seedlings up to 6% of dry weight; more than 50-fold over controls. Eicosenoic acid, a characteristic fatty acid of Arabidopsis seed oil, accumulated to over 20% of fatty acids in cotyledons and leaves. These large increases depended on added sucrose, although without sucrose TAG increased three- to four-fold. Inducing the expression of FUS3 in tobacco BY2 cells also increased TAG accumulation, and co-expression of FUS3 and diacylglycerol acyltransferase 1 (DGAT1) further increased TAG levels to 4% of dry weight. BY2 cell growth was not altered by FUS3 expression, although Arabidopsis seedling development was impaired, consistent with the ability of FUS3 to induce embryo characteristics in non-seed tissues. Microarrays of Arabidopsis seedlings revealed that FUS3 overexpression increased the expression of a higher proportion of genes involved in TAG biosynthesis than genes involved in fatty acid biosynthesis or other lipid pathways. Together these results provide additional insights into FUS3 functions in TAG metabolism and suggest complementary strategies for engineering vegetative oil accumulation.},
doi = {10.1111/tpj.13233},
journal = {The Plant Journal},
number = 1,
volume = 88,
place = {United States},
year = {2016},
month = {6}
}

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Works referenced in this record:

Plant triacylglycerols as feedstocks for the production of biofuels
journal, May 2008


Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
journal, May 2000

  • Dahlqvist, A.; Stahl, U.; Lenman, M.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6487-6492
  • DOI: 10.1073/pnas.120067297

Tobacco as a production platform for biofuel overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass
journal, April 2010