Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Abstract

We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $$\mu$$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [1]
+ Show Author Affiliations
  1. Purdue Univ., West Lafayette, IN (United States). Dept. of Physics and Astronomy
  2. Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry, Photosynthetic Antenna Research Center
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1470599
Alternate Identifier(s):
OSTI ID: 1425540
Grant/Contract Number:  
SC0001035; FG02-09ER16084; SC0001341
Resource Type:
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 89; Journal Issue: 3; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly)

Citation Formats

Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., and Savikhin, Sergei. Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer. United States: N. p., 2018. Web. doi:10.1063/1.5009468.
Copy to clipboard
Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., & Savikhin, Sergei. Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer. United States. https://doi.org/10.1063/1.5009468
Copy to clipboard
Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., and Savikhin, Sergei. Mon . "Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer". United States. https://doi.org/10.1063/1.5009468. https://www.osti.gov/servlets/purl/1470599.
Copy to clipboard
@article{osti_1470599,
title = {Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer},
author = {Stadnytskyi, Valentyn and Orf, Gregory S. and Blankenship, Robert E. and Savikhin, Sergei},
abstractNote = {We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $\mu$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.},
doi = {10.1063/1.5009468},
journal = {Review of Scientific Instruments},
number = 3,
volume = 89,
place = {United States},
year = {Mon Mar 12 00:00:00 EDT 2018},
month = {Mon Mar 12 00:00:00 EDT 2018}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1063/1.5009468
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Ultrafast Carbonyl Motion of the Photoactive Yellow Protein Chromophore Probed by Femtosecond Circular Dichroism
journal, September 2013

  • Mendonça, Lucille; Hache, François; Changenet-Barret, Pascale
  • Journal of the American Chemical Society, Vol. 135, Issue 39
  • DOI: 10.1021/ja404503q

Picosecond circular dichroism spectroscopy: a Jones matrix analysis
journal, January 1990

  • Xie, Xiaoliang; Simon, John D.
  • Journal of the Optical Society of America B, Vol. 7, Issue 8
  • DOI: 10.1364/josab.7.001673

Time-resolved circular dichroism of native oat phytochrome photointermediates
journal, June 1992

  • Bjorling, Sofie C.; Zhang, Chian Fan; Farrens, David L.
  • Journal of the American Chemical Society, Vol. 114, Issue 12
  • DOI: 10.1021/ja00038a020

The Fate of the Triplet Excitations in the Fenna–Matthews–Olson Complex
journal, April 2015

  • Kihara, Shigeharu; Hartzler, Daniel A.; Orf, Gregory S.
  • The Journal of Physical Chemistry B, Vol. 119, Issue 18
  • DOI: 10.1021/jp512222c

Recent advances in time resolved circular dichroism spectroscopy
journal, October 1987

  • Kliger, D. S.; Lewis, J. W.
  • Reviews of Chemical Intermediates, Vol. 8, Issue 4
  • DOI: 10.1007/bf03055509

Quantum coherence in photosynthesis
journal, January 2011

Effect of Spectral Density Shapes on the Excitonic Structure and Dynamics of the Fenna–Matthews–Olson Trimer from Chlorobaculum tepidum
journal, August 2016

  • Kell, Adam; Blankenship, Robert E.; Jankowiak, Ryszard
  • The Journal of Physical Chemistry A, Vol. 120, Issue 31
  • DOI: 10.1021/acs.jpca.6b03107

Birefringence effects in transient circular dichroism measurements with applications to the photolysis of carbon monoxyhemoglobin and carbon monoxymyoglobin
journal, August 1985

  • Einterz, C. M.; Lewis, J. W.; Milder, S. J.
  • The Journal of Physical Chemistry, Vol. 89, Issue 18
  • DOI: 10.1021/j100264a015

How to study proteins by circular dichroism
journal, August 2005

  • Kelly, Sharon M.; Jess, Thomas J.; Price, Nicholas C.
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1751, Issue 2
  • DOI: 10.1016/j.bbapap.2005.06.005

Nanosecond circular dichroism spectral measurements: extension to the far-ultraviolet region
journal, May 1993

  • Zhang, C. F.; Lewis, J. W.; Cerpa, R.
  • The Journal of Physical Chemistry, Vol. 97, Issue 21
  • DOI: 10.1021/j100123a009

Towards a coherent picture of excitonic coherence in the Fenna–Matthews–Olson complex
journal, July 2012

  • Fidler, Andrew F.; Caram, Justin R.; Hayes, Dugan
  • Journal of Physics B: Atomic, Molecular and Optical Physics, Vol. 45, Issue 15
  • DOI: 10.1088/0953-4075/45/15/154013

Broadband femtosecond circular dichroism spectrometer with white-light polarization control
journal, April 2010

  • Trifonov, Anton; Buchvarov, Ivan; Lohr, Andreas
  • Review of Scientific Instruments, Vol. 81, Issue 4
  • DOI: 10.1063/1.3340892

Broadband near UV to visible optical activity measurement using self-heterodyned method
journal, January 2011

  • Eom, Intae; Ahn, Sung-Hyun; Rhee, Hanju
  • Optics Express, Vol. 19, Issue 10
  • DOI: 10.1364/oe.19.010017

How Proteins Trigger Excitation Energy Transfer in the FMO Complex of Green Sulfur Bacteria
journal, October 2006

Conformational Changes in Photoexcited ( R )-(+)-1,1′-Bi-2-naphthol Studied by Time-Resolved Circular Dichroism
journal, September 2008

  • Niezborala, Claire; Hache, François
  • Journal of the American Chemical Society, Vol. 130, Issue 38
  • DOI: 10.1021/ja8039844

Evidence for wavelike energy transfer through quantum coherence in photosynthetic systems
journal, April 2007

  • Engel, Gregory S.; Calhoun, Tessa R.; Read, Elizabeth L.
  • Nature, Vol. 446, Issue 7137
  • DOI: 10.1038/nature05678

Allosteric Intermediates in Hemoglobin. 1. Nanosecond Time-Resolved Circular Dichroism Spectroscopy †
journal, January 1996

  • Björling, Sofie C.; Goldbeck, Robert A.; Paquette, Sarah J.
  • Biochemistry, Vol. 35, Issue 26
  • DOI: 10.1021/bi952247s

Linear dichroism and circular dichroism in photosynthesis research
journal, May 2009

Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies
journal, January 2012

  • Kliger, David S.; Chen, Eefei; Goldbeck, Robert A.
  • International Journal of Molecular Sciences, Vol. 13, Issue 1
  • DOI: 10.3390/ijms13010683

Ultrasensitive time-resolved linear dichroism spectral measurements using near-crossed linear polarizers
journal, July 1994

The Use of Circular Dichroism in the Investigation of Protein Structure and Function
journal, December 2000

  • Kelly, Sharon; Price, Nicholas
  • Current Protein & Peptide Science, Vol. 1, Issue 4, p. 349-384
  • DOI: 10.2174/1389203003381315

Nanosecond optical rotatory dispersion spectroscopy: application to photolyzed hemoglobin-CO kinetics
journal, January 1995

Perturbation of bacteriochlorophyll molecules in Fenna–Matthews–Olson protein complexes through mutagenesis of cysteine residues
journal, September 2016

  • Saer, Rafael; Orf, Gregory S.; Lu, Xun
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 9
  • DOI: 10.1016/j.bbabio.2016.04.007

Nanosecond time-resolved polarization spectroscopies: Tools for probing protein reaction mechanisms
journal, September 2010

Evidence for a cysteine-mediated mechanism of excitation energy regulation in a photosynthetic antenna complex
journal, June 2016

  • Orf, Gregory S.; Saer, Rafael G.; Niedzwiedzki, Dariusz M.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 31
  • DOI: 10.1073/pnas.1603330113

Exciton Analysis in 2D Electronic Spectroscopy
journal, June 2005

  • Cho, Minhaeng; Vaswani, Harsha M.; Brixner, Tobias
  • The Journal of Physical Chemistry B, Vol. 109, Issue 21
  • DOI: 10.1021/jp050788d

Analysis of optical artifacts in ellipsometric measurements of time-resolved circular dichroism
journal, June 1991

  • Bjorling, Sofie C.; Goldbeck, Robert A.; Milder, Steven J.
  • The Journal of Physical Chemistry, Vol. 95, Issue 12
  • DOI: 10.1021/j100165a018

The measurement of transient circular dichroism: A new kinetic technique
journal, November 1974

  • Ferrone, F. A.; Hopfield, J. J.; Schnatterly, S. E.
  • Review of Scientific Instruments, Vol. 45, Issue 11
  • DOI: 10.1063/1.1686510

Picosecond time‐resolved circular dichroism spectroscopy: experimental details and applications
journal, August 1989

  • Xie, Xiaoliang; Simon, John D.
  • Review of Scientific Instruments, Vol. 60, Issue 8
  • DOI: 10.1063/1.1140681

Communication: Broadband and ultrasensitive femtosecond time-resolved circular dichroism spectroscopy
journal, September 2015

  • Hiramatsu, Kotaro; Nagata, Takashi
  • The Journal of Chemical Physics, Vol. 143, Issue 12
  • DOI: 10.1063/1.4932229

Circular Dichroism Techniques: Biomolecular and Nanostructural Analyses- A Review
journal, August 2009

Application of time-resolved circular dichroism to the study of conformational changes in photochemical and photobiological processes
journal, May 2009

Effect of Diagonal Energy Disorder on Circular Dichroism Spectra of Fenna−Matthews−Olson Trimers
journal, October 1997

  • Buck, Daniel R.; Savikhin, Sergei; Struve, Walter S.
  • The Journal of Physical Chemistry B, Vol. 101, Issue 42
  • DOI: 10.1021/jp971758g

Time-resolved circular dichroism and absorption studies of the photolysis reaction of (carbonmonoxy)myoglobin
journal, May 1988

A picosecond circular dichroism study of photosynthetic reaction centers from Rhodobacter sphaeroides
journal, March 1991

Comparative Study of the Folding/Unfolding Dynamics of Poly(glutamic acid) in Light and Heavy Water
journal, May 2014

  • Mendonça, Lucille; Steinbacher, Andreas; Bouganne, Raphaël
  • The Journal of Physical Chemistry B, Vol. 118, Issue 20
  • DOI: 10.1021/jp501282z

Measurement of circular dichroism at millisecond time resolution: a stopped-flow circular dichroism system
journal, June 1974

New technique for measuring circular dichroism changes on a nanosecond time scale. Application to (carbonmonoxy)myoglobin and (carbonmonoxy)hemoglobin
journal, January 1985

  • Lewis, J. W.; Tilton, R. F.; Einterz, C. M.
  • The Journal of Physical Chemistry, Vol. 89, Issue 2
  • DOI: 10.1021/j100248a023

Nanosecond time‐resolved circular dichroism measurements using an upconverted Ti:sapphire laser
journal, September 1996

  • Wen, You‐Xian; Chen, Eefei; Lewis, James W.
  • Review of Scientific Instruments, Vol. 67, Issue 9
  • DOI: 10.1063/1.1147422

Chlorophyll arrangement in a bacteriochlorophyll protein from Chlorobium limicola
journal, December 1975

  • Fenna, R. E.; Matthews, B. W.
  • Nature, Vol. 258, Issue 5536
  • DOI: 10.1038/258573a0

Stopped-flow circular dichroism: A new fast-kinetic system
journal, February 1974

Measuring the dynamics of circular dichroism in a pump-probe experiment with a Babinet-Soleil compensator: erratum
journal, January 2007

  • Niezborala, Claire; Hache, François
  • Journal of the Optical Society of America B, Vol. 24, Issue 4
  • DOI: 10.1364/josab.24.001012

Revisiting the optical properties of the FMO protein
journal, March 2010

  • Milder, Maaike T. W.; Brüggemann, Ben; van Grondelle, Rienk
  • Photosynthesis Research, Vol. 104, Issue 2-3
  • DOI: 10.1007/s11120-010-9540-1

From Atomistic Modeling to Excitation Transfer and Two-Dimensional Spectra of the FMO Light-Harvesting Complex
journal, July 2011

  • Olbrich, Carsten; Jansen, Thomas L. C.; Liebers, Jörg
  • The Journal of Physical Chemistry B, Vol. 115, Issue 26
  • DOI: 10.1021/jp202619a

Intensity Dependence of the Excited State Lifetimes and Triplet Conversion Yield in the Fenna–Matthews–Olson Antenna Protein
journal, February 2014

  • Orf, Gregory S.; Niedzwiedzki, Dariusz M.; Blankenship, Robert E.
  • The Journal of Physical Chemistry B, Vol. 118, Issue 8
  • DOI: 10.1021/jp411020a

Exciton Simulations of Optical Spectra of the FMO Complex from the Green Sulfur Bacterium Chlorobium tepidum at 6 K
journal, November 1998

  • Vulto, Simone I. E.; de Baat, Michiel A.; Louwe, Robert J. W.
  • The Journal of Physical Chemistry B, Vol. 102, Issue 47
  • DOI: 10.1021/jp982095l

Fast Time-Resolved Magnetic Optical Rotatory Dispersion Measurements. 1. Mueller Analysis of Optical and Photoselection-Induced Artifacts
journal, November 1998

  • Esquerra, Raymond M.; Goldbeck, Robert A.; Kim-Shapiro, Daniel B.
  • The Journal of Physical Chemistry A, Vol. 102, Issue 45
  • DOI: 10.1021/jp981693x
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Ultrafast broadband circular dichroism in the deep ultraviolet
    journal, January 2019

    Ultrafast broadband circular dichroism in the deep ultraviolet
    text, January 2019

    • Oppermann, Malte; Bauer, Benjamin; Rossi, Thomas
    • Optical Society of America
    • DOI: 10.5167/uzh-172268
      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: