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Title: Comparative estrogenicity of endogenous, environmental and dietary estrogens in pregnant women I: Serum levels, variability and the basis for urinary biomonitoring of serum estrogenicity

Biomonitoring of human exposure to estrogens most frequently focuses on environmental and dietary estrogens, and infrequently includes measures of exposure to potent endogenous estrogens present in serum. Pregnancy is a developmentally sensitive period during which “added” serum estrogenicity exceeding normal intra-individual daily variability may be of particular relevance. Here, we made repeated measurements of serum concentrations of estrone (E1), estradiol (E2), estriol (E3), estetrol (E4), daidzein (DDZ), genistein (GEN) and bisphenol A (BPA) in thirty pregnant women using ultra-performance liquid chromatography coupled with tandem mass spectrometry detection (UPLC-MS/MS) and electrospray ionization (ESI). Serum E1, E2, and E3 concentrations varied significantly (coefficients of variation 9–10%) with broad ranges across the cohort: 1.61–85.1 nM, 9.09–69.7 nM, and 1.5–36.3 nM respectively. BPA (undetected, estimated from total exposure), DDZ and GEN concentrations were 1-5 orders of magnitude lower. The 24-h urinary elimination profiles of endogenous estrogens were each strongly correlated with their corresponding serum concentrations (Pearson's Correlation Coefficients of 0.83 (E1), 0.84 (E2) and 0.94 (E3)). Lastly, a multivariate regression analysis produced equations for estimating serum concentrations of E1, E2, E3, E4, GEN and DDZ from urinary elimination rates and gestation period, an important step towards non-invasive biomonitoring for assessment of “added” estrogenicity duringmore » pregnancy.« less
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  1. US Food and Drug Administration, Jefferson, AR (United States). National Center for Toxicological Research, Division of Biochemical Toxicology
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Health Effects and Exposure Science
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Health Effects and Exposure Science; Oregon State Univ., Corvallis, OR (United States). Dept. of Environmental and Molecular Toxicology
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Food and Chemical Toxicology
Additional Journal Information:
Journal Volume: 115; Journal ID: ISSN 0278-6915
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE; USFDA; German Academic Exchange Service (DAAD)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; Bisphenol A; Estrogens; Pharmacokinetics; Exposure; Pregnancy; Biomonitoring; Endocrine disruptors
OSTI Identifier: