Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution
Abstract
The beta-lactam antibiotics inhibit penicillin-binding proteins (PBPs) by forming a stable, covalent, acyl-enzyme complex. During the evolution from PBPs to Class A beta-lactamases, the beta-lactamases acquired Glu166 to activate a catalytic water and cleave the acyl-enzyme bond. Here we present three product complex crystal structures of CTX-M-14 Class A beta-lactamase with a ruthenocene-conjugated penicillin-a 0.85 angstrom resolution structure of E166A mutant complexed with the penilloate product, a 1.30 angstrom resolution complex structure of the same mutant with the penicilloate product, and a 1.18 angstrom resolution complex structure of S70G mutant with a penicilloate product epimer-shedding light on the catalytic mechanisms and product inhibition of PBPs and Class A beta-lactamases. The E166A-penilloate complex captured the hydrogen bonding network following the protonation of the leaving group and, for the first time, unambiguously show that the ring nitrogen donates a proton to Ser130, which in turn donates a proton to Lys73. These observations indicate that in the absence of Glu166, the equivalent lysine would be neutral in PBPs and therefore capable of serving as the general base to activate the catalytic serine. Together with previous results, this structure suggests a common proton relay network shared by Class A beta-lactamases and PBPs, from themore »
- Authors:
-
- Univ. of South Florida, Tampa, FL (United States). College of Medicine, Dept. of Molecular Medicine
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS). X-ray Science Division
- Univ. of Lodz (Poland). Dept. of Organic Chemistry, Faculty of Chemistry
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Institutes of Health (NIH); USDOE
- OSTI Identifier:
- 1426672
- Alternate Identifier(s):
- OSTI ID: 1409859
- Grant/Contract Number:
- AC02-06CH11357; ACB‐12002; AGM‐12006; DEC‐2013/11/B/ST5/00997
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Federation of European Biochemical Societies (FEBS) Journal
- Additional Journal Information:
- Journal Name: Federation of European Biochemical Societies (FEBS) Journal; Journal Volume: 285; Journal Issue: 1; Journal ID: ISSN 1742-464X
- Publisher:
- Federation of European Biochemical Societies
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; X-ray crystallography; CTX-M; Penicillin Binding Protein; Penilloate; Penicilloate
Citation Formats
Lewandowski, Eric M., Lethbridge, Kathryn G., Sanishvili, Ruslan, Skiba, Joanna, Kowalski, Konrad, and Chen, Yu. Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution. United States: N. p., 2017.
Web. doi:10.1111/febs.14315.
Lewandowski, Eric M., Lethbridge, Kathryn G., Sanishvili, Ruslan, Skiba, Joanna, Kowalski, Konrad, & Chen, Yu. Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution. United States. https://doi.org/10.1111/febs.14315
Lewandowski, Eric M., Lethbridge, Kathryn G., Sanishvili, Ruslan, Skiba, Joanna, Kowalski, Konrad, and Chen, Yu. Mon .
"Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution". United States. https://doi.org/10.1111/febs.14315. https://www.osti.gov/servlets/purl/1426672.
@article{osti_1426672,
title = {Mechanisms of proton relay and product release by Class A β-lactamase at ultrahigh resolution},
author = {Lewandowski, Eric M. and Lethbridge, Kathryn G. and Sanishvili, Ruslan and Skiba, Joanna and Kowalski, Konrad and Chen, Yu},
abstractNote = {The beta-lactam antibiotics inhibit penicillin-binding proteins (PBPs) by forming a stable, covalent, acyl-enzyme complex. During the evolution from PBPs to Class A beta-lactamases, the beta-lactamases acquired Glu166 to activate a catalytic water and cleave the acyl-enzyme bond. Here we present three product complex crystal structures of CTX-M-14 Class A beta-lactamase with a ruthenocene-conjugated penicillin-a 0.85 angstrom resolution structure of E166A mutant complexed with the penilloate product, a 1.30 angstrom resolution complex structure of the same mutant with the penicilloate product, and a 1.18 angstrom resolution complex structure of S70G mutant with a penicilloate product epimer-shedding light on the catalytic mechanisms and product inhibition of PBPs and Class A beta-lactamases. The E166A-penilloate complex captured the hydrogen bonding network following the protonation of the leaving group and, for the first time, unambiguously show that the ring nitrogen donates a proton to Ser130, which in turn donates a proton to Lys73. These observations indicate that in the absence of Glu166, the equivalent lysine would be neutral in PBPs and therefore capable of serving as the general base to activate the catalytic serine. Together with previous results, this structure suggests a common proton relay network shared by Class A beta-lactamases and PBPs, from the catalytic serine to the lysine, and ultimately to the ring nitrogen. Additionally, the E166A-penicilloate complex reveals previously unseen conformational changes of key catalytic residues during the release of the product, and is the first structure to capture the hydrolyzed product in the presence of an unmutated catalytic serine.},
doi = {10.1111/febs.14315},
journal = {Federation of European Biochemical Societies (FEBS) Journal},
number = 1,
volume = 285,
place = {United States},
year = {2017},
month = {11}
}
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