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Title: Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

Abstract

Water-born pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. We present an innovative centrifugal microfluidic platform (SpinDx) for detection of bacterial pathogens using bead-based immunoassays. Our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a density-media in a microfluidic disk and quantification by fluorescence microscopy. Our platform is fast (20 min), sensitive (10 3 CFU/mL), requires minimal sample preparation, and can detect multiple pathogens simultaneously with sensitivity similar to that required by the EPA. We demonstrate detection of a panel of enteric bacteria (Escherichia coli, Salmonella typhimurium, Shigella, Listeria, and Campylobacter) at concentrations as low as 10 3 CFU/mL or 30 bacteria per reaction.

Authors:
 [1];  [2];  [3];  [2]
  1. Univ. of Texas Medical Branch, Galveston, TX (United States)
  2. Univ. of Texas Medical Branch, Galveston, TX (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE
OSTI Identifier:
1427213
Report Number(s):
SAND-2015-1581J
Journal ID: ISSN 1932-1058; 567398
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Accepted Manuscript
Journal Name:
Biomicrofluidics
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1932-1058
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Litvinov, Julia, Moen, Scott T., Koh, Chung-Yan, and Singh, Anup K. Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water. United States: N. p., 2016. Web. doi:10.1063/1.4939099.
Litvinov, Julia, Moen, Scott T., Koh, Chung-Yan, & Singh, Anup K. Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water. United States. doi:10.1063/1.4939099.
Litvinov, Julia, Moen, Scott T., Koh, Chung-Yan, and Singh, Anup K. Fri . "Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water". United States. doi:10.1063/1.4939099. https://www.osti.gov/servlets/purl/1427213.
@article{osti_1427213,
title = {Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water},
author = {Litvinov, Julia and Moen, Scott T. and Koh, Chung-Yan and Singh, Anup K.},
abstractNote = {Water-born pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. We present an innovative centrifugal microfluidic platform (SpinDx) for detection of bacterial pathogens using bead-based immunoassays. Our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a density-media in a microfluidic disk and quantification by fluorescence microscopy. Our platform is fast (20 min), sensitive (103 CFU/mL), requires minimal sample preparation, and can detect multiple pathogens simultaneously with sensitivity similar to that required by the EPA. We demonstrate detection of a panel of enteric bacteria (Escherichia coli, Salmonella typhimurium, Shigella, Listeria, and Campylobacter) at concentrations as low as 103 CFU/mL or 30 bacteria per reaction.},
doi = {10.1063/1.4939099},
journal = {Biomicrofluidics},
number = 1,
volume = 10,
place = {United States},
year = {2016},
month = {1}
}

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