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Title: Comparative energetics of carbon storage molecules in green algae

Abstract

Several members of the green algae possess the ability to produce lipids and/or high value compounds in significant quantities. While for several of these green algal species induction of increased lipid production has been shown, and cultivation of species for high value molecules occurs at production scale, the molecular mechanisms governing over-accumulation of molecules synthesized from isoprenoid precursors, carotenoids, for example, have received far less attention. Here, we present a calculation of the required ATP equivalencies per carbon atom and reducing power equivalencies as NADH/NADPH (NAD(P)H) per carbon atom for the isoprenoid molecules ..beta..-carotene (C40), astaxanthin (C40), and squalene (C30). We compared energetic requirements of carbohydrates, triacylglycerol, and isoprenoid molecules under a gradient of conditions of cellular stress. Our calculations revealed slightly less ATP and NAD(P)H equivalency per carbon atom between triacylglycerol and the three isoprenoid molecules. Based on our results, we propose that the driving force for differences in accumulation patterns of carotenoids vs. triacylglycerols in algal cells under stress is largely dependent on the presence and regulation of bypass mechanisms at metabolic junction bottlenecks, like pyruvate dehydrogenase (PDH), within particular species. We provide a discussion of several molecular mechanisms that may influence carbon partitioning within different groups ofmore » green algae, including metabolic inhibition through accumulation of specific substrates related to ATP and reducing equivalent production (NAD(P)H) as well as cellular compartmentalization. This work contributes to the ongoing discussion of cellular homeostatic regulation during stress, as well as the potential mechanisms driving long-term carbon storage as it relates to energy and redox states within the algal cell.« less

Authors:
 [1];  [2];  [1];  [1]
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  1. City Univ. (CUNY), NY (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1427343
Alternate Identifier(s):
OSTI ID: 1694265
Report Number(s):
NREL/JA-5100-71139
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
AC36-08GO28308; SC0012556
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 31; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; green algae; energetics; lipid production

Citation Formats

McKie-Krisberg, Zaid M., Laurens, Lieve M. L., Huang, Andy, and Polle, Jurgen E. W. Comparative energetics of carbon storage molecules in green algae. United States: N. p., 2018. Web. doi:10.1016/j.algal.2018.01.018.
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McKie-Krisberg, Zaid M., Laurens, Lieve M. L., Huang, Andy, & Polle, Jurgen E. W. Comparative energetics of carbon storage molecules in green algae. United States. https://doi.org/10.1016/j.algal.2018.01.018
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McKie-Krisberg, Zaid M., Laurens, Lieve M. L., Huang, Andy, and Polle, Jurgen E. W. Wed . "Comparative energetics of carbon storage molecules in green algae". United States. https://doi.org/10.1016/j.algal.2018.01.018. https://www.osti.gov/servlets/purl/1427343.
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@article{osti_1427343,
title = {Comparative energetics of carbon storage molecules in green algae},
author = {McKie-Krisberg, Zaid M. and Laurens, Lieve M. L. and Huang, Andy and Polle, Jurgen E. W.},
abstractNote = {Several members of the green algae possess the ability to produce lipids and/or high value compounds in significant quantities. While for several of these green algal species induction of increased lipid production has been shown, and cultivation of species for high value molecules occurs at production scale, the molecular mechanisms governing over-accumulation of molecules synthesized from isoprenoid precursors, carotenoids, for example, have received far less attention. Here, we present a calculation of the required ATP equivalencies per carbon atom and reducing power equivalencies as NADH/NADPH (NAD(P)H) per carbon atom for the isoprenoid molecules ..beta..-carotene (C40), astaxanthin (C40), and squalene (C30). We compared energetic requirements of carbohydrates, triacylglycerol, and isoprenoid molecules under a gradient of conditions of cellular stress. Our calculations revealed slightly less ATP and NAD(P)H equivalency per carbon atom between triacylglycerol and the three isoprenoid molecules. Based on our results, we propose that the driving force for differences in accumulation patterns of carotenoids vs. triacylglycerols in algal cells under stress is largely dependent on the presence and regulation of bypass mechanisms at metabolic junction bottlenecks, like pyruvate dehydrogenase (PDH), within particular species. We provide a discussion of several molecular mechanisms that may influence carbon partitioning within different groups of green algae, including metabolic inhibition through accumulation of specific substrates related to ATP and reducing equivalent production (NAD(P)H) as well as cellular compartmentalization. This work contributes to the ongoing discussion of cellular homeostatic regulation during stress, as well as the potential mechanisms driving long-term carbon storage as it relates to energy and redox states within the algal cell.},
doi = {10.1016/j.algal.2018.01.018},
journal = {Algal Research},
number = C,
volume = 31,
place = {United States},
year = {2018},
month = {2}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1016/j.algal.2018.01.018
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Cited by: 4 works
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Figures / Tables:

Figure 1 Figure 1: Shown is an image of an orange cell of D. salina. The orange colour indicates that the cell has over-accumulated β-carotene. The cell has two fagella demonstrating that it is still motile under the carotenogenic conditions.
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Works referenced in this record:

Occurrence, biosynthesis and function of isoprenoid quinones
journal, September 2010

Nutritionally important carotenoids as consumer products
journal, July 2014

  • Berman, Judit; Zorrilla-López, Uxue; Farré, Gemma
  • Phytochemistry Reviews, Vol. 14, Issue 5
  • DOI: 10.1007/s11101-014-9373-1

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
journal, May 2008

Life Cycle GHG Emissions from Microalgal Biodiesel – A CA-GREET Model
journal, May 2014

  • Woertz, Ian C.; Benemann, John R.; Du, Niu
  • Environmental Science & Technology, Vol. 48, Issue 11
  • DOI: 10.1021/es403768q

Microalgae for the production of bulk chemicals and biofuels
journal, May 2010

  • Wijffels, Rene H.; Barbosa, Maria J.; Eppink, Michel H. M.
  • Biofuels, Bioproducts and Biorefining, Vol. 4, Issue 3
  • DOI: 10.1002/bbb.215

Development of western biotechnology's algal β-carotene plant
journal, January 1991

Chlorella for protein and biofuels: from strain selection to outdoor cultivation in a Green Wall Panel photobioreactor
journal, January 2014

  • Guccione, Alessia; Biondi, Natascia; Sampietro, Giacomo
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-84

Commercial applications of microalgae
journal, February 2006

  • Spolaore, Pauline; Joannis-Cassan, Claire; Duran, Elie
  • Journal of Bioscience and Bioengineering, Vol. 101, Issue 2
  • DOI: 10.1263/jbb.101.87

Outdoor cultivation of microalgae for carotenoid production: current state and perspectives
journal, February 2007

  • Del Campo, José A.; García-González, Mercedes; Guerrero, Miguel G.
  • Applied Microbiology and Biotechnology, Vol. 74, Issue 6
  • DOI: 10.1007/s00253-007-0844-9

Review of the cultivation program within the National Alliance for Advanced Biofuels and Bioproducts
journal, March 2017

Phylogenetic Diversity in the Macromolecular Composition of Microalgae
journal, May 2016

Carbon partitioning in photosynthesis
journal, June 2013

Evaluation of novel starch-deficient mutants of Chlorella sorokiniana for hyper-accumulation of lipids
journal, November 2015

Perspectives on engineering strategies for improving biofuel production from microalgae — A critical review
journal, December 2014

Metabolite Profiling and Integrative Modeling Reveal Metabolic Constraints for Carbon Partitioning under Nitrogen Starvation in the Green Algae Haematococcus pluvialis
journal, September 2014

  • Recht, Lee; Töpfer, Nadine; Batushansky, Albert
  • Journal of Biological Chemistry, Vol. 289, Issue 44
  • DOI: 10.1074/jbc.M114.555144

Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana
journal, March 2015

Comparative energetics and kinetics of autotrophic lipid and starch metabolism in chlorophytic microalgae: implications for biomass and biofuel production
journal, January 2013

  • Subramanian, Sowmya; Barry, Amanda N.; Pieris, Shayani
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-150

TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation
journal, June 2012

Lipids and lipid metabolism in eukaryotic algae
journal, March 2006

Lipid metabolism in microalgae distinguishes itself
journal, April 2013

Lipid Droplets and Cellular Lipid Metabolism
journal, July 2012

Enhanced acetyl-CoA production is associated with increased triglyceride accumulation in the green alga Chlorella desiccata
journal, April 2015

  • Avidan, Omri; Brandis, Alexander; Rogachev, Ilana
  • Journal of Experimental Botany, Vol. 66, Issue 13
  • DOI: 10.1093/jxb/erv166

Downregulation of a putative plastid PDC E1α subunit impairs photosynthetic activity and triacylglycerol accumulation in nitrogen-starved photoautotrophic Chlamydomonas reinhardtii
journal, September 2014

  • Shtaida, Nastassia; Khozin-Goldberg, Inna; Solovchenko, Alexei
  • Journal of Experimental Botany, Vol. 65, Issue 22
  • DOI: 10.1093/jxb/eru374

Some kinetic and regulatory properties of the pea chloroplast pyruvate dehydrogenase complex
journal, April 1988

  • Camp, Pamela J.; Miernyk, Jan A.; Randall, Douglas D.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 933, Issue 2
  • DOI: 10.1016/0005-2728(88)90034-5

Microalgae as Sources of Carotenoids
journal, April 2011

  • Guedes, Ana Catarina; Amaro, Helena M.; Malcata, Francisco Xavier
  • Marine Drugs, Vol. 9, Issue 4
  • DOI: 10.3390/md9040625

Microalgal biotechnology: Carotenoid production by the green algaeDunaliella salina
journal, December 2003

  • Jin, Eon Seon; Melis, Anastasios
  • Biotechnology and Bioprocess Engineering, Vol. 8, Issue 6
  • DOI: 10.1007/BF02949276

Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress
journal, August 2010

Isoprenoid biosynthesis in eukaryotic phototrophs: A spotlight on algae
journal, April 2012

Botryococcus braunii: a rich source for hydrocarbons and related ether lipids
journal, December 2004

Colony Organization in the Green Alga Botryococcus braunii (Race B) Is Specified by a Complex Extracellular Matrix
journal, August 2012

  • Weiss, Taylor L.; Roth, Robyn; Goodson, Carrie
  • Eukaryotic Cell, Vol. 11, Issue 12
  • DOI: 10.1128/EC.00184-12

The 1-Deoxy-D-Xylulose-5-Phosphate Pathway of Isoprenoid Biosynthesis in Plants
journal, June 1999

Dynamic flexibility in the light reactions of photosynthesis governed by both electron and proton transfer reactions
journal, July 2004

Enhanced Photosynthesis and Redox Energy Production Contribute to Salinity Tolerance in Dunaliella as Revealed by Homology-Based Proteomics
journal, August 2004

  • Liska, Adam J.; Shevchenko, Andrej; Pick, Uri
  • Plant Physiology, Vol. 136, Issue 1
  • DOI: 10.1104/pp.104.039438

Measurement of Photorespiration in Algae
journal, January 1982

  • Birmingham, Brendan C.; Coleman, John R.; Colman, Brian
  • Plant Physiology, Vol. 69, Issue 1
  • DOI: 10.1104/pp.69.1.259

Photosynthesis and Photorespiration in Algae
journal, May 1977

  • Lloyd, Nigel D. H.; Canvin, David T.; Culver, David A.
  • Plant Physiology, Vol. 59, Issue 5
  • DOI: 10.1104/pp.59.5.936

Cyclic, pseudocyclic and noncyclic photophosphorylation: new links in the chain
journal, January 2003

Regulation of electron transport in microalgae
journal, August 2011

  • Cardol, Pierre; Forti, Giorgio; Finazzi, Giovanni
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 8
  • DOI: 10.1016/j.bbabio.2010.12.004

Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition
journal, May 2014

  • Laurens, Lieve M. L.; Van Wychen, Stefanie; McAllister, Jordan P.
  • Analytical Biochemistry, Vol. 452
  • DOI: 10.1016/j.ab.2014.02.009

Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification
journal, February 2012

  • Laurens, Lieve M. L.; Quinn, Matthew; Van Wychen, Stefanie
  • Analytical and Bioanalytical Chemistry, Vol. 403, Issue 1
  • DOI: 10.1007/s00216-012-5814-0

Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga
journal, November 2012

Fatty acid composition and physiological properties of some filamentous blue-green algae
journal, January 1972

  • Kenyon, C. N.; Rippka, R.; Stanier, R. Y.
  • Archiv f�r Mikrobiologie, Vol. 83, Issue 3
  • DOI: 10.1007/BF00645123

Analysis of Triacylglycerols and Free Fatty Acids in Algae Using Ultra-Performance Liquid Chromatography Mass Spectrometry
journal, September 2012

  • Samburova, Vera; Lemos, Mark S.; Hiibel, Sage
  • Journal of the American Oil Chemists' Society, Vol. 90, Issue 1
  • DOI: 10.1007/s11746-012-2138-3

Effects of Growth Irradiance and Nitrogen Limitation on Photosynthetic Energy Conversion in Photosystem II
journal, November 1988

  • Kolber, Zbigniew; Zehr, Jonathan; Falkowski, Paul
  • Plant Physiology, Vol. 88, Issue 3
  • DOI: 10.1104/pp.88.3.923

Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis in Haematococcus pluvialis (Chlorophyceae)
journal, November 2014

  • Chen, Guanqun; Wang, Baobei; Han, Danxiang
  • The Plant Journal, Vol. 81, Issue 1
  • DOI: 10.1111/tpj.12713

The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features
journal, April 2011

Draft Nuclear Genome Sequence of the Halophilic and Beta-Carotene-Accumulating Green Alga Dunaliella salina Strain CCAP19/18
journal, October 2017

  • Polle, Juergen E. W.; Barry, Kerrie; Cushman, John
  • Genome Announcements, Vol. 5, Issue 43
  • DOI: 10.1128/genomeA.01105-17

Acetyl-CoA synthetase is activated as part of the PDH-bypass in the oleaginous green alga Chlorella desiccata
journal, September 2015

  • Avidan, Omri; Pick, Uri
  • Journal of Experimental Botany, Vol. 66, Issue 22
  • DOI: 10.1093/jxb/erv424

Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways
journal, November 2009

  • Joyard, Jacques; Ferro, Myriam; Masselon, Christophe
  • Molecular Plant, Vol. 2, Issue 6
  • DOI: 10.1093/mp/ssp088

Induced β-Carotene Synthesis Driven by Triacylglycerol Deposition in the Unicellular Alga Dunaliella bardawil
journal, April 1998

  • Rabbani, Said; Beyer, Peter; Lintig, Johannes v.
  • Plant Physiology, Vol. 116, Issue 4
  • DOI: 10.1104/pp.116.4.1239

Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina
journal, June 2009

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    Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina
    journal, January 2020

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